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Plasmid#146961PurposeInsect Expression of DmAGO1-R937DDepositorInsertDmAGO1-R937D (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-60-DmAgo1_572-984-optim_K
Plasmid#146728PurposeBacterial Expression of DmAgo1_572-984-optimDepositorInsertDmAgo1_572-984-optim (AGO1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28c(plus)-DmAGO1-RC_F
Plasmid#146262PurposeBacterial Expression of DmAGO1-RCDepositorInsertDmAGO1-RC (AGO1 Fly)
UseTagsExpressionBacterialMutationone non silent mutation R787C compared to sequenc…PromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmAGO2-RC_B
Plasmid#145980PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
UseTagsExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO2-RC_B
Plasmid#145981PurposeInsect Expression of DmAGO2-RCDepositorInsertDmAGO2-RC (AGO2 Fly)
UseTagsExpressionInsectMutationA deletion of AA 51-56 and an insertion of 23 AA …PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-RB_B
Plasmid#145977PurposeInsect Expression of DmAGO1-RBDepositorInsertDmAGO1-RB (AGO1 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F2V2_A
Plasmid#145896PurposeInsect Expression of DmAGO1-F2V2DepositorInsertDmAGO1-F2V2 (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F594V_A
Plasmid#145897PurposeInsect Expression of DmAGO1-F594VDepositorInsertDmAGO1-F594V (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-siRNAres_A
Plasmid#145899PurposeInsect Expression of DmAGO1-siRNAresDepositorInsertDmAGO1-siRNAres (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations compared to…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-K690A_M
Plasmid#146957PurposeInsect Expression of DmAGO1-K690ADepositorInsertDmAGO1-K690A (AGO1 Fly)
UseTagsExpressionInsectMutationvariation with 2 non silent mutations V315A and H…PromoterAvailable sinceFeb. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmAGO1-F629V_A
Plasmid#145898PurposeInsect Expression of DmAGO1-F629VDepositorInsertDmAGO1-F629V (AGO1 Fly)
UseTagsExpressionInsectMutationV315A, F629V, H913R - variation with 2 non silent…PromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSG5HA-MAGE-112-429
Plasmid#89131Purposemammalian expression of N-terminal HA-tagged human MAGE-A11 amino acid residues 112-429DepositorInsertMAGE-A11 (MAGEA11 Human)
UseTagsHAExpressionMammalianMutationcontains aa 112-429PromoterSV40Available sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MAGE-112-429
Plasmid#89128Purposemammalian expression of human MAGE-A11 amino acid residues 112-429 with N-terminal FLAG tagDepositorInsertMAGE-A11 (MAGEA11 Human)
UseTagsFLAGExpressionMammalianMutationcontains aa 112-429PromoterCMVAvailable sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI_E01_mini-mAgrin-Myc-His-WPRE__LG1-3
Plasmid#198141PurposeExpress mini-Agrin in mammalian cellsDepositorInsertmini-Agrin (Agrn Mouse)
UseTagsTEV-Myc-HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI_E01_mini-mAgrin-Myc-His-WPRE__LG3
Plasmid#198140PurposeExpress mini-Agrin in mammalian cellsDepositorInsertmini-Agrin (Agrn Mouse)
UseTagsTEV-Myc-HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-DIO-Magneto2.0-sNRPpA
Plasmid#74307PurposeCre-dependent expression of Magneto2.0 in mammalian cellsDepositorUseAAV and Cre/LoxTagsFlagExpressionMammalianMutationTRPV4: delta 760-871PromoterCMV (after Cre recombination)Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CG2-Neurog1-Magneto2.0-p2A-mCherry-pA
Plasmid#74302PurposeExpression of Magneto2.0-p2A-mCherry in Rohon-Beard sensory neurons of the zebrafishDepositorUseZebrafishTagsFLAG, cmcl2::EGFP, and p2A-mCherryExpressionMutationTRPV4: delta 760-871PromoterNeurog1Available sinceMay 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI_mini-mAgrin-mSA-Myc-His
Plasmid#198135PurposeExpress mini-Agrin in mammalian cellsDepositorInsertmini-Agrin (Agrn Mouse)
UseTagsmSA, Myc-HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceJune 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCI_E01_mini-mAgrin-Myc-His-WPRE__LG1
Plasmid#198138PurposeExpress mini-Agrin in mammalian cellsDepositorInsertmini-Agrin (Agrn Mouse)
UseTagsTEV-Myc-HisExpressionMammalianMutationPromoterCMV, beta-globin/IgG chimeric intronAvailable sinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS
Plasmid#121821PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9 (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only