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pET-28b-Cas9-His

Plasmid

#47327

Purpose

For in vitro expression and purification of Cas9 protein

Depositor

Alex Schier

Article

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pET29b-IPP1-His

Plasmid

#124137

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Inorganic pyrophosphatase (IPP1 Budding Yeast)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pLV_hEF1a_rtTA3

Plasmid

#61472

Purpose

3rd generation lentiviral vector; constitutive expression of rtTA3 (TetON system)

Depositor

Ron Weiss

Article

Busskamp et al Mol Syst Biol. 2014 Nov 17;10(11):760. d

Insert

rtTA3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hEF1a

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO EYFP (AAV5)

Viral Prep

#27056-AAV5

Purpose

Ready-to-use AAV5 particles produced from pAAV-Ef1a-DIO EYFP (#27056). In addition to the viral particles, you will also receive purified pAAV-Ef1a-DIO EYFP plasmid DNA. EF1a-driven, cre-dependent EYFP expression. These AAV preparations are suitable purity for injection into animals.

Depositor

Karl Deisseroth

Promoter

Tags

EYFP (Cre-dependent)

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only
pYSEAP

Plasmid

#37326

Depositor

John Schiller

Article

Pastrana et al Virology. 2004 Apr 10;321(2):205-16.

Insert

SEAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

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pGL3 Control Vector

Plasmid

#212937

Purpose

Control vector with constitutive firefly luciferase expression

Depositor

Debrya Groskreutz

Insert

luc+

Use

Luciferase

Tags

Expression

Mutation

WT

Promoter

SV40

Available since

Jan. 16, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV INS2x mRFP-3MREs (AAV-beta)

Plasmid

#206928

Purpose

Beta cell-specific gene expression from AAV in human pancreatic islets

Depositor

Markus Grompe

Article

Chai et al Hum Gene Ther. 2022 Aug;33(15-16):789-80

Insert

mRFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

INSx2

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-KP1

Plasmid

#206504

Purpose

AAV packaging vector containing AAV2 rep and KP1 cap and partial AAV2 p5 promoter

Depositor

Mark Kay

Article

Pekrun et al JCI Insight. 2019 Nov 14;4(22). pii: 131

Insert

KP1 capsid

Use

AAV

Tags

Expression

Mutation

Promoter

p5

Available since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-PEmax for IVT

Plasmid

#178113

Purpose

Template for in vitro transcription of PEmax

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax

Use

Template for in vitro transcription

Tags

SV40 bpNLS and c-Myc NLS

Expression

Mutation

Detailed in manuscript

Promoter

T7 (inactivated)

Available since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pyEvolvR (enCas9-PolI5M)

Plasmid

#158073

Purpose

Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

Depositor

John Dueber

Article

Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

Insert

enCas9-PolI5M

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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aeBlue chromoprotein

Plasmid

#117846

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses aeBlue chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, aeBlue

Use

Synthetic Biology; Escherichia coli

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Egr1

Plasmid

#11729

Depositor

Eileen Adamson

Article

Yu et al Mol Cell. 2004 Jul 2. 15(1):83-94.

Insert

Egr-1 (Egr1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)Flag-His-ATM wt

Plasmid

#31985

Purpose

Mammalian expression of human ATM with Flag and His tags

Depositor

Michael Kastan

Article

Canman et al Science. 1998 Sep 11;281(5383):1677-9.

Insert

ATM wild-type (ATM Human)

Use

Tags

Flag and HIS

Expression

Mammalian

Mutation

3' UTR of 400 nucl.

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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6xHis-SNAP-PI(4,5)P2 Probe

Plasmid

#211513

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4,5-Bisphosphate (PI(4,5)P2) from bacteria

Depositor

David Murray, Hannes Maib

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

PLCdelta1-PH

Use

Tags

6xhis-SNAP

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pPLV_C1

Plasmid

#186415

Purpose

Endogenous murine RNase inhibitor production for cell-free lysate

Depositor

Jerome Bonnet

Article

Voyvodic et al Sci Rep. 2022 Aug 12;12(1):13785. doi: 1

Insert

Murine RNase Inhibitor

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pHR-scFv-GCN4-sfGFP-GB1-dWPRE

Plasmid

#60907

Purpose

The plasmid encodes an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP for imaging

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

scFv-GCN4

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-mCherry-(F30-2xPepper)10

Plasmid

#129401

Purpose

mCherry mRNA reporter tagged with (F30-2xPepper)10

Depositor

Samie Jaffrey

Article

Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

Insert

mCherry-(F30-2xPepper)10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 puro

Plasmid

#104994

Purpose

This 3rd generation lentiviral construct delivers hSpCas9 and puromycin resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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miniCMV-tdTomato-tDeg

Plasmid

#217521

Purpose

Expresses tdTomato-tDeg fluorogenic protein in mammalian cells with miniCMV promoter

Depositor

Xing Li

Article

Zhang et al Nat Commun. 2024 Jan 31;15(1):934. doi:

Insert

tdTomato-tDeg fluorogenic protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

miniCMV

Available since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCasPA

Plasmid

#113347

Purpose

Bacterial expression of Cas9 nuclease and λ-Red system in Pseudomonas aeruginosa

Depositor

Quanjiang Ji

Article

Chen et al iScience. 2018 Aug 31;6:222-231. doi: 10

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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