213,925 results

1. pETCK3 TGP-E

   Plasmid

   #236255

   Purpose

   Expresses TGP-E, a thermostable fluorescent protein

   Depositor

   Natasha DeVore

   Article

   Anderson et al Biochem Mol Biol Educ. 2024 Sep-Oct;52(5

   Insert

   Thermal Green Protein Q66E (TGP-E)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   lac operon

   Available since

   May 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-FBXL17

   Plasmid

   #236188

   Purpose

   Expression of N-terminal tagged FLAG-FBXL17 (H. sapiens, codon-optimized) in human cell lines.

   Depositor

   Michele Pagano

   Article

   Cao et al Cell. 2024 Oct 30:S0092-8674(24)01188-7.

   Insert

   FLAG-FBXL17 (FBXL17 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUGW U6 gLacZ dCas9-KRAB-T2a-GFP

   Plasmid

   #234883

   Purpose

   non-targeting CRISPRi control

   Depositor

   Johan Jakobsson

   Article

   Adami et al bioRxiv 2025.01.17.633315

   Insert

   L1HS gRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. GFP-hCGN

   Plasmid

   #236507

   Purpose

   For expression of Cingulin (human) in mammalian cells

   Depositor

   Sandra Citi

   Article

   Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

   Insert

   CGN (CGN Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. hCGNL1-Myc-HA

   Plasmid

   #236515

   Purpose

   For expression of hParacingulinL1 in mammalian cells

   Depositor

   Sandra Citi

   Article

   Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

   Insert

   CGNL1 (CGNL1 Human)
   

   Use

   Tags

   Myc-HA

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   April 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXs-mAscl1

   Plasmid

   #233152

   Purpose

   Retroviral expression of Ascl1

   Depositor

   Kate Galloway

   Article

   Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

   Insert

   Ascl1 (Ascl1 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Flag-ZDHHC13

   Plasmid

   #231661

   Purpose

   Expresses human ZDHHC13 protein

   Depositor

   Chunhong Ma

   Article

   Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

   Insert

   ZDHHC13 (ZDHHC13 Human)
   

   Use

   Tags

   3×Flag Tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAIP4_2WWE

   Plasmid

   #234210

   Purpose

   Heterologous protein expression of PI3K-C2-gamma in Escherichia coli

   Depositor

   Christopher Bahl

   Insert

   2WWE (PIK3C2G Human)
   

   Use

   Tags

   10x HisTag-Smt3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 6, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pSems StayGold-2xFYVE

   Plasmid

   #222948

   Purpose

   Expression of HRS 2xFYVE tagged with StayGold in mammalian cells

   Depositor

   Jacob Piehler

   Article

   Winkelmann et al Nat Commun. 2024 Jul 10;15(1):5813. doi:

   Insert

   tandem HRS FYVE domain (Hgs Mouse)
   

   Use

   Tags

   StayGold

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMB1: All in one SpCas9 expression and sgRNA cloning backbone

    Plasmid

    #228363

    Purpose

    mU6-driven expression of spCas9 sgRNAs with EF1a-SpCas9-2A-Puro-2A-mCherry

    Depositor

    Michael Boettcher

    Article

    El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; Spcas9 nuclease and spcas9…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-cT3G-cERP2-EZH2

    Plasmid

    #222528

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of EZH2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. sgLATS1/2-2

    Plasmid

    #229432

    Purpose

    knockout of LATS1 and LATS2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgLATS1/2-2 (LATS1, LATS2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV hU6-sgKRAS hUbC-dCas9-KRAB-T2a-GFP1

    Plasmid

    #184556

    Purpose

    Knockdown of murine KRAS gene by targeting the promoter region via CRISPRi system

    Depositor

    Sandeep Prabhu

    Article

    Qian et al Cell Rep. 2023 Jul 25;42(7):112794. doi:

    Insert

    Kirsten rat sarcoma virus (Kras Mouse)
    

    Use

    CRISPR, Lentiviral, and Mouse Targeting

    Tags

    Flag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG

    Plasmid

    #217713

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA3 (ADGRA3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-743

    Promoter

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcdna 3.1 DSG-3 Tailless Control

    Plasmid

    #182718

    Purpose

    expresses a truncated force-insensitive DSG-3 TSmod

    Depositor

    Daniel Conway

    Article

    Jin et al bioRxiv [Preprint]. 2024 May 6:2024.05.0

    Insert

    desmoglein-3 (Dsg3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    TSmod inserted at G726, downstream C-terminal tai…

    Promoter

    Available since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA5 TEX264wt-cGFP

    Plasmid

    #220211

    Purpose

    Mammalian expression of wild type TEX264 fused at C-terminus to GFP

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Use

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TetO

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. HA-MBP-TEVpCS-ADGRA2 CTFdeltaTA-FLAG

    Plasmid

    #217712

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA2 (ADGRA2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-752

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-MBP-TEVpCS-ADGRA1 CTFdeltaTA-FLAG

    Plasmid

    #217711

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA1 (ADGRA1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-6

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. EV-A71 2A protease; 6xHis-MBP-TEV tag, self cleaving

    Plasmid

    #204811

    Purpose

    Bacterial expresison of Enterovirus A71 2A protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    A71 2A protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pHYX137

    Plasmid

    #202814

    Purpose

    Saccharomyces cerevisiae vector for the expression of polycistronic genes. Expression controled by the auto-inducible yeast promoter PCK1. LEU2 selection.

    Depositor

    Jean-Félix Dallery

    Article

    Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScPCK1

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge