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Plasmid#60814Purposemammalian expression vectorDepositorInsert53BP1 (TP53BP1 Human)
UseTagseGFP-NLS (SV40)ExpressionMammalianMutationPromoterAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
HiBiT-FZD7
Plasmid#195845PurposeExpresses human FZD7 with an N-terminal HiBiTDepositorInsertFZD7 (FZD7 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIRES2-EGFP-p53 tethered
Plasmid#49245Purposeexpresses two human WT p53s tethered together and also expresses EGFP in mammalian cellsDepositorInsertp53-p53 (TP53 Human)
UseTagsIRES-EGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pH3R-mCherry-N1
Plasmid#84327PurposeHistamine-3 receptor tagged with mCherryDepositorInsertH3R-mCherry (HRH3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)10-(SIM)5
Plasmid#126866PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.DepositorInsert10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pH4R-mCherry-N1
Plasmid#84328PurposeHistamine-4 receptor tagged with mCherryDepositorInsertH4R-mCherry (HRH4 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA-32Asp8
Plasmid#102617PurposeExpression of human RPA with aspartic acid substituted phosphorylation domainDepositorUseTagsExpressionBacterialMutationwith aspartic acid substituted phosphorylation do…PromoterAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-ihSyn1-DIO-tTA
Plasmid#99121PurposeAn AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTADepositorHas ServiceAAV1InsertDIO-tTA
UseAAVTagsExpressionMammalianMutation*(see below)PromoterihSyn1Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
Sniper-Cas9
Plasmid#138559PurposeExpresses human codon-optimized Sniper-SpCas9 and blasticidin resistance: EFS promoter-Sniper-Cas9-NLS-FLAG-P2A-BSDDepositorInsertSniper-Cas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationF539S, M763I, K890NPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
HSC1-HS4-D4Z4c-GiP
Plasmid#58541PurposeDerived from HSC1-GiP where 2 copies of the HS4 insulator core inside the 3'LTR and the D4Z4-c insulator, are combined upstream of the EF1 alpha promoterDepositorInsertsEnhanced Green Fluorescence Protein, Puromycin resistance gene
D4Z4 repeat, partial
UseRetroviralTagsExpressionMammalianMutationPromoterHuman EF1-alphaAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJM31
Plasmid#52986PurposeA secreted Annexin V (sAnxV::GFP) Phosphatidylserine sensorDepositorInsertAnnexin V (ANXA5 Human)
UseTagsGFP (w/ introns) and signal sequenceExpressionWormMutationPromoterhsp-16.41Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGM190
Plasmid#69614Purposeinducible gene expression in StreptomycesDepositorInsertsaphII
tsr
rep
sso
TipA promoter
dso
UseStreptomyces- e. coli shuttle vectorTagsExpressionBacterialMutationPromoterAvailable sinceJan. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Dual-iCas9-EFS
Plasmid#167501PurposeExpression of dual inducible Cas9 with EFS promoterDepositorInsertCas9
UseCRISPR, Lentiviral, and LuciferaseTagsFLAGExpressionMammalianMutationPromoterEFSAvailable sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
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HiBiT-FZD8
Plasmid#195846PurposeExpresses human FZD8 with an N-terminal HiBiTDepositorInsertFZD8 (FZD8 Human)
UseTagsHiBiTExpressionMammalianMutationPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYPQ-dpcoCas9-Act3.0
Plasmid#158408PurposeCRISPR-Act3.0 system containing dpcoCas9-VP64 fusion protein, T2A linked MS2-SunTag fusion protein, and ScFv-sfGFP-2xTAD activator.DepositorInsertdpcoCas9-VP64-T2A-MS2-SunTag-rbcS-E9t-ter-ZmUbi-scFv-sfGFP-2xTAD-GB1-NOS-ter
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMXs-Gfi1b
Plasmid#131611Purposeexpression of mouse Gfi1b in mammalian cellsDepositorInsertGfi1b (Gfi1b Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD3
Plasmid#195841PurposeExpresses human FZD3 with an N-terminal HiBiT; contains I292V and V495IDepositorInsertFZD3 (FZD3 Human)
UseTagsHiBiTExpressionMammalianMutationI292V and V495IPromoterCMVAvailable sinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMZ827
Plasmid#155321PurposeConstitutive expression of E-PIF6(1-100) in plantsDepositorInsertPCaMV35S-E-PIF6(1-100)-NLS-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF394
Plasmid#155333PurposeExpression of PULSE-controlled LFY-VP16-2A-RLucDepositorInsert(etr)8-(C120)5-PhCMVmin-LFY-VP16-NLS-2A-RLuc-TSV40
UseSynthetic Biology; Eukaryotic expressionTagsExpressionMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only