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Plasmid#62364PurposepFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5DepositorInsertBirA-mVenus-Ubc6 tail anchor
UseTagsExpressionYeastMutationL232H mutation compared to standard mVenusPromoterSec63Available sinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMLS719
Plasmid#188894PurposeminiMos Arm sgRNA expression vectorDepositorInsertPU6(K09B11.12)::miniMos Arm sgRNA
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTL032
Plasmid#80928Purpose14x MS2 sense + 14x PP7 antisense with loxP-kanMX-loxPDepositorInsert14xMS2 sense + 14xPP7 antisense, loxP-kanMX-loxP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJMP2480
Plasmid#160080PurposeZ. mobilis CRISPRi, promoter C, empty guideDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-dCer3-C2-FRB
Plasmid#191327PurposeExpresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-C2-dCer3
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-dCer3-C2-dCer3
Plasmid#191328PurposeExpresses the fluorescently labeled membrane anchor C2 without the chemical dimerization domain FRB as a control for recruiting proteins to plasma membraneDepositorInsertdCer3-C2-dCer3
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-FRB-dCer3-CAAX
Plasmid#191326PurposeExpresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRBDepositorInsertFRB-dCer-CAAX
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-mScarlet
Plasmid#191324PurposeExpresses fluorescent labeled beta Tubulin to measure microtubule densityDepositorInsertTUBB2a-mScarlet (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBetaActin-TUBB2a-Dronpa
Plasmid#191321PurposeExpresses fuorescently labeled beta tubulin to allow photoactivation of microtubule patches with very little UV lightDepositorInsertTUBB2a-Dronpa (Tubb2a Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMLS713
Plasmid#188885PurposeMultisite Gateway [4-3] Destination vector; miniMos transposon with lox2272-flanked Cbr-unc-119 markerDepositorTypeEmpty backboneUseMultisite gateway [4-3] destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJMP2132
Plasmid#160075PurposeZ. mobilis CRISPRi, promoter B, empty guideDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2046
Plasmid#160073PurposeZ. mobilis CRISPRi, promoter A, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pNM220_AAVS1 integration helper
Plasmid#211864PurposeCas9 cutting at AAVS1 safe harbor locusDepositorInsertAAVS1
UseCRISPRTagsExpressionMutationN/APromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDWJ27
Plasmid#207604PurposeGFP-BIB expressionDepositorInsertGFP-BIB
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-SENP3
Plasmid#115521PurposeMammalian expression constructDepositorInsertSENP3 (Senp3 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
SUMO_TwinStrep_Cas7-11_crRNA
Plasmid#197612PurposeFor protein purification of Cas7-11DepositorInsertSUMO_TwinStrep_Cas7-11 with crRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDF_6xHIS_Csx29
Plasmid#197613PurposeFor protein purification of Csx29DepositorInsert6-HIS tagged Csx29
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_dCas7-11
Plasmid#197605PurposeExpresses inactive Cas7-11 in mammalian cellsDepositorInsertdCas7-11
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_Gap43_Csx30_Cre
Plasmid#197604PurposeCre reporter for mammalian cells with Gap43 membrane anchorDepositorInsertGap43_Csx30_Cre
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_Cas7-11_T2A_Csx29
Plasmid#197607PurposeExpresses Cas7-11 and Csx29 in mammalian cellsDepositorInsertCas7-11_T2A_Csx29
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only