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Addgene
Showing: 1281 - 1300 of 147311 results
  1. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Insert
    SMAD exon 9 gRNA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
  2. 4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7 probasin_mTQ2_FlpO

    Plasmid
    #68356
    Purpose
    -The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and SMAD4 ex2. are expressed by the U6 promoter
    Inserts
    4xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    Synthetic Probasin ARRx2 and U6
    Availability
    Academic Institutions and Nonprofits only
  3. 5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9 probasin_mTQ2_FlpO

    Plasmid
    #68357
    Purpose
    The prostate specific promoter controls expression of FlpO linked to a blue flourescent protein. gRNA towards PTEN ex5, p53 ex7 and 8, and SMAD4 ex2 and ex 9 are expressed by the U6 promoter.
    Inserts
    5xgRNA: PTEN exon 5, p53 exon 8, SMAD4 exon 2, p53 exon 7, SMAD4 exon 9
    FlpO recombinase
    Use
    CRISPR
    Tags
    mTurquoise2 (BFP)
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and synthetic Probasin ARRx2
    Availability
    Academic Institutions and Nonprofits only
  4. pMPO 52

    Plasmid
    #68359
    Purpose
    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII, ColE1 replication origin
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  5. pMPO 53

    Plasmid
    #68360
    Purpose
    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII-sspH2 signal peptide, ColE1 replication origin
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  6. pMPO 57

    Plasmid
    #68361
    Purpose
    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-nasF attenuator-MCSII, ColE1 replication origin
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. pMPO 58

    Plasmid
    #68362
    Purpose
    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm- nasF attenuator-T7 SD sequence-MCSII, ColE1 replication origin
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pMPO 59

    Plasmid
    #68364
    Purpose
    Expression vectors for the salycilate cascade expression system (Pnah/NahR-Psal/XylS2).ApR, expression vector with rrnBT1T2-Pm-T7 SD sequence-MCSII-sspH2 signal peptide, ColE1 replication origin
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  9. TriEx-4 DHX36

    Plasmid
    #68368
    Purpose
    Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.
    Depositor
    Insert
    DHX36 (DHX36 Human)
    Use
    Tags
    Histidine
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    Promoter
    CMV ie enhancer/promoter
    Availability
    Academic Institutions and Nonprofits only
  10. PGR-Blue

    Plasmid
    #68374
    Purpose
    Terminator quantification vector utilizing Golden Gate Assembly and amilCP. Positive colonies are green and false positives are blue. Terminators are placed between GFP and RFP - induced by arabinose.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology; Golden gate assembly
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Availability
    Academic Institutions and Nonprofits only
  11. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid
    #68375
    Purpose
    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    3xFLAG_Twin_Strep
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK1
    Availability
    Academic Institutions and Nonprofits only
  12. EGFP-Alpha-5-Integrin-10

    Plasmid
    #56423
    Purpose
    Localization: Focal Adhesions, Excitation: 488, Emission: 507
    Depositor
    Insert
    Alpha-5 Integrin (ITGA5 Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 1281 - 1300 of 147311 results