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Plasmid#199047PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpWKS1 ORI plus NGS barcode 17
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_2
Plasmid#199034PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpUB110 ORI plus NGS barcode 2
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_45
Plasmid#199052PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpNG2 ORI plus NGS barcode 22
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_4
Plasmid#199036PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpIP501 ORI plus NGS barcode 4
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL0_115 [pBC1 ORI]
Plasmid#199015PurposeLevel 0 partDepositorInsertpBC1 ORI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGLDO_1 [α-sfYFP-β]
Plasmid#199032PurposeLevel 1 plasmid, methylated YFP dropoutDepositorInsertα-sfYFP-β dropout part
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_44
Plasmid#199051PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpBBR1-UP ORI plus NGS barcode 21
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_9
Plasmid#199041PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpSC101ts ORI plus NGS barcode 10
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_1
Plasmid#199033PurposeLevel 1 plasmid, barcoded ORIDepositorInsertpAL5000 ORI plus NGS barcode 1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL1_47
Plasmid#199054PurposeLevel 1 plasmid, barcoded ORIDepositorInsertp15A ORI plus NGS barcode 24
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-4xArg[CGA]-nLuc
Plasmid#199759PurposeElongation reporter construct to quantify elongation duration of 4_CGA stallingDepositorInsertsynYFP[TTG/AGA]-4xArg[CGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[CGA]-nLuc
Plasmid#199760PurposeElongation reporter construct to quantify elongation duration of 6_CGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[CGA]-nLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG306-pTet07-synYFP[TTG/AGA]-6xArg[AGA]-nLuc
Plasmid#199761PurposeElongation reporter construct to quantify elongation duration of 6_AGA stallingDepositorInsertsynYFP[TTG/AGA]-6xArg[AGA]-cLuc
UseTagsExpressionYeastMutationPromoterTetO7Available sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-INS-sg
Plasmid#194719PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hINSDepositorArticleInsertAsCpf1 (INS Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-MAFB-sg
Plasmid#194718PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that target hMAFBDepositorArticleInsertAsCpf1 (MAFB Acidaminococcus sp.)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-CD86-mEGFP
Plasmid#190748PurposeExpression of CD86 double tagged with mCherry2 (N-term) and mEGFP (C-term) in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmCherry2 and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCherry2-CD86-mEYFP(Q69K)-mEGFP
Plasmid#190751PurposeExpression of triple tagged CD86 with mCherry2, mEYFP(Q69K), and mEGFP in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmCherry2, mEGFP, and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCD86-mEYFP(Q69K)-mEGFP
Plasmid#190750PurposeExpression of CD86 double tagged with mEYFP(Q69K) and mEGFP in mammalian cells.DepositorInsertCD86 (CD86 Human)
UseTagsmEGFP and mEYFP(Q69K)ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-TRE3G-NODALvar-matMYC
Plasmid#115641PurposeFor targeted integration and inducible expression of a human NODAL splice variant (with an N-terminal MYC tag on the mature peptide) using doxycyclineDepositorInsertNODAL splice variant (NODAL Human)
UseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
NODAL-MYC-DYK-C312A
Plasmid#115253PurposeFor mammalian expression of the human NODAL open reading frame (with a mutated Cysteine residue) with a C-terminal MYC-DYK tagDepositorInsertNODAL (NODAL Human)
UseTagsMYC-DYKExpressionMammalianMutationC312APromoterCMVAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only