pK7-g7g8-K8
(Plasmid
#218220)
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PurposeAllows users to clone two Spacer sequences into an arrray of AtU6-promoter driven sgRNAs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 218220 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepICH41308
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Backbone manufacturerAddgene #47998
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAtU6 promoters and sgRNA scaffolds
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gRNA/shRNA sequenceUnspecified
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SpeciesA. thaliana (mustard weed)
Cloning Information
- Cloning method Golden Gate
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pK7-g7g8-K8 was a gift from Daniel Voytas (Addgene plasmid # 218220 ; http://n2t.net/addgene:218220 ; RRID:Addgene_218220)