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pRSF_6His-ZmSUMO1a-GG_ZmSCE1b-Myc
Plasmid#91932PurposeBacterial expression of maize SUMO1a and SCE1bDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1b-Myc
UseTags6His and MycExpressionBacterialMutationTruncation of ZmSUMO1a after the diGlycine motifPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSF_6His-ZmSUMO1a-GG_ZmSCE1d-Myc
Plasmid#91934PurposeBacterial expression of maize SUMO1a and SCE1dDepositorInserts6His-ZmSUMO1a-GG
ZmSCE1d-Myc
UseTags6His and MycExpressionBacterialMutationTruncation of ZmSUMO1a after the diGlycine motifPromoterAvailable sinceJune 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pISceI Dusp6:memGFP
Plasmid#32668DepositorInsertDusp6 promoter (dusp6 Zebrafish)
UseZebrafish transgenesisTagsEGFPExpressionMutationPromoterAvailable sinceNov. 28, 2011AvailabilityAcademic Institutions and Nonprofits only -
Clathrin-LCa-ECFP
Plasmid#21742DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsECFPExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
yHS1
Plasmid#159150PurposeCytosolic labile heme reporterDepositorInsertyHS1
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAS1B
Plasmid#61968PurposeDuet vector for co-expression of GFP and mCherry from PBAD and PLtetO promoters, respectively; parent vector for split mCherry assembly vectorsDepositorInsertsmCherry
GFP
UseTagsExpressionBacterialMutationSerine 65 to Glycine (GGT), Serine 72 to Alanine …PromoterPBAD and PLtetOAvailable sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His6-Mps1-WT
Plasmid#114063Purposeprotein productionDepositorInsertMps1-WT (TTK Human)
UseTagsHis6ExpressionInsectMutationnonePromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG_EGFP-GFE3
Plasmid#79869PurposeExpresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.DepositorInsertGFP-GFE3
UseSynthetic BiologyTagsGFP and HAExpressionMammalianMutationPromoterBeta-actinAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C)
Plasmid#79045PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C)
UseTagsExpressionBacterialMutationA14C and E45C mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pISH-Barx1
Plasmid#84306PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Barx1. Not for gene expression.DepositorInsertBarx1 (Barx1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 aa 1-552
Plasmid#74614PurposeExpresses a truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-552 (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pHACK-GAL4>QF2(newHA1)
Plasmid#104873PurposeHACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of insertsDepositorInsertT2A-QF2
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
p35S-PBE
Plasmid#119772PurposeConversion of C to T in potatoDepositorInsertNLS-APOBEC1-nCas9-UGI-NLS
UseTagsNLSExpressionPlantMutationnCas9 has a mutation D10A in wild type SpCas9PromoterCaMv 2x35SAvailable sinceSept. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
CAGhP14
Plasmid#84362Purposeexpress FLAG-HA tagged human PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseTagsFLAG and HAExpressionMammalianMutationsilent mutations at coding silent 1068CtoT, 1629T…PromoterCAGAvailable sinceFeb. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-MAHS
Plasmid#90034PurposeTo express GFP-tagged MAHS in mammalian cells transientlyDepositorInsertMAHS
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV)
Plasmid#140160PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
(+)-Limonene Synthase
Plasmid#112230Purpose(+)-Limonene Synthase from Citrus sinensis in pET-28a(+). N-terminal was truncated to remove amino acids 1-52 and then His-tagged for purification.DepositorInsert(+)-Limonene Synthase
UseTagsN-terminal His-tagExpressionBacterialMutationdeleted amino acids 1-52PromoterAvailable sinceJune 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
