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Showing: 101 - 120 of 18970 results

  2. pSECRETS-A

    Plasmid
    #196986
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: bacterial expression Cas9 in the presence of anhydrotetracycline (aTc).
    Depositor
    Eric Josephs
    Article
    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1
    Insert
    S. pyogenes Cas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pltetO1
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    196986-plasmid-map-sequence-id-396953

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  6. pRSETA_crE6G2

    Plasmid
    #172929
    Purpose
    Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices, and a 6AA GSG domain
    Depositor
    Arnold Boersma
    Article
    Liu et al Biophys J. 2017 May 9;112(9):1929-1939.
    Insert
    CrowdingSensorE6G2
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Val317Gly, Met316Gly
    Promoter
    T7
    Available since
    Aug. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    172929-plasmid-map-sequence-id-339474

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  10. pcDNA_DiCre2_59.F2

    Plasmid
    #60209
    Purpose
    Expresses a fusion protein of FKBP12(F36V)-linker peptide 2- Cre(19-59)
    Depositor
    Jean-Paul Herman
    Insert
    Cre recombinase (CD207 Human, Synthetic, Bacteriophage P1)
    Use
    Tags
    FKBP12(F36V) and Nuclear localisation signal
    Expression
    Mammalian
    Mutation
    FKBP12 has the F36V mutation; amino acids 19-59 o…
    Promoter
    CMV
    Available since
    Jan. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    60209-plasmid-map-sequence-id-96295

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  18. Th-iCre

    Plasmid
    #159459
    Purpose
    Targeting vector to TH gene locus for induction of P2A-fused iCre, with PGK-puroR drug resistant cassette flanked by FRT sequence.
    Depositor
    Yuejun Chen
    Article
    Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-
    Insert
    P2A-iCre
    Use
    Cre/Lox
    Tags
    P2A
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous TH
    Available since
    Dec. 8, 2020
    Availability
    Academic Institutions and Nonprofits only
    159459-plasmid-map-sequence-id-319220

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  19. Bxb1Donor_G102H_ADRB2_LucReporter

    Plasmid
    #129794
    Purpose
    encodes a donor vector for recombination with the Bxb1 recombinase with variant G102H of the Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene
    Depositor
    Sriram Kosuri
    Article
    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1
    Insert
    ADRB2 (ADRB2 Human)
    Use
    Synthetic Biology
    Tags
    3x FLAG
    Expression
    Mammalian
    Mutation
    G102H
    Promoter
    N/A
    Available since
    Sept. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    129794-plasmid-map-sequence-id-255134

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Showing: 101 - 120 of 18970 results