Addgene

We narrowed to 1,706 results for: Xpa

Showing: 1381 - 1400 of 1706 results

Results per page:

W118-1_hSTK16-Flag

Plasmid

#180403

Purpose

lentiviral transduction of human STK16 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

STK16 (STK16 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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W118-1_Flag-hSRPK3

Plasmid

#180404

Purpose

lentiviral transduction of human SRPK3 (with an N-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

SRPK3 (SRPK3 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
W118-1_hTGM1-Flag

Plasmid

#180406

Purpose

lentiviral transduction of human TGM1 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM1 (TGM1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
W118-1_hTGM2-Flag

Plasmid

#180407

Purpose

lentiviral transduction of human TGM2 transcript variant 2 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2 (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
W118-1_hTGM5-Flag

Plasmid

#180410

Purpose

lentiviral transduction of human TGM5 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM5 (TGM5 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
W118-1_hTGM6-Flag

Plasmid

#180411

Purpose

lentiviral transduction of human TGM6 (with a C-terminal Flag tag) gene

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM6 (TGM6 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
W118-1_hTGM2_C277S-Flag

Plasmid

#180412

Purpose

lentiviral transduction of human TGM2 transcript variant 2 with a point mutation (C277S) that inactivate the enzyme and also with a C-terminal Flag tag

Depositor

Huda Zoghbi

Article

Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

Insert

TGM2_C277S (TGM2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

C277S

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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pAS_4xEcoLeuT(CUA)_EF1_AnapRS

Plasmid

#174894

Purpose

AnapRS expression for amber suppression in mammalian cells; transient or piggy bac mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AnapRS

Use

Tags

Expression

Mammalian

Mutation

L38F, M40G, L41P, Y499V, Y500L, Y527A, H537E, L53…

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xKBstTyrT(CUA)_EF1_sfGFP 102TAG 150TAA

Plasmid

#174895

Purpose

amber and ochre dual suppression reporter sfGFP 102TAG 150 TAA expression, with BstTyr(CUA) amber suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Use

Tags

Expression

Mammalian

Mutation

102TAG 150TAA in sfGFP

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pCS2-zfKitlga

Plasmid

#168199

Purpose

Express zebrafish Kitlga, mRNA synthesis

Depositor

Petr Bartunek

Article

Oltova et al Blood Adv. 2020 Dec 8;4(23):5915-5924. d

Insert

Kitlga (kitlga Zebrafish)

Use

Vertebrate expression (zebrafish, chicken, xenopu…

Tags

Expression

Mammalian

Mutation

F198L - please see depositor comments

Promoter

Availability

Academic Institutions and Nonprofits only

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pTD74

Plasmid

#172898

Purpose

Muscle promoter unc-54P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on chromosome I

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTD75

Plasmid

#172899

Purpose

Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi4348 site on LGI

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTD81

Plasmid

#172902

Purpose

Hypodermal promoter col-10P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pTD82

Plasmid

#172903

Purpose

Muscle promoter unc-54P::TIR1::F2A::TagBFP2::AID* with homology arms for insertion in ttTi5605 site on chromosome II

Depositor

David Reiner

Article

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

TIR-1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AttB_ACE2_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190076

Purpose

express human ACE2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

none

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[HA]_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190080

Purpose

express cytoplasmically HA-tagged human ACE2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

none

Availability

Academic Institutions and Nonprofits only

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AAV_pMBP-dnVamp2-P2AT2A-EGFP-caax

Plasmid

#190153

Purpose

Expresses dominant-negative Vamp2 (mouse Vamp2 AA 1-94) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

Depositor

Brad Zuchero

Article

Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

Insert

Vamp2 (Vamp2 Mouse)

Use

AAV

Tags

P2AT2A-EGFP-caax

Expression

Mammalian

Mutation

Truncation that includes only AA #1-94

Promoter

MBP

Availability

Academic Institutions and Nonprofits only

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pSBbi G418R mbIL21

Plasmid

#194329

Purpose

Consitiutive mammalian expression of membrane bound human IL-21

Depositor

Alexander McLellan

Article

Dobson et al Immunol Cell Biol. 2023 Aug 16. doi: 10.

Insert

mbIL-21 (IL21 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1-a

Availability

Academic Institutions and Nonprofits only

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pCK669

Plasmid

#192638

Purpose

Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dxCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

SoxS has R93A and S101A mutations and dxCas9-NG h…

Promoter

BBa_J23107 and Sp.pCas9

Availability

Academic Institutions and Nonprofits only

Enlarge
pCK340

Plasmid

#192639

Purpose

Expresses dSpG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dSpG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

SoxS has R93A and S101A mutations and dSpG has se…

Promoter

BBa_J23107 and Sp.pCas9

Availability

Academic Institutions and Nonprofits only

Enlarge