Addgene

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pSK+ mBMPR-1B DN

Plasmid

#49530

Purpose

cloning vector for dominant negative mouse BMP receptor 1B

Depositor

Lee Niswander, Peter ten Dijke

Article

Zou et al Genes Dev. 1997 Sep 1;11(17):2191-203.

Insert

BMPR-1B DN (Bmpr1b Mouse)

Use

Tags

Expression

Bacterial

Mutation

dominant negative mutation (K231R); also L2V

Promoter

T3

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-GSDMD_NoCys-Myc

Plasmid

#201169

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

C39A, C57A, C77A, C122A, C265A, C192A

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p40)

Plasmid

#135945

Purpose

Expresses AUF1p40 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p42)

Plasmid

#135946

Purpose

Expresses AUF1p42 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Use

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221-MICU1-sh1-rORF

Plasmid

#31764

Depositor

Vamsi Mootha

Article

Perocchi et al Nature. 2010 Sep 16;467(7313):291-6. Epu

Insert

MICU1 (MICU1 Human)

Use

Pdonr gateway

Tags

None

Expression

Mutation

RNAi-resistant ORF (rORF). 8 synonymous changes i…

Promoter

None

Available since

May 4, 2012

Availability

Academic Institutions and Nonprofits only

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pAG247

Plasmid

#33200

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

LRIG1 (LRIG1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
pEMS2275

Plasmid

#111893

Purpose

High Copy number plasmid containing Ple345 (NEFL MiniPromoter) insert.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

Ple345 (NEFL Human)

Use

Unspecified

Tags

Expression

Mutation

Promoter

Ple345 (NEFL MiniPromoter)

Available since

Aug. 10, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pCAG-p21_S146A

Plasmid

#40205

Depositor

Philipp Mergenthaler

Article

Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

Insert

p21 (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

S146A

Promoter

CAG

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-p21_S146D

Plasmid

#40206

Depositor

Philipp Mergenthaler

Article

Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

Insert

p21 (CDKN1A Human)

Use

Tags

Expression

Mammalian

Mutation

S146D

Promoter

CAG

Available since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pEMS1980

Plasmid

#49111

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS1993

Plasmid

#49123

Purpose

AAV plasmid with OLIG1 (Ple304) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple304-iCre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

OLIG1

Available since

May 6, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-DYRK4-2A-GFP

Plasmid

#118288

Purpose

Expresses mouse DYRK4 tagged with 2A peptide at it's C-terminus and GFP in mammalian cells.

Depositor

Vance Lemmon

Article

Slepak et al Cytoskeleton (Hoboken). 2012 Jul;69(7):5

Insert

dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (Dyrk4 Mouse)

Use

AAV

Tags

2A peptide and EGFP

Expression

Mammalian

Mutation

Promoter

CMV with beta global intron

Available since

Nov. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL11-GFP

Plasmid

#67407

Purpose

Mammalian expression of hArl11 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

bp 442 T to C; aa C148R

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pGL-p21UTRm1

Plasmid

#20878

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 1 mutation (Cdkn1a Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Predicted miRNA binding site 1 (Position ~420) GC…

Promoter

Available since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only
pEMS2158

Plasmid

#70119

Purpose

Promoterless AAV plasmid with MCS to insert promoter for expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-MCS-EmGFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Promoterless

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-MED9

Plasmid

#15407

Depositor

Joan Conaway, Ronald Conaway

Article

Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

Insert

MED9 (Med9 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FL MALT1 Catalytic Mutant in pET29b-Strep

Plasmid

#48969

Purpose

Expresses Full length catalytic mutant MALT1 in e.coli

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 isoform A (MALT1 Human)

Use

Tags

His and Strep

Expression

Bacterial

Mutation

Catalytic Mutant C464A

Promoter

T7

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only
mPapaya-Calnexin-N-14

Plasmid

#56343

Purpose

Localization: Endoplasmic Reticulum, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

Calnexin (CANX Human)

Use

Tags

mPapaya

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pGL-p21UTRm2

Plasmid

#20879

Depositor

Robert Blelloch

Article

Wang et al Nat Genet. 2008 Dec . 40(12):1478-83.

Insert

p21 3'UTR site 2 mutation (Cdkn1a Mouse)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Predicted miRNA binding site 2 (Position ~1100) G…

Promoter

Available since

March 12, 2012

Availability

Academic Institutions and Nonprofits only
pMKO shRNA Bmf

Plasmid

#17236

Depositor

Joan Brugge

Article

Schmelzle et al Proc Natl Acad Sci U S A. 2007 Mar 6. 10

Insert

Bmf shRNA (BMF Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only