Skip to main content
Addgene
Showing: 1301 - 1320 of 50900 results

  1. pAS2-his-24::Cerulean

    Plasmid
    #124324
    Purpose
    Expressing Nuclear Localized Cerulean
    Depositor
    Andrew Leifer
    Insert
    Cerulean (his-24 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    124324-plasmid-map-sequence-id-241700

    Enlarge

  2. pAS2-his-24::tagBFP2

    Plasmid
    #124322
    Purpose
    Expressing Nuclear Localized tagBFP2
    Depositor
    Andrew Leifer
    Insert
    tagBFP2 (his-24 Nematode)
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    124322-plasmid-map-sequence-id-241690

    Enlarge

  3. PG1424PGA

    Plasmid
    #74653
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PG1424PGA
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. psicheck_85_CA_repeat

    Plasmid
    #65881
    Purpose
    Luciferase assay vector, assessing regulation of 85 nucleotide CA repeats
    Depositor
    Thomas Tuschl
    Insert
    CA repeats 85 nucleotides in length
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. psicheck_SRM1_3UTR

    Plasmid
    #65883
    Purpose
    Luciferase assay vector, assessing regulation of SRM1 3' UTR
    Depositor
    Thomas Tuschl
    Insert
    SRM1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  6. EcNGRfEfC-R10S1B9

    Bacterial Strain
    #48895
    Purpose
    diversity was introduced via homologous recombination to 40 sites to improve fatty acid production. Sequencing of the strains at these sites is ongoing.
    Depositor
    George Church
    Bacterial Resistance
    Spectinomycin
    Available since
    Dec. 3, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pCMV-Spike

    Plasmid
    #173921
    Purpose
    Expresses the original SARS-Cov-2 Spike Protein under a CMV Promoter
    Depositor
    Andreas Stuermer
    Insert
    SARS-2 Spike Protein (S SARS-COV-2, Human, Synthetic)
    Use
    Tags
    no tags, native protein
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    standard CMV
    Available since
    April 11, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    173921-plasmid-map-sequence-id-358849

    Enlarge

  8. HA-Clover-HIF-1alpha Wildtype

    Plasmid
    #163365
    Purpose
    Express HA- and Clover-tagged HIF-1alpha wildtype plasmid
    Depositor
    Violaine Sée
    Insert
    Hypoxia Inducible Factor 1 alpha (HIF1A Human)
    Use
    Tags
    HA- and Clover-tagged
    Expression
    Mammalian
    Mutation
    Wildtype
    Promoter
    CMV Promoter
    Available since
    Jan. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    163365-plasmid-map-sequence-id-322246

    Enlarge

  9. pJAT32

    Plasmid
    #204297
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. Two U6 promoters.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204297-plasmid-map-sequence-id-405508

    Enlarge

  10. pU6-Universal

    Plasmid
    #52694
    Purpose
    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.
    Depositor
    Sebastian Lourido
    Inserts
    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    Use
    CRISPR; Toxoplasma gondii
    Tags
    3X FLAG and Nuclear localization signal
    Expression
    Mutation
    Promoter
    TgTUB1 and Toxoplasma U6
    Available since
    June 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    52694-plasmid-map-sequence-id-82051

    Enlarge

  11. pGL4.10/RSV_XS3B-miniXon_Luciferase

    Plasmid
    #217302
    Purpose
    Plasmid containing the SF3B3-XS3B/Firefly luciferase cassette, a modification of the SF3B3-miniXon system. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-XS3B by L
    Depositor
    Beverly L. Davidson
    Insert
    XS3B-miniXon
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    217302-plasmid-map-sequence-id-436485

    Enlarge

  12. pE_Cre_lambdaCos

    Plasmid
    #173785
    Purpose
    The pE_Cre_lambdaCos plasmid is used to remove the antibiotic resistance cassette from pIT5 integrants.
    Depositor
    Keith Shearwin
    Article
    Hao et al ACS Synth Biol. 2021 Jun 30. doi: 10.102
    Insert
    Cre
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mutation
    S102Y and S279Y- please see depositor comments
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    173785-plasmid-map-sequence-id-342369

    Enlarge

  14. pJAT77

    Plasmid
    #204295
    Purpose
    pJAT plasmid with multiple cloning site for introducing custom payload. No U6 promoter. Restriction sites present to introduce flanking U6 promoters.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204295-plasmid-map-sequence-id-406178

    Enlarge

  17. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid
    #159908
    Purpose
    Mutagenesis of Htr2a
    Depositor
    Larry Zweifel
    Insert
    Htr2a (Htr2a Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159908-plasmid-map-sequence-id-317314

    Enlarge

  18. ACE2 g1 + Cas9

    Plasmid
    #153011
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Has Service
    Lentiviral Prep
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153011-plasmid-map-sequence-id-298673

    Enlarge

  19. ACE2 g4 + Cas9

    Plasmid
    #153014
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + ACE2 gRNA (ACE2 Human)
    Use
    Lentiviral
    Tags
    TagBFP2
    Expression
    Mutation
    Promoter
    Available since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153014-plasmid-map-sequence-id-298677

    Enlarge

Showing: 1301 - 1320 of 50900 results