mPapaya-Calnexin-N-14
(Plasmid
#56343)
-
PurposeLocalization: Endoplasmic Reticulum, Excitation: 530, Emission: 541
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 56343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonemPapaya-N1
- Backbone size w/o insert (bp) 4750
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameCalnexin
-
Alt nameNM_001746.3
-
SpeciesH. sapiens (human)
-
MutationI474N in Calnexin
-
Entrez GeneCANX (a.k.a. CNX, IP90, P90)
- Promoter CMV
-
Tag
/ Fusion Protein
- mPapaya (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
. Excitation = 530; Emission = 541
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
mPapaya-Calnexin-N-14 was a gift from Michael Davidson (Addgene plasmid # 56343 ; http://n2t.net/addgene:56343 ; RRID:Addgene_56343) -
For your References section:
An engineered monomeric Zoanthus sp. yellow fluorescent protein. Hoi H, Howe ES, Ding Y, Zhang W, Baird MA, Sell BR, Allen JR, Davidson MW, Campbell RE. Chem Biol. 2013 Oct 24;20(10):1296-304. doi: 10.1016/j.chembiol.2013.08.008. Epub 2013 Oct 3. 10.1016/j.chembiol.2013.08.008 PubMed 24094838