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Plasmid#60954Purpose2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherryDepositorInsertKRAB-dCas9-P2A-mCherry fusion
UseCRISPR and LentiviralTags2x NLS, HA, KRAB domain, and mCherryExpressionMammalianMutationPromoterSFFVAvailable sinceJan. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry
Plasmid#64324PurposeExpression vector for sgRNAs cloned into the BbsI sites and for expression of Cas9 linked to mCherry via a T2A peptideDepositorInsertsCas9
sgRNA cassette
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianMutationPromoterCBh and U6Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA
Plasmid#37082PurposeCre-dependent expression of hChR2(H134R) for optogenetic excitationDepositorInserthChr2(H134R)-mCherry
UseAAV and Cre/Lox; Cre-offTagsmCherryExpressionMutationPromoterEF1aAvailable sinceJune 14, 2012AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-Renilla cDNA
Plasmid#29783DepositorInsertRenilla
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pICE-RNaseHI-D10R-E48R-NLS-mCherry
Plasmid#60367PurposePlasmid for expression of an inactive mutant of bacterial RNase HI tagged with NLS-mCherry that can be used to detect R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertRNase HI-D10R-E48R
UseTagsNLS from SV40 T antigen and mCherryExpressionMammalianMutationD10R+E48R; catalyticaly inactivePromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Flag-CDK8-W105M-IRES-mCherry
Plasmid#68857PurposeExpresses Human CDK8 W105M MutantDepositorInsertCyclin-Dependent Kinase 8 (CDK8 Human)
UseLentiviralTagsFlagExpressionMammalianMutationW105MPromoterEF1αAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHR-mCherry-p2A-full length LIC1
Plasmid#74601Purposeexpresses LIC1 in mammalian cells; untagged but cells indicate expression with diffuse mCherryDepositorInsertfull length human dynein light intermediate chain 1 (DYNC1LI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE
Plasmid#20943Purpose3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activationDepositorInsertchannelrhodopsin-2
UseLentiviralTagsmCherryExpressionMammalianMutationH134RPromoterCaMKIIaAvailable sinceJuly 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
paavCAG-pre-mGRASP-mCerulean-2A-nls-mCherry
Plasmid#34911DepositorInsertpresynaptic mGRASP
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human-c-MYC-ER-mouse IRES-mcherry
Plasmid#73570PurposeMammalian retroviral expression of Myc-ERDepositorInsertcMYC (MYC Human)
UseRetroviralTagsIRES-mCherry and Mouse ERExpressionMammalianMutationPromoterMSCVAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.0-TRPV4-p2A-ferritin-p2A-mCherry
Plasmid#74309PurposeTricistronic expression of TRPV4, ferritin, and mCherry in mammalian cellsDepositorInsertTRPV4-p2A-ferritin-p2A-mCherry (Trpv4 Rat, Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(E123T/T159C)-mCherry
Plasmid#35512PurposeAAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123T and T159CPromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry
Plasmid#75470PurposeFlp-dependent Chr2-mCherry encoded in an AAV vector for rAAV production and expression in mammalian cellsDepositorHas ServiceAAV1InsertReversed ChR2(H134R)-mCherry
UseAAV and Synthetic BiologyTagsExpressionMammalianMutationPromoterCAGAvailable sinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLEX-rev-ChR2(H134R)-mCherry
Plasmid#18916PurposeCre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitationDepositorInsertChannelrhodopsin 2-mCherry
UseAAV and Cre/Lox; Adeno-associated virusTagsmCherryExpressionMutationH134RPromoterCAGAvailable sinceJan. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTol2-ziUAS:GPHN.FingR-mCherry-ZFI (IL2RGTC)-KRAB(A)
Plasmid#72639PurposeTol2 based UAS modified with a IL2RGTC binding site upstream of it, driving GPHN-FingR fused to mCherry, IL2RGTC zinc finger, and KRAB(A) repressor domainDepositorInsertUAS upstream of GPHN-FingR with ZFI(IL2RFTC) and KRAB(A)
UseGatewayTagsExpressionMutationStop codon after mCherry- SEE DEPOSITOR COMMENTS …PromoterAvailable sinceApril 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hChR2(C128S/D156A)-mCherry
Plasmid#35502PurposeAAV expression of CaMKII-driven stabilized step function opsin (SSFO) for optogenetic activation.DepositorInserthChR2(C128S/D156A)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationC128S and D156APromoterCaMKIIaAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
MSCV-human NMyc-humanER-IRES-mCherry
Plasmid#73571PurposeMammalian retroviral expression of NMyc-ERDepositorInsertNMyc (MYCN Human)
UseRetroviralTagsHuman ER and IRES-mCherryExpressionMammalianMutationPromoterMSCVAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-TRPM1 cDNA
Plasmid#29782DepositorInsertTRPM1 (TRPM1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
PLKO-mcherry-luc-puro-miR-211
Plasmid#29784DepositorInsertmiR-211
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJune 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSCV-miR30_shBrd9_1061-PGK-NeoR-IRES-mCherry
Plasmid#75132PurposeRetroviral expression plasmid encoding shBrd9_1061 (targeting murine Brd9)DepositorInsertshBrd9_1061
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only