Skip to main content
Addgene
Showing: 1201 - 1220 of 29286 results

  4. MCS-BioID2-HA pBabe-puro

    Plasmid
    #120308
    Purpose
    To fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS is present upstream of BioID2 tag and HA tag
    Depositor
    Kyle Roux
    Type
    Empty backbone
    Use
    Retroviral
    Tags
    -BioID2-HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    120308-plasmid-map-sequence-id-239324

    Enlarge

  10. pBABE puro K-Ras V12

    Plasmid
    #9052
    Purpose
    Retroviral expression of a constitutively active form of K-Ras
    Depositor
    William Hahn
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    G12V. Constitutively active.
    Promoter
    Available since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only
    9052-plasmid-map-sequence-id-219889

    Enlarge

  14. tet-pLKO-sgRNA-puro

    Plasmid
    #104321
    Purpose
    3rd generation lentiviral plasmid for inducible expression of sgRNA; derived from tet-pLKO-puro; puromycin selection. See manual for detailed protocols.
    Depositor
    Nathanael Gray
    Article
    Huang et al Elife. 2017 Sep 19;6. pii: e26693. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    H1/TO
    Available since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    104321-plasmid-map-sequence-id-323839

    Enlarge

  15. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

    Enlarge

  20. PB-TO-NGN2-EMX1 puro-BFP

    Plasmid
    #182312
    Purpose
    Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 and EMX1 expression, puromycin selection, BFP
    Depositor
    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward
    Inserts
    hNGN2-P2A-EMX1 (NEUROG2, EMX1 Human)
    puro-T2A-mycNLS-mTagBFP2
    Use
    Tags
    T2A-mycNLS-mTagBFP2
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and TRE3G
    Available since
    April 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    182312-plasmid-map-sequence-id-359526

    Enlarge

Showing: 1201 - 1220 of 29286 results