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pMSCVpuro-Venus

Plasmid

#96940

Purpose

Retroviral plasmid containing Venus for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Venus Fluorescent Protein

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR221-CTSB

Plasmid

#158443

Purpose

Gateway Cloning compatible entry vector for the human CTSB gene.

Depositor

Sho Iketani, Alejandro Chavez

Insert

CTSB (CTSB Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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PNCA-luciferase

Plasmid

#67793

Purpose

Use together with pCMV-intron and VSV-G to package MMLV reporter virus

Depositor

Stephen Goff

Article

Wang et al J Virol. 2015 Mar;89(5):2813-9. doi: 10.

Insert

MMLV-LTR-firefly luciferase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-D-Topo-hTRIM9

Plasmid

#51032

Purpose

entry vector for cloning human TRIM9

Depositor

Timothy Cox

Article

Short et al J Biol Chem. 2006 Mar 31;281(13):8970-80

Insert

TRIM9 (TRIM9 Human)

Use

Entry vector

Tags

Expression

Mutation

Promoter

NA

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

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6his-WT gusA-pET28a+

Plasmid

#61156

Purpose

N -terminal His tagged wild-type GUS expression vector

Depositor

Ichiro Matsumura, Andrew Ellington

Article

Matsumura et al Nat Biotechnol. 1999 Jul;17(7):696-701.

Insert

gusA

Use

Tags

6x His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b-miniE-DBCO

Plasmid

#181985

Purpose

Used to label proteins with the MiniR genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

MiniE-DBCO

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-miniR-DBCO

Plasmid

#181984

Purpose

Used to label proteins with the MiniE genetically encoded tag

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

MiniR-DBCO

Use

Tags

DBCO-Tag, His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pICXNHG3

Plasmid

#49021

Purpose

FX cloning Pichia pastoris expression vector with AOX1 promoter and N-terminal 10x His tag, GFP and 3C protease cleavage site

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Yeast

Mutation

Promoter

AOX1

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR/pSM2(U6) (w51-1)

Plasmid

#17387

Purpose

Gateway entry vector for miRNA expression under U6 promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

RNAi; Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pJK225

Plasmid

#71124

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA TfR1-miniE

Plasmid

#181987

Purpose

MiniE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with MiniR labeled with a reporter

Depositor

Kimberly Beatty

Article

Doh et al Biochemistry. 2020 Aug 25;59(33):3051-30

Insert

TfR1-MiniE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pJExpress414_GmPOPB

Plasmid

#92234

Purpose

Express wild type prolyl oligopeptidase from Galerina marginata

Depositor

James Naismith

Article

Czekster et al Biochemistry. 2017 Apr 18;56(15):2086-20

Insert

Prolyl oligopeptidase B from Galerina marginata

Use

Tags

6x-Histag, TEV cleavage site

Expression

Bacterial

Mutation

none

Promoter

T7 promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA

Plasmid

#47069

Purpose

FX cloning E.coli expression vector with araBAD promoter, N-terminal 10x His tag, 3C protease cleavage site and C-terminal Avi-tag

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), AVI-tag, and…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBXCA3GH

Plasmid

#47071

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal Avi-tag, 3C protease cleavage site, GFP and 10x His tags

Depositor

Eric Geertsma, Raimund Dutzler

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), Avi-tag, GFP…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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aCE120

Plasmid

#204491

Purpose

Figure 1. reporter circuit

Depositor

Ron Weiss

Article

Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

Inserts

EBFP2
mKate2
EYFP

Use

Synthetic Biology

Tags

none

Expression

Bacterial and Mammalian

Mutation

Promoter

hEF1a

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4-GST 6P-1 (w487-1)

Plasmid

#17741

Purpose

Gateway entry vector for N-term GST fusion, frame 6P-1

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Entry vector

Tags

GST

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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AP1

Plasmid

#179678

Purpose

Accessory plasmids

Depositor

David Liu

Article

Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

Insert

[SD8] gIII

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-mApple

Plasmid

#96934

Purpose

Retroviral plasmid containing mApple for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Monomeric Apple

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR4-V5-LUC (w468-1)

Plasmid

#19135

Purpose

Gateway entry vector, V5-Luciferase

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Firefly Luciferase

Use

Entry vector

Tags

V5

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBAD-hsGFP

Plasmid

#64911

Purpose

Encodes a Fluorescent Reporter for Hydrogen Sulfide in E. Coli

Depositor

Huiwang Ai

Article

Chen et al Biochemistry. 2014 Sep 23;53(37):5966-74

Insert

hsGFP

Use

Tags

His Tag

Expression

Bacterial

Mutation

Chromophore tyrosine of the protein mutated to TA…

Promoter

araBAD

Available since

May 20, 2015

Availability

Academic Institutions and Nonprofits only

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