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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 23061 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRW95-3
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Backbone manufacturerPMID: 8800671
- Backbone size w/o insert (bp) 8700
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameERalpha
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Alt nameestrogen receptor alpha
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SpeciesH. sapiens (human)
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Insert Size (bp)3000
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Entrez GeneESR1 (a.k.a. ER, ESR, ESRA, ESTRR, Era, NR3A1)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SnaBI (destroyed during cloning)
- 3′ cloning site SnaBI (destroyed during cloning)
- 5′ sequencing primer GAL1 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe cDNA used to create this plasmid was obtained from Keith R. Yamamoto.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a M517T mutation in the ESR1 sequence. The mutation should not have any effect on protein function and this construct worked the same when assessed in yeast signaling assays and estrogen signaling.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRR-ERalpha-5Z was a gift from Charles Miller (Addgene plasmid # 23061 ; http://n2t.net/addgene:23061 ; RRID:Addgene_23061) -
For your References section:
Single plasmids expressing human steroid hormone receptors and a reporter gene for use in yeast signaling assays. Miller CA, Tan X, Wilson M, Bhattacharyya S, Ludwig S. Plasmid. 2009 Dec 3. ():. 10.1016/j.plasmid.2009.11.003 PubMed 19962400
Map uploaded by the depositor.