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Addgene
Showing: 1261 - 1280 of 14048 results

  1. 371-C

    Plasmid
    #97029
    Purpose
    His6-MBP-N10-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    His6 tag, MBP domain, N10 linker, and TEV cleavag…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    97029-plasmid-map-sequence-id-182500

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  2. 371-GFP

    Plasmid
    #97030
    Purpose
    His6-GFP-tev-yORF
    Depositor
    Chris Jeans
    Type
    Empty backbone
    Use
    Tags
    GFP fusion, His6 tag, and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    97030-plasmid-map-sequence-id-182506

    Enlarge

  3. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

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  4. MSNA-c007

    Plasmid
    #175467
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-D298. Used for NMR studies.
    Depositor
    Opher Gileadi
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175467-plasmid-map-sequence-id-353136

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  5. MSNA-c023

    Plasmid
    #175469
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-E346. Can be biotinylated.
    Depositor
    Opher Gileadi
    Insert
    MSN (MSN Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175469-plasmid-map-sequence-id-353155

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  6. NF2A-c001

    Plasmid
    #175472
    Purpose
    Protein expression in bacterial cells. FERM domain, M1-R338.
    Depositor
    Opher Gileadi
    Insert
    NF2 (NF2 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175472-plasmid-map-sequence-id-353159

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  7. RAB5AA-c006

    Plasmid
    #175476
    Purpose
    Protein expression in bacterial cells. GTPase domain, G15-N184. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    RAB5A (RAB5A Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175476-plasmid-map-sequence-id-353162

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  8. RABGEF1A-c014

    Plasmid
    #175478
    Purpose
    Protein expression in insect cells. Entire CDS, M1-G491. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    RABGEF1 (RABGEF1 Human)
    Use
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175478-plasmid-map-sequence-id-353251

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  9. SNX32A-c014

    Plasmid
    #175486
    Purpose
    Protein expression in bacterial cells. PX domain, S17-M197
    Depositor
    Opher Gileadi
    Insert
    SNX32 (SNX32 Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175486-plasmid-map-sequence-id-353158

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  10. SYKA-c014

    Plasmid
    #175488
    Purpose
    Protein expression in insect cells. Entire CDS, M1-N635. Codon optimised.
    Depositor
    Opher Gileadi
    Insert
    SYK (SYK Human)
    Use
    Tags
    His6-Zbasic-TEV
    Expression
    Insect
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175488-plasmid-map-sequence-id-353252

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  11. pmCer-C16orf59

    Plasmid
    #211422
    Purpose
    mammalian expression of human C16orf59 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    C16orf59 (C16orf59 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211422-plasmid-map-sequence-id-420524

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  12. pAAV-FLEX-C-RG

    Plasmid
    #49101
    Purpose
    Cre dependent Cerulean-E2A-SADB19 rabies glycoprotein expression, under EF1a promoter, in AAV virus
    Depositor
    Troy Margrie
    Insert
    Cerulean-E2A-RG
    Use
    AAV and Cre/Lox
    Tags
    cerulean
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Availability
    Academic Institutions and Nonprofits only
    49101-plasmid-map-sequence-id-91148

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  13. TBXTA-c019

    Plasmid
    #139756
    Purpose
    For protein expression
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Use
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-A214
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    139756-plasmid-map-sequence-id-296514

    Enlarge

  14. pET28-MKH8SUMO

    Plasmid
    #79526
    Purpose
    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    8xHis-Thrombin-SUMO-TEV and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  15. SDCBPA-c000

    Plasmid
    #175480
    Purpose
    Protein expression in bacterial cells. Entire CDS, M1-V288.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175480-plasmid-map-sequence-id-353167

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  16. SDCBPA-c020

    Plasmid
    #175484
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains, G111-V288.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175484-plasmid-map-sequence-id-353208

    Enlarge

  17. STX4AA-c005

    Plasmid
    #175487
    Purpose
    Protein expression in bacterial cells. Coiled-coil domains, M1-L275.
    Depositor
    Opher Gileadi
    Insert
    STX4 (RUNX1T1 Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175487-plasmid-map-sequence-id-353181

    Enlarge

  18. YSCD44A-c002

    Plasmid
    #175493
    Purpose
    Protein expression in bacterial cells. Single chain Fv antibody to human CD44 Link domain. Can be used for crystallography.
    Depositor
    Opher Gileadi
    Insert
    Single chain Fv CD44 antibody (H90)
    Use
    Tags
    3XFLAG-EK-His8 and pelB
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175493-plasmid-map-sequence-id-353166

    Enlarge

  19. pmCer-Mcm5

    Plasmid
    #211391
    Purpose
    mammalian expression of human Mcm5 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    Mcm5 (MCM5 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211391-plasmid-map-sequence-id-420408

    Enlarge

  20. pmCer-DHX9

    Plasmid
    #211408
    Purpose
    mammalian expression of human DHX9 N-terminally fused to mCerulean
    Depositor
    Helmut Pospiech
    Insert
    DHX9 (DHX9 Human)
    Use
    Tags
    mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    211408-plasmid-map-sequence-id-420513

    Enlarge

Showing: 1261 - 1280 of 14048 results