Skip to main content
Addgene
Showing: 1561 - 1580 of 4474 results

  1. AbVec2.0-mIghg3

    Plasmid
    #127160
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg3 (Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hCMV IE1 gene promoter
    Available since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127160-plasmid-map-sequence-id-256904

    Enlarge

  2. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid
    #86668
    Purpose
    for generation of BirA tagged strains using CRISPR tagging technology
    Depositor
    David Sibley
    Insert
    Linker-BirA-3xHA-HXGPRT 3'UTR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    86668-plasmid-map-sequence-id-190141

    Enlarge

  3. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    86552-plasmid-map-sequence-id-190138

    Enlarge

  4. pUA97

    Plasmid
    #69340
    Purpose
    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy
    Depositor
    Oliver Hobert
    Insert
    ceBFP SEC 3xFlag
    Use
    Tags
    3xFlag and ceBFP (mTagBFP)
    Expression
    Worm
    Mutation
    sqt-1(e1350)
    Promoter
    Available since
    Dec. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    69340-plasmid-map-sequence-id-133241

    Enlarge

  5. pINER2R

    Plasmid
    #185882
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (11 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B>RPL25(partial; Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185882-plasmid-map-sequence-id-370829

    Enlarge

  8. pAW8-aid-3HA-eDSR-Turg1:kanMX6

    Plasmid
    #169363
    Purpose
    C-terminal aid-3HA-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    3XHA
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169363-plasmid-map-sequence-id-332979

    Enlarge

  9. pAW8-aid-5Myc-eDSR-Turg1:kanMX6

    Plasmid
    #169364
    Purpose
    C-terminal aid-5Myc-eDSR degron cassette with kanMX6 marker for S. pombe
    Depositor
    Tony Carr
    Insert
    auxin-inducible degron (aid)
    Use
    Tags
    5XMyc
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169364-plasmid-map-sequence-id-332978

    Enlarge

  15. pINER3R

    Plasmid
    #185883
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PPDA1>RPL25(part;Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    185883-plasmid-map-sequence-id-430023

    Enlarge

  16. pIAeBlueHPV16LR

    Plasmid
    #185894
    Purpose
    In vivo gene amplification (HamAmp) of AeBlue + HPV16 L1 expression module at RPL25 locus
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-TRPL25(Arm3)-ARS305-PALD6>EforRed>TPGK1-PSe.GAL2>HPV16-L1_C-6*H>TRPL41B-PBTS1>RPL25(partial;Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Nov. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    185894-plasmid-map-sequence-id-374392

    Enlarge

  17. pIT7A6

    Plasmid
    #185896
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module at RPL25 locus (15 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm 1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm 3)-ARS305-PSk.GAL2>Y.FAST-EVBR1.2A>TRPL41B-PPDA1>RPL25(partial;Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185896-plasmid-map-sequence-id-370384

    Enlarge

  18. pINER4R

    Plasmid
    #185884
    Purpose
    In vivo gene amplification (HamAmp) of nerolidol synthetic module locus (25 copy).
    Depositor
    Claudia Vickers
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185884-plasmid-map-sequence-id-370832

    Enlarge

  19. pPOU5F1-donor

    Plasmid
    #176367
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    POU5F1 (POU5F1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176367-plasmid-map-sequence-id-347873

    Enlarge

  20. pTRERF1-donor

    Plasmid
    #176366
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Kevin White, Barbara Stranger
    Insert
    TRERF1 (TRERF1 Human)
    Use
    CRISPR
    Tags
    GFP
    Expression
    Mutation
    Promoter
    Available since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176366-plasmid-map-sequence-id-347418

    Enlarge

Showing: 1561 - 1580 of 4474 results