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Plasmid#62215PurposeUAS transgenesis vectorDepositorTypeEmpty backboneUseZebrafish transgenesisTagsExpressionMutationPromoterUAS-E1bAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only
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pSCAC-69
Plasmid#31241PurposeMitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafishDepositorInsertmitochondrial localization enhanced green fluorescent protein
UseTagsExpressionMutationPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pT1QciMP
Plasmid#62216PurposeQUAS transgenesis vectorDepositorTypeEmpty backboneUseZebrafish transgenesisTagsExpressionMutationPromoterQUAS-E1bAvailable sinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
p5E-dusp6
Plasmid#82584Purpose5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cellsDepositorInsertFGF-responsive element of the dusp6 promoter
UseTagsExpressionMutationPromoterNoneAvailable sinceDec. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hcar1-4-mKate2
Plasmid#226712Purposeoverexpression red fluorescent hcar1-4 protein in zebrafish larvaeDepositorInserthcar1-4
UseUnspecified; Zebrafish expressionTagsmKate2ExpressionMutationPromoterSP6Available sinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME-Cas9-T2A-GFP
Plasmid#63155PurposeMiddle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.DepositorInsertCas9-T2A-GFP
UseCRISPR; Tol2 middle entry vector for gatewayTagsExpressionMutationPromoterAvailable sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
th-P2A-EGFP donor
Plasmid#65562PurposeIntron targeting-mediated EGFP knockin at the zebrafish th locus.DepositorInsertth-P2A-EGFP (th Zebrafish)
UseZebrafish injectionTagsExpressionMutationPromoternoneAvailable sinceJune 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
th-P2A-Gal4 donor
Plasmid#65563PurposeIntron targeting-mediated Gal4 knockin at the zebrafish th locus.DepositorInsertth-P2A-Gal4 (th Zebrafish)
UseZebrafish injectionTagsExpressionMutationPromoternoneAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
acta1-Cavin1a-Clover
Plasmid#163852PurposeZebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPRDepositorInsertCavin1a (cavin1a Zebrafish)
UseZebrafish expressionTagsCloverExpressionMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:ChR2(H134R)-tdTomato
Plasmid#124237PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomatoDepositorInsertChR2(H134R)-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
MiniCoopR mitfa:SPRED1
Plasmid#118851PurposeExpresses human SPRED1 and zebrafish mitfa specifically in zebrafish melanocytesDepositorInsertSPRED1 (SPRED1 Human, Zebrafish)
UseZebrafish expression, tol7TagsExpressionMutationPromoterAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pminiTol2-4kb wnt3 promoter-Fzd1mApple
Plasmid#203795PurposeTol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesisDepositorInsertFzd1 (fzd1 Zebrafish)
UseZebrafish expressionTagsmAppleExpressionMutationPromoter4kb Wnt3 promoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-zgc:152904
Plasmid#192740PurposeGateway entry vector encoding zebrafish zgc:152904DepositorInsertzgc:152904 (zgc:152904 Zebrafish)
UseGateway entry vectorTagsExpressionMutationN56D; 663T_664DinsA; 733V_772Edel (exon 24)PromoterNoneAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-usp25
Plasmid#192733PurposeGateway entry vector encoding zebrafish usp25DepositorInsertusp25 (usp25 Zebrafish)
UseGateway entry vectorTagsExpressionMutationS403R; W622R; 725A_726AinsAA (rs508161559); A730T…PromoterNoneAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-psmg1
Plasmid#192723PurposeGateway entry vector encoding zebrafish psmg1DepositorInsertpsmg1 (psmg1 Zebrafish)
UseGateway entry vectorTagsExpressionMutationR96Q (rs514814252); T152M (rs501951686); T189A (r…PromoterNoneAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAWO-SUMO-3C_14M_zTDP2
Plasmid#200512PurposeExpression of zebrafish TDP2 recombinant proteinDepositorInsertTDP2 (tdp2b Zebrafish)
UseTagsHIS-SUMOExpressionBacterialMutationL136N, A139S, C239T, K240R, K309Q, K314L, T315G, …PromoterAvailable sinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-nrip1b
Plasmid#192732PurposeGateway entry vector encoding zebrafish nrip1bDepositorInsertnrip1b (nrip1b Zebrafish)
UseGateway entry vectorTagsExpressionMutationL661P (rs511527097); S720P; A801T; K862E; A863P; …PromoterNoneAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-Vector-Dendra2
Plasmid#97101Purposeempty vector for overexpression of microRNA and Dendra2 in ZebrafishDepositorTypeEmpty backboneUseZebrafishTagsExpressionMutationPromoterAvailable sinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR - CaSR-EGFP
Plasmid#191503PurposeA zebrafish CaSR-EGFP construct used for Gateway cloning.DepositorInsertCaSR-EGFP-SV40
UseZebrafish expressionTagsExpressionMutationPromoternoneAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST mfap4:turquoise2
Plasmid#135218PurposeCyan tagged macrophagesDepositorInsertmfap4 promoter and mTurquoise2
UseZebrafish expressionTagsExpressionMutationPromoterAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only