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MXS_PGK

Plasmid

#62414

Purpose

MXS_chaining vector with PGK

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

PGK Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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phpBCL2-FL(pA)

Plasmid

#42593

Depositor

Richard Lloyd

Article

Sherrill et al J Biol Chem. 2004 Jul 9;279(28):29066-74

Insert

hairpin - BCL2 5'UTR - firefly luciferase

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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Trip-GFP-hOCLN-ΔN

Plasmid

#32792

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

Deletion of aa 1-63

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pENTR4-FKBP12DD N-term B (w385-4)

Plasmid

#17415

Purpose

Gateway entry vector for Degron, N-term, frame B

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

FKBP12DD (L106P)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_Q355N_mCherry_BamUK

Plasmid

#119762

Purpose

Encodes mCherry-tagged BcLOV4 permanently-"lit" mutant with Q355N mutation for expression in bacterial cell

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-Q355N_mCherry

Use

Tags

mCherry

Expression

Bacterial

Mutation

Mutated glutamine at position 355 to asparagine

Promoter

T7

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b A146T

Plasmid

#83150

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), A146T mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

A146T

Promoter

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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Trip-GFP-hOCLN-ΔEL1

Plasmid

#32796

Depositor

Tianyi Wang

Article

Liu et al Virology. 2010 Nov 10;407(1):160-70. Epu

Insert

OCLN (OCLN Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Deletion of aa 94-128

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-shNup133-1

Plasmid

#87334

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

H1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF4D09

Plasmid

#70941

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED25 (MED25 Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
Hs.KRAS4a Q61L

Plasmid

#83171

Purpose

Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61L mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Q61L

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR/pTER shEGFP #1 (628-2)

Plasmid

#17470

Purpose

Gateway entry vector for EGFPshRNA#1 under H1/TO promoter

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #1

Use

RNAi; Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB-S577A

Plasmid

#92235

Purpose

Express S577A mutant of prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata-S577A mutant

Use

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

Serine 577 to Alanine

Promoter

T7 promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGEXndoS(E235Q)

Plasmid

#44656

Depositor

Mattias Collin

Article

Allhorn et al BMC Microbiol. 2008 . 8():3.

Insert

ndoS (ndoS Streptococcus pyogenes)

Use

Tags

Gst

Expression

Bacterial

Mutation

Changed Glutamic acid 235 to Glutamine

Promoter

tac

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4a Q61R

Plasmid

#83172

Purpose

Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), Q61R mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Q61R

Promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBM13h-sd4U

Plasmid

#179690

Purpose

Bacterial expression of base editor

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[sd4U] UGI-TALE3-DddA-G1397-N wildtype

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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MXS_Tet

Plasmid

#62422

Purpose

MXS_chaining vector with Tet

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline inducible promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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MXS_tTA2

Plasmid

#62429

Purpose

MXS_chaining vector with rTA2

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Tetracycline transactivator

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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His6_BcLOV4_C292A_mCherry_BamUK

Plasmid

#119761

Purpose

Encodes mCherry-tagged BcLOV4 "dark" mutant with C292A mutation for expression in bacterial cells

Depositor

Brian Chow

Article

Glantz et al Methods Enzymol. 2019;622:249-270. doi:

Insert

BcLOV4-C292A_mCherry

Use

Tags

mCherry

Expression

Bacterial

Mutation

Mutated cysteine at position 292 to alanine

Promoter

T7

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3F03

Plasmid

#70962

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Utx (Utx Fly)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
MXS_minCMV

Plasmid

#62418

Purpose

MXS_chaining vector with minCMV

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

Minimal CMV Promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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