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Showing: 151 - 180 of 158672 results

  3. pDisCoTune

    Plasmid
    #173713
    Purpose
    Promotes expression of disulfide-rich protein in E. coli T7 expression system. The plasmid encodes expression of the folding factors Erv1p and hPDI controlled by the Ptac promoter.
    Depositor
    Morten Norholm
    Article
    Bertelsen et al Microb Biotechnol. 2021 Nov;14(6):2566-2
    Inserts
    ERV1 (ERV1 Budding Yeast)
    PDI
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    173713-plasmid-map-sequence-id-342928

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  4. pHIV-Luc-ZsGreen

    Plasmid
    #39196
    Depositor
    Bryan Welm
    Inserts
    Firefly Luciferase Luc2P
    ZsGreen
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1-alpha and EF1-alpha
    Available since
    Oct. 30, 2012
    Availability
    Academic Institutions and Nonprofits only
    39196-plasmid-map-sequence-id-335121

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  7. pSNAP-tag (m) Vector

    Plasmid
    #101135
    Purpose
    Expression of protein of interest with SNAP-Tag in Mammalian cells
    Depositor
    New England Biolabs, Ana Egana
    Type
    Empty backbone
    Use
    Tags
    SNAP-tag (SNAP26m)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 27, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101135-plasmid-map-sequence-id-194101

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  10. pdCas9-bacteria

    Plasmid
    #44249
    Purpose
    aTc-inducible expression of a catalytically inactive bacterial Cas9 (S. pyogenes) for bacterial gene knockdown
    Depositor
    Stanley Qi
    Article
    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1
    Insert
    dCas9 (bacteria)
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    D10A H840A (catalytically inactive)
    Promoter
    pLtetO-1
    Available since
    April 11, 2013
    Availability
    Academic Institutions and Nonprofits only
    44249-plasmid-map-sequence-id-269588

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  14. pGEM4Z-T7-5'UTR-EGFP-3'UTR-A64

    Plasmid
    #203348
    Purpose
    Template for T7 in vitro transcription of EGFP mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail
    Depositor
    Christopher Grigsby, Molly Stevens
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Template for t7-mediated in vitro transcription o…
    Tags
    Flanked by 5' and 3' UTRs from Xenopus …
    Expression
    Mutation
    Promoter
    T7
    Available since
    June 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    203348-plasmid-map-sequence-id-399143

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  16. pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (AAV1)

    Viral Prep
    #100848-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 (#100848). In addition to the viral particles, you will also receive purified pAAV.Syn.NES.jRCaMP1a.WPRE.SV40 plasmid DNA. Syn-driven jRCaMP1a calcium sensor. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project, Douglas Kim
    Article
    Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:
    Promoter
    Syn
    Tags
    None
    Available since
    March 16, 2018
    Availability
    Academic Institutions and Nonprofits only

  18. pGTM_YR375_SUMO_Protease_001

    Plasmid
    #190063
    Purpose
    Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion
    Depositor
    Gaetano Montelione
    Article
    Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi
    Insert
    ULP1_YEAST (ULP1 Budding Yeast)
    Use
    Tags
    8X His-MBP (Maltose Binding Protein)
    Expression
    Bacterial
    Mutation
    Construct contains residues 347 – 621
    Promoter
    T7 lac
    Available since
    Jan. 30, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    190063-plasmid-map-sequence-id-370664

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  19. pCMV-PEmax-P2A-hMLH1dn

    Plasmid
    #174828
    Purpose
    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)
    Depositor
    David Liu
    Article
    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.
    Insert
    PEmax-P2A-hMLH1dn
    Use
    Tags
    SV40 bpNLS and c-Myc NLS
    Expression
    Mammalian
    Mutation
    Detailed in manuscript; deletion of residues 754-…
    Promoter
    CMV
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    174828-plasmid-map-sequence-id-357934

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  26. LentiGuide Puro-P2A-EGFP

    Plasmid
    #137729
    Purpose
    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).
    Depositor
    Fredrik Wermeling
    Article
    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p
    Type
    Empty backbone
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Tags
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    137729-plasmid-map-sequence-id-272646

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  28. pAAV-CKIIa-stGtACR2-FusionRed (AAV1)

    Viral Prep
    #105669-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CKIIa-stGtACR2-FusionRed (#105669). In addition to the viral particles, you will also receive purified pAAV-CKIIa-stGtACR2-FusionRed plasmid DNA. CaMKIIa-driven, soma-targeted anion-conducting channelrhodopsin fused to FusionRed for optogenetic inhibition. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Ofer Yizhar
    Article
    Mahn et al Nat Commun. 2018 Oct 8;9(1):4125. doi: 1
    Promoter
    CaMKII
    Tags
    FusionRed
    Available since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 151 - 180 of 158672 results