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Showing: 1561 - 1580 of 8123 results

  1. pCRISPR3_BC4

    Plasmid
    #191859
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191859-plasmid-map-sequence-id-379162

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  2. pCRISPR3_BC3

    Plasmid
    #191858
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191858-plasmid-map-sequence-id-379160

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  3. pCRISPR3_BC1

    Plasmid
    #191856
    Purpose
    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.
    Depositor
    Kimberly Reynolds
    Article
    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    191856-plasmid-map-sequence-id-379154

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  4. pLY86

    Plasmid
    #130949
    Purpose
    Engineered sgRNA-LEA2-WTmis2 generator circuit including promoter Plux2, 2 BoxB aptamers and 2 mis-matches in sgRNA scaffold
    Depositor
    Baojun Wang
    Article
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Insert
    sgRNA-LEA2-WTmis2
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Plux2
    Available since
    Oct. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    130949-plasmid-map-sequence-id-258930

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  5. pEPOR2KN0078

    Plasmid
    #117645
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9(pEPOR1CB0113), sgRNA{AT1G73460}(pEPOR1CB0099) and sgRNA{AT1G73460}(pEPOR1CB0110) to Protein kinase superfamily protein.
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    117645-plasmid-map-sequence-id-240245

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  6. pEPOR2KN0076

    Plasmid
    #117643
    Purpose
    Level2 MoClo construct, containing level1 plant expression cassettes for eSaCas9 (pEPOR1CB0113), sgRNA {AT1G62600}(pEPOR1CB0098) and sgRNA PPR-Adenylate Cyclase {AT1G62590}(pEPOR1CB0109)
    Depositor
    Nicola Patron
    Article
    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do
    Insert
    [35S:eSaCas9(pEPOR1CB0113) ] +[AtU6-26:sgRNA]
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    117643-plasmid-map-sequence-id-228395

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  7. pBA950

    Plasmid
    #122239
    Purpose
    CROP-seq vector optimized for sgRNA expression and CRISPRi activity. This vector expresses a eGFP-NT2 sgRNA with modified U6 promotor and sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    BFP selectable marker, plus a modified mU6 promoter and sgRNA constant region for optimized CRISPRi activity
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122239-plasmid-map-sequence-id-292012

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  8. pBA900

    Plasmid
    #122237
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs2 incorporated at the 3' end of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs2 at the 3' end of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    122237-plasmid-map-sequence-id-292010

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  9. pJR89

    Plasmid
    #140096
    Purpose
    sgRNA constant region and hU6 insert for programmed dual sgRNA cloning. The sgRNA constant region contains a capture sequence (cs1) in the stem loop for direct capture Perturb-seq.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA constant region CR3 with cs1 in stem loop and hU6 promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    140096-plasmid-map-sequence-id-292016

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  10. pJR85

    Plasmid
    #140095
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region. BsmBI sites were removed to allow for programmed dual sgRNA library cloning.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140095-plasmid-map-sequence-id-292013

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  11. pXPR_219

    Plasmid
    #164559
    Purpose
    Lentiviral vector that encodes two sgRNA expression cassettes. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Article
    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR and Lentiviral; Dual sgrna
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    164559-plasmid-map-sequence-id-330205

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  12. pBA904

    Plasmid
    #122238
    Purpose
    Lentiviral CRISPR guide vector expressing a eGFP-NT2 sgRNA with cs1 incorporated in the loop of the sgRNA constant region.
    Depositor
    Jonathan Weissman
    Article
    Replogle et al Nat Biotechnol. 2020 Aug;38(8):954-961.
    Insert
    sgRNA with cs1 in stem loop 2 of the constant region
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    122238-plasmid-map-sequence-id-292011

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  13. eSpCas9(1.1)_NoFlag_hROSA26_Left

    Plasmid
    #191442
    Purpose
    Expresses the hROSA26 left sgRNA in combination with FLAGless eSpCas9(1.1) to target the hRosa26 safe harbor locus
    Depositor
    Yannick Doyon
    Insert
    hROSA26 sgRNA
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191442-plasmid-map-sequence-id-378118

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  14. HCP2

    Plasmid
    #166104
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166104-plasmid-map-sequence-id-330375

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  15. HCP3

    Plasmid
    #166105
    Purpose
    This plasmid encodes a Cas9 protein as well as a sgRNA that targets Msn2, which will create a double strand break and allow C-terminus tagging via homologous recombination
    Depositor
    Matthias Heinemann
    Insert
    Msn2-sgRNA#27 (MSN2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166105-plasmid-map-sequence-id-330319

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  18. pLentiCRISPRv2-sgAAVS1-HepmTurquoise2

    Plasmid
    #192828
    Purpose
    Expresses sgRNA targeting AAVS1 (non-targeting control sgRNA for mouse) and mTurquoise2 from hepatocyte-specific promoter
    Depositor
    Kristin Knouse
    Article
    Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do
    Insert
    mTurquoise2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for sgRNA and hepatocyte-specific promoter (HS…
    Available since
    Nov. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    192828-plasmid-map-sequence-id-378004

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  19. pTC217

    Plasmid
    #70018
    Purpose
    Cas9/sgRNA targeting ANT1 locus, donor molecule with 5' homology arm-Pnos:NptII-35S:ANT13' homology arm as a Gemini Viral Replicon based on Bean Yellow Dwarf Virus; sgRNA= 1b
    Depositor
    Daniel Voytas
    Article
    Cermak et al Genome Biol. 2015 Nov 6;16(1):232. doi:
    Insert
    Nuclease (Cas9/sgRNA) + Donor + GVR
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    March 8, 2016
    Availability
    Academic Institutions and Nonprofits only
    70018-plasmid-map-sequence-id-124557

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Showing: 1561 - 1580 of 8123 results