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Addgene

pSK+ mBMPR-1B DN
(Plasmid #49530)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 49530 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript SK+
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 2959
  • Total vector size (bp) 4500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BMPR-1B DN
  • Alt name
    alk-6
  • Alt name
    BMPR-1B
  • Species
    M. musculus (mouse)
  • Mutation
    dominant negative mutation (K231R); also L2V
  • Entrez Gene
    Bmpr1b (a.k.a. AI385617, ALK-6, AV355320, Acv, Acvrlk6, Al, Alk6, BMPR, BMPR-1B, BMPR-IB, CFK-43, CFK-43a, SKR6)
  • Promoter T3

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The BMPR-1B insert in this plasmid contains a L2V mutation when compared to GenBank reference sequence NP_031586.1. This mutation is not a concern for the function of the plasmid, as described in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSK+ mBMPR-1B DN was a gift from Lee Niswander & Peter ten Dijke (Addgene plasmid # 49530 ; http://n2t.net/addgene:49530 ; RRID:Addgene_49530)
  • For your References section:

    Distinct roles of type I bone morphogenetic protein receptors in the formation and differentiation of cartilage. Zou H, Wieser R, Massague J, Niswander L. Genes Dev. 1997 Sep 1;11(17):2191-203. 10.1101/gad.11.17.2191 PubMed 9303535