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Plasmid#89088Purposemammalian expression of human androgen receptor with deletion of 120-472 amino acid residuesDepositorInserthAR-120-472 N-term deletion (AR Human)
UseTagsExpressionMammalianMutationhAR-120-472PromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-1574/+120
Plasmid#35539DepositorUseLuciferaseTagsluciferaseExpressionMutationPromoternoneAvailable sinceJune 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CARMA2cl-FLAG
Plasmid#97077PurposeExpresses CARMA2cl/CARD14 isoform 2 in mammalian cellsDepositorInsertCARD14 isoform 2 (CARD14 Human)
UseTagsFLAGExpressionMammalianMutationnonePromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pF708-pET28a-Hs-delta38-METTL20-NHis wt
Plasmid#85109Purposeexpression of recombinant human mature (delta38) METTL20 wt with N-terminal 6xHis-tagDepositorInserthuman mature (delta38) METTL20 (METTL20 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CMV.SV40.THBS1-HA-KDEL.SV40(polyA)
Plasmid#156164PurposeExpresses murine Thrombospondin-1 (THBS1) protein with HA-tag at C-terminus and KDEL (ER retention) sequenceDepositorInsertThrombospondin-1 (Thbs1 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationTHBS1 with addition of ER-retention sequence KDELPromoterCMVAvailable sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-mAPPLE
Plasmid#60696Purposeretroviral expression of rat Cx43 fused to mAPPLEDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsmAPPLEExpressionMammalianMutationPromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 hCortKD-Flcortmut3YF EGFP
Plasmid#187263PurposeLentiviral expression of shRNA targeting Cortactin + reconstitution of Cortactin3YF mutantDepositorInsertCortactin (CTTN Human)
UseLentiviralTagsEGFPExpressionMutation3YF (Y412, Y470, Y486)PromoterU6, 5'LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR4-Dact1(1250-1601)
Plasmid#52046PurposeTo generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.DepositorInsertDact1(1250-1601) (Dact1 Mouse)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
pYPQ239-RR
Plasmid#108862PurposeFnCpf1 Gateway entry plasmid with N623R and K687R mutationsDepositorInsertFnCpf1-RR (FnCpf1 with N623R and K687R mutations)
UseCRISPR; Gateway compatible cpf1 entry cloneTagsNLSExpressionPlantMutationN623R and K687R, Cpf1 is rice codon optimizedPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-HA-GATA3 C1(259-443)
Plasmid#89832PurposeExpress GATA3 C1 in mammalian cellsDepositorInsertGata3 (Gata3 Mouse)
UseRetroviralTagsHAExpressionMutationC1 (AA 259-443)PromoterCMVAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJO427
Plasmid#111139PurposeURT-type vector for Glu-mIκBα expression in Mammalian cellsDepositorInsertflag-lacZ25-119-DHFR-UbK48R-Glu51-mIκBα (Ikbkb Mouse)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S279D
Plasmid#60707Purposeretroviral expression of rat Cx43 S279D mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS279DPromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S282A
Plasmid#60709Purposeretroviral expression of rat Cx43 S282A mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS282APromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S282D
Plasmid#60711Purposeretroviral expression of rat Cx43 S282D mutantDepositorInsertCx43 (Gja1 Rat)
UseRetroviralTagsExpressionMammalianMutationS282DPromoterLTRAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRR-C421-Y2404F
Plasmid#182846Purposemutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG inductionDepositorInsertTRR 2011-2431 (trr Fly)
UseTagsExpressionBacterialMutationmutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…PromoterAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13-IDR-YFP-NLS
Plasmid#145277PurposeMammalian Expression of Nuclear HOXD13 IDR-YFP with SV40 NLSDepositorInsertHOXD13-IDR (HOXD13 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xNLS-NLP-cMyc-cMyc enAspCas12a
Plasmid#182127PurposepET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteriaDepositorInsert3xNLS-NLP-cMyc-cMyc enAspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a RUVBL2 Full Length
Plasmid#159142PurposeExpresssion and protein purification of full length RUVBL2DepositorInsertRUVBL2 (RUVBL2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCR3-vsv-GCN4-dCEN-INCENP
Plasmid#108510Purposeexpression of vsv-GCN4-dCEN-INCENPDepositorInsertGCN4-dCEN-INCENP (INCENP Human)
UseTagsVSVExpressionMammalianMutationreplace CEN box for GCN4 dimerazation domainPromoterCMVAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-CRY2-P14P5K
Plasmid#79569Purposeexpression of GFP-tagged CRY2PHR fused to iSH2 domain of mouse Pip5k1cDepositorInsertPip5k1c (Pip5k1c Mouse)
UseTagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationR445E and R446E; truncation of amino acids 636-66…PromoterCMVAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only