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Showing: 141 - 160 of 239 results

  5. C98m

    Plasmid
    #121002
    Purpose
    MoClo golden gate assembly CD part for Gp2 (RNA polymerase inhibitor from T7 phage; lethal to host when expressed). Please see Supplemental Documents for annotated Genbank file.
    Depositor
    Richard Murray
    Insert
    Gp2 toxin
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121002-plasmid-map-sequence-id-236135

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  7. pET15bLCFPT7

    Plasmid
    #53545
    Purpose
    Activation module. The plasmid consists of a mutant T7 RNA polymerase (T7 RNAP) activating its own expression via a T7 promoter carrying alac operator. Carbenicillin resistant
    Depositor
    Lingchong You
    Article
    Payne et al Mol Syst Biol. 2013 Oct 8;9:697. doi: 10
    Insert
    T7 RNAP
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    53545-plasmid-map-sequence-id-83442

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  10. pRAV23

    Plasmid
    #67715
    Purpose
    Parental plasmid for transcription of RNAs with a GlmS/MS2 3'-tag for native purification
    Depositor
    Jeffrey Kieft, Robert Batey
    Article
    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Aug. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pRAV24

    Plasmid
    #67716
    Purpose
    Parental plasmid for transcription of RNAs with a GlmS/M-domain 3'-tag for native purification
    Depositor
    Jeffrey Kieft, Robert Batey
    Article
    Batey et al RNA. 2007 Aug;13(8):1384-9. Epub 2007 Ju
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Sept. 8, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pAJ1

    Plasmid
    #138379
    Purpose
    pEcRNAP6 derivative encoding Escherichia coli core RNA polymerase subunits: RpoA, RpoB, RpoC(His)10, RpoZ
    Depositor
    Seth Darst
    Article
    Cartagena et al Proc Natl Acad Sci U S A. 2019 Sep 17;11
    Inserts
    RpoA (rpoA E. coli)
    RpoB (rpoB E. coli)
    RpoC (rpoC E. coli)
    rpoZ (rpoZ E. coli)
    Use
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Clamp toe deletion: Δ(Tyr144-Lys179) ::(G144-S145…
    Promoter
    T7
    Available since
    April 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138379-plasmid-map-sequence-id-275999

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  14. pRAV12

    Plasmid
    #67713
    Purpose
    Parental plasmid for transcription of RNAs with a HdV/M-domain 3'-tag for native purification
    Depositor
    Jeffrey Kieft, Robert Batey
    Article
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Transcription cassette for 3'-tagged RNAs
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67713-plasmid-map-sequence-id-126588

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  16. pTmaM4

    Plasmid
    #67714
    Purpose
    Expression vector hexahistidine tagged variant of the Thermotoga maritima M-domain protein
    Depositor
    Jeffrey Kieft, Robert Batey
    Article
    Kieft et al RNA. 2004 Jun;10(6):988-95.
    Insert
    Thermotoga marítima Ffh-M domain
    Use
    Tags
    hexahistidine tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 RNA polymerase
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    67714-plasmid-map-sequence-id-126589

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Showing: 141 - 160 of 239 results