Addgene

We narrowed to 2,262 results for: PRK

Showing: 1501 - 1520 of 2262 results

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pLTR PKC epsilon

Plasmid

#8420

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Sep 25;268(27):20110-5.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2007

Availability

Academic Institutions and Nonprofits only
pDONR221-SARA

Plasmid

#67346

Purpose

hSara with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
TAL2324

Plasmid

#36750

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MAP2K4-TALEN_Left (Map2k4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2325

Plasmid

#36751

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MAP2K4-TALEN_Right (Map2k4 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
p663-UBC-miniTurbo-V5-LMTK2_IDG-K

Plasmid

#170580

Purpose

Gateway destination clone of LMTK2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-LMTK2 (LMTK2 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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myc-Miro2 (lacking TM)

Plasmid

#127614

Purpose

Human Miro2 with the transmembrane deleted

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Miro2 (RHOT2 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Transmembrane deletion

Promoter

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Halo-Tev-Akt3 C119S-2X Flag

Plasmid

#87608

Purpose

expresses halo tagged at the N-terminus and 2 tandem flag tags at the C-terminus of C119S mutant of human Akt3 protein

Depositor

Yimon Aye

Insert

AKT3 isoform 2 (AKT3 Human)

Use

Zebrafish (danio rerio) expression

Tags

2 tandem flag tags and His-Halo-Tev

Expression

Mammalian

Mutation

cysteine 119 residue mutated to serine

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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isoform PKC Epsilon (JCE592)

Plasmid

#89776

Purpose

yeast expression of mouse PKC Epsilon with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC Epsilon (Prkce Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

yeast PKC1 promoter (-600 to -1)

Available since

May 3, 2017

Availability

Academic Institutions and Nonprofits only

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isoform PKC Eta (JCE615)

Plasmid

#89774

Purpose

yeast expression of mouse PKC Eta with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Article

Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

Insert

PKC Eta (Prkch Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

yeast PKC1 promoter (-600 to -1)

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST14-ARF4

Plasmid

#67371

Purpose

Expression of native hArf4 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-SARA-no stop

Plasmid

#67347

Purpose

hSara without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Gateway entry vector

Tags

Expression

Bacterial

Mutation

Promoter

None

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pcdna3 EE Jip1b

Plasmid

#52127

Purpose

a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.

Depositor

Roger Davis

Article

Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.

Insert

JNK interacting protein-1b (JIP-1b) (Mapk8ip1 Mouse)

Use

Tags

EE

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2014

Availability

Academic Institutions and Nonprofits only
anti-FLAG M2 heavy chain

Plasmid

#175359

Purpose

Mammalian expression vector for over-expression of anti-FLAG M2 heavy chain (C-terminal His8)

Depositor

Joost Snijder

Article

Peng et al J Proteome Res. 2021 Jul 2;20(7):3559-35

Insert

anti-FLAG M2 heavy chain

Use

Tags

His8 tag and N-terminal secretion signal peptide …

Expression

Mammalian

Mutation

L51I (see paper)

Promoter

CMV

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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HA-Beclin 1

Plasmid

#86748

Purpose

N-terminal HA-tagged protein expression in mammalian cells

Depositor

Do-Hyung Kim

Article

Park et al Autophagy. 2016;12(3):547-64. doi: 10.10

Insert

human Beclin 1 (BECN1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-p38beta

Plasmid

#86833

Purpose

mammalian expression of GFP-p38beta

Depositor

Rony Seger

Article

Zehorai et al Mol Cell Biol. 2014 Jan;34(2):259-70. do

Insert

p38beta (MAPK11 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3_altPKA-Cα-mEGFP

Plasmid

#114132

Purpose

Alternative spliced variant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric EGFP.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

18747 (Prkaca Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Parkin

Plasmid

#213547

Purpose

Transient mammalian expresssion of untagged Parkin

Depositor

Lani Wu, Steven Altschuler

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-ERK5

Plasmid

#53175

Purpose

Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
GST-BHMT-I/G

Plasmid

#104442

Purpose

Autophagy cargo assay

Depositor

Carol Mercer

Article

Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

Insert

BHMT (BHMT Human)

Use

Tags

GFP-myc GFP is from Aequorea victoria and GST

Expression

Mammalian

Mutation

N43A in GST (in a potential KFERQ-like sequence).…

Promoter

CMV

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pBMN-mCherry-Parkin

Plasmid

#59419

Purpose

to make mCherry-Parkin stable cell line with retrovirus

Depositor

Richard Youle

Article

Yamano et al Elife (Cambridge). 2014 Feb 25;3:e01612.

Insert

mCherry-Parkin (PRKN Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2014

Availability

Academic Institutions and Nonprofits only

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