162,890 results

1. pMD19-PBBa_J23117-sgRNA-Spr

   Plasmid

   #190793

   Purpose

   Template vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Insert

   sgRNA (dummy)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23117

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAGGS-EGFP-NLS-3XFLAG-V5

   Plasmid

   #196090

   Purpose

   Expresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tag

   Depositor

   Mauro Calabrese

   Article

   Cherney et al RNA. 2023 Jul 19:rna.079569.122. doi: 10

   Insert

   GFP
   

   Use

   Tags

   C-terminal 3xFLAG and C-terminal V5

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAGGs

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. mCherry-UVRAG

   Plasmid

   #86743

   Purpose

   N-terminal mCherry-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Kim et al Mol Cell. 2015 Jan 22;57(2):207-18. doi:

   Insert

   human UVRAG (UVRAG Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr

   Plasmid

   #190794

   Purpose

   Template vector to amplify pieces for creating multiplex sgRNA-arrays.

   Depositor

   Paul Hudson

   Article

   Cengic et al ACS Synth Biol. 2022 Aug 15. doi: 10.102

   Insert

   sgRNA-scaffold only
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23117

   Available since

   Nov. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-PKM2-R399E

   Plasmid

   #122691

   Purpose

   Express His-tagged PKM2 (R399E) protein in bacteria.

   Depositor

   Wen-Ching Wang

   Article

   Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

   Insert

   Pyruvate kinase M2 isoform (PKM Human)
   

   Use

   Tags

   6His-tag

   Expression

   Bacterial

   Mutation

   R399E

   Promoter

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCoofy51

   Plasmid

   #122005

   Purpose

   Baculovirus vector for parallel SLIC cloning containing N-terminal TwinStrep with PreScission Cleavage site

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   TwinStrep

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLCHKO_intergenic-intergenic_2

   Plasmid

   #155078

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA targeting two intergenic sites in the human genome using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCoofy60

   Plasmid

   #122012

   Purpose

   Baculovirus vector for parallel SLIC cloning containing N-terminal His6-eGFP with PreScission Cleavage site

   Depositor

   Sabine Suppmann, Peggy Stolt-Bergner

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His6-eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFH2.22_mCherry-MS2x8

   Plasmid

   #205537

   Purpose

   Express mCherry-MS2x8 (export tag consisting of 8 tandem repeats of MS2 stem-loop aptamer)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLCHKO_HPRT1_exon2_1_Lb

    Plasmid

    #155051

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA for deletion of HPRT1 exon 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28a-PKM2-H391Y

    Plasmid

    #122692

    Purpose

    Express His-tagged PKM2 (H391Y) protein in bacteria.

    Depositor

    Wen-Ching Wang

    Article

    Chen et al Commun Biol. 2019 Mar 15;2:105. doi: 10.

    Insert

    Pyruvate kinase M2 isoform (PKM Human)
    

    Use

    Tags

    6His-tag

    Expression

    Bacterial

    Mutation

    H391Y

    Promoter

    Available since

    March 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCnCre

    Plasmid

    #232907

    Purpose

    Electroporation vector for expression of Cre recombinase in Cuprividus necator

    Depositor

    Anita Emmerstorfer-Augustin

    Article

    Arhar et al ACS Synth Biol. 2025 Feb 24. doi: 10.102

    Insert

    Cre recombinase (cre Synthetic)
    

    Use

    Cre/Lox and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ptac-cymO

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pI3_EGFP

    Plasmid

    #239335

    Purpose

    Expresses the I3-01 nanocage subunit C-terminally tagged with EGFP in mammalian cells. Assembles into nanocages tagged with 60 EGFPs.

    Depositor

    Torsten Wittmann

    Insert

    I3-01
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-dCas13-NES-eNAT10

    Plasmid

    #238299

    Purpose

    Expresses cytoplasmic version of the programmable RNA acetylation system.

    Depositor

    Won Do Heo

    Article

    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

    Insert

    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    

    Use

    CRISPR

    Tags

    HIV NES (C terminal of dPspCas13b)

    Expression

    Mammalian

    Mutation

    H133A for catalytically inactive mutant, deletion…

    Promoter

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-NLS-dCas13-NLS-eNAT10

    Plasmid

    #238300

    Purpose

    Expresses nuclear version of the programmable RNA acetylation system.

    Depositor

    Won Do Heo

    Article

    Yu et al Nat Chem Biol. 2025 Jun 2. doi: 10.1038/

    Insert

    dPspCas13b fused to eNAT10 (NAT10 Prevotella sp. P5-125, Human)
    

    Use

    CRISPR

    Tags

    SV40 BPNLS and SV40 BPNLS (C terminal of dPspCas1…

    Expression

    Mammalian

    Mutation

    H133A for catalytically inactive mutant, deletion…

    Promoter

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmStayGold_mStayGold_I3

    Plasmid

    #239339

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with two copies of mStayGold in mammalian cells. Assembles into nanocages tagged with 120 FPs.

    Depositor

    Torsten Wittmann

    Insert

    I3-01
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available since

    June 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmScarlet3_I3

    Plasmid

    #239342

    Purpose

    Expresses the I3-01 nanocage subunit N-terminally tagged with mScarlet3 in mammalian cells. Assembles into nanocages tagged with 60 FPs.

    Depositor

    Torsten Wittmann

    Insert

    I3-01
    

    Use

    Tags

    mScarlet3

    Expression

    Mammalian

    Mutation

    K129A

    Promoter

    CMV

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. fabp10a:ER-CreER; cryaa:mCerulean

    Plasmid

    #230077

    Purpose

    Expression of ERCreER in zebrafish hepatocytes.

    Depositor

    Sumeet Pal Singh

    Article

    Hovhannisyan et al bioRxiv 2025.05.23.655316

    Insert

    ERT2-Cre-ERT2
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    fabp10a

    Available since

    May 30, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pAAV hSyn INTRON mCherry-Jph3 WPRE

    Plasmid

    #236237

    Purpose

    AAV expression of human Junctophilin3 N-terminally tagged with mCherry

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    Junctophilin 3 (JPH3 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available since

    May 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. SIV_Gag_Pol

    Plasmid

    #236243

    Purpose

    3rd generation SIV-based lentiviral packaging plasmid; Contains SIV Gag and Pol

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Benedetti et al Cell. 2025 Jan 23;188(2):484-500.e22. do

    Insert

    SIV Gag and Pol
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge