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Addgene
Showing: 11761 - 11780 of 147719 results
  1. RGD-I27-CC

    Plasmid
    #85393
    Purpose
    MTFM mechanosensor for integrin. Integrin binding domain fused to titan immunoglobulin domain (I27) with two cysteines for immobilization and site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Insert
    RGD motif for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  2. FN(9-10)-I27-CC

    Plasmid
    #85394
    Purpose
    MTFM mechanosensor for integrin. Fibronectin domains 9&10 fused to titan immunoglobulin domain (I27) with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Insert
    Fibronectin (FN) domains 9 & 10 for integrin binding fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  3. FN(9-10, RGE)-I27-CC

    Plasmid
    #85396
    Purpose
    MTFM mechanosensor for integrin. Fibronectin domains 9&10 with RGE mutation fused to titan I27 domain with two cysteines for immobilization, site for p-azidophenylalanine incorporation & Cy3 labeling
    Depositor
    Insert
    Fibronectin (FN) domains 9&10 containing RGE mutation fused to titin immunoglobulin domain (I27), containing a TAG (amber) codon
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    GRGDS motif mutated to GRGES
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  4. pAAV hSyn1 bPAC cMyc T2A tDimer

    Plasmid
    #85397
    Purpose
    humanized photoactivated adenylyl cyclase with cMyc Tag driven by neuron-specific promoter, plus red fluorescent protein
    Depositor
    Inserts
    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    Use
    AAV
    Tags
    cMyc Tag
    Expression
    Mammalian
    Mutation
    Promoter
    human synapsin-1 and ribosomal skip sequence T2A
    Availability
    Academic Institutions and Nonprofits only
  5. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

    Plasmid
    #85398
    Purpose
    humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein
    Depositor
    Inserts
    humanized photoactivated adenylyl cyclase
    red fluorescent protein
    Use
    AAV
    Tags
    cMyc Tag
    Expression
    Mammalian
    Mutation
    S27A
    Promoter
    human Synapsin1 promotor and ribosomal skip seque…
    Availability
    Academic Institutions and Nonprofits only
  6. Lenti-iCas9-neo

    Plasmid
    #85400
    Purpose
    Lentiviral vector encoding a doxycycline inducible EGFP reporter downstream of FLAG-tagged spCas9, separated by a P2A self-cleavage sequence
    Depositor
    Insert
    Flag-iCas9-P2A-GFP
    Use
    Lentiviral
    Tags
    Flag-iCas9-P2A-GFP
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  7. Lenti-multi-Guide

    Plasmid
    #85401
    Purpose
    Lentiviral vector for the delivery of multiple sgRNAs targeting different genes in combination with inducible Cas9 expresssion by pLenti-iCas9-neo
    Depositor
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. pMXs-E55A-ATPIF1

    Plasmid
    #85404
    Purpose
    Retroviral vector expressing human ATPIF1 with E55A mutation, which abrogates the interaction between ATPIF1 and the ATP synthase
    Depositor
    Insert
    ATPIF1 (ATP5IF1 Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    E55A point mutation abrogating the ability of ATP…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
Showing: 11761 - 11780 of 147719 results