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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 23057 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRW95-3
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Backbone manufacturerPMID: 8800671
- Backbone size w/o insert (bp) 8700
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePR
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Alt nameprogesterone receptor
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SpeciesH. sapiens (human)
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Insert Size (bp)3000
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Entrez GenePGR (a.k.a. NR3C3, PR)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (destroyed during cloning)
- 3′ cloning site SnaBI (destroyed during cloning)
- 5′ sequencing primer GAL1 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The cDNA used to clone this plasmid was obtained from K. Horwitz
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRR-PR-5Z was a gift from Charles Miller (Addgene plasmid # 23057 ; http://n2t.net/addgene:23057 ; RRID:Addgene_23057) -
For your References section:
Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Plasmid. 2009 Dec 3. ():. 10.1016/j.plasmid.2009.11.003 PubMed 19962400