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dCas9 plasmid

Plasmid

#100091

Purpose

dCas9 expression plasmid without effector fusions; 3X Flag tag; 2NLS; pCDNA3 vector backbone, mammalian expression

Depositor

David Segal

Article

O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99

Type

Empty backbone

Use

CRISPR

Tags

3XFlag-NLS-dCas9-NLS

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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Sender plasmid

Plasmid

#127677

Purpose

Encodes the synthase LuxI for the quorum-sensing molecule C6-HSL.

Depositor

Friedrich Simmel

Article

Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

Insert

LuxI

Use

Synthetic Biology

Tags

LVA

Expression

Bacterial

Mutation

Promoter

pT7

Availability

Academic Institutions and Nonprofits only

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KO plasmid

Plasmid

#135970

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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rescue plasmid

Plasmid

#135971

Purpose

expresses copGFP and puromycin resistance genes

Depositor

Yongming Wang

Article

Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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CelTag Plasmid

Plasmid

#66562

Purpose

Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

Depositor

David Engelke

Article

Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

Insert

CelTag

Use

Tags

Expression

Bacterial

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Plasmid EUPp_09

Plasmid

#157745

Purpose

PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

Depositor

Kang Zhou

Article

Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

Inserts

acetaldehyde dehydrogenase
alcohol dehydrogenase (ADH2 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

PgyrA

Availability

Academic Institutions and Nonprofits only

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CMV plasmid

Plasmid

#169739

Purpose

Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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CMV+ plasmid

Plasmid

#169740

Purpose

Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pCMV-tetO2

Availability

Academic Institutions and Nonprofits only

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PGK plasmid

Plasmid

#169744

Purpose

Unregulated "open-loop" PGK promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

eGFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

PGK promoter

Availability

Academic Institutions and Nonprofits only

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UBC plasmid

Plasmid

#169746

Purpose

Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

Depositor

Francois St-Pierre

Article

Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

Insert

EGFP

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Availability

Academic Institutions and Nonprofits only

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Noodles Plasmid

Plasmid

#212613

Purpose

An all-in-one system for on-target efficiency analysis of CRISPR guide RNAs

Depositor

Ilya Vinnikov

Article

Lin et al MethodsX. 2023 Nov 15;12:102481. doi: 10

Type

Empty backbone

Use

CRISPR and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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ATP1A1_plasmid_donor_RD

Plasmid

#86551

Purpose

Homology-directed repair (HDR) donor plasmid with homology arms to ATP1A1 to introduce the Q118R and N129D (RD) mutations conferring cellular resistance to ouabain

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 (ATP1A1 Human)

Use

CRISPR and TALEN; Co-selection via hdr using ouab…

Tags

Expression

Mammalian

Mutation

Q118R, N129D

Promoter

Availability

Academic Institutions and Nonprofits only

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ptetmCherry_prey_plasmid-2

Plasmid

#34617

Depositor

Lingchong You

Article

Song et al Nat Chem Biol. 2009 Dec;5(12):929-35. Ep

Insert

mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

ptet

Availability

Academic Institutions and Nonprofits only

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Sender_plasmid1

Plasmid

#140692

Purpose

Encodes constitutive yellow (sfYFP) fluorescence for identification

Depositor

Matthew Bennett

Article

Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

Insert

sfYFP

Use

Tags

ssrA degradation tag (ASV)

Expression

Bacterial

Mutation

Promoter

pIq constitutive promoter

Availability

Academic Institutions and Nonprofits only

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Sender_plasmid2

Plasmid

#140693

Purpose

Encodes inducible expression of RhlI (C4-HSL quorum sensing synthase)

Depositor

Matthew Bennett

Article

Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

Insert

RhlI (rhlI )

Use

Tags

ssrA degradation tag (ASV)

Expression

Bacterial

Mutation

Promoter

engineered lac promoter (IPTG inducible)

Availability

Academic Institutions and Nonprofits only

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Receiver_plasmid1

Plasmid

#140694

Purpose

Encodes constitutive red (mCherry) fluorescence for identification

Depositor

Matthew Bennett

Article

Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

Insert

mCherry

Use

Tags

ssrA degradation tag (ASV)

Expression

Bacterial

Mutation

Promoter

pIq constitutive promoter

Availability

Academic Institutions and Nonprofits only

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Reciever_plasmid2

Plasmid

#140695

Purpose

Encodes quorum sensing (C4-HSL) inducible cyan (sfCFP) expression

Depositor

Matthew Bennett

Article

Alnahhas et al ACS Synth Biol. 2019 Sep 20;8(9):2051-20

Insert

sfCFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

engineered rhl promoter (responds to C4-HSL)

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 1

Plasmid

#128569

Purpose

for mixing with SPECS library to produce lentivirus

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Insert

ECFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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Accessory plasmid 2

Plasmid

#128571

Purpose

for cloning identified individual SPECS

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TagRFP

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pSB4k5_RNAnano (r-plasmid)

Plasmid

#127634

Purpose

Encodes an RNA nanostructure with MG, PP7, TAR and S1m RNA aptamers. Transcription via T7 RNAP.

Depositor

Friedrich Simmel

Article

Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

Insert

RNA nanostrcutre

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

T7 RNAP

Availability

Academic Institutions and Nonprofits only

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