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pAAV hSyn FLEx-FRT hM4Di-mCherry

Plasmid

#161576

Purpose

Expression of hM4Di-mCherry in a Flp-dependent fashion

Depositor

Kevin Beier, Robert Malenka

Article

Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

Insert

hM4Di-mCherry

Use

AAV

Tags

Expression

Mutation

NA

Promoter

Available since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304-CAR-V5

Plasmid

#82723

Purpose

Expresses V5 tagged coxsackievirus and adenovirus receptor (CAR).

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

coxsackievirus and adenovirus receptor (CXADR Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
HPV16 E7 TCR

Plasmid

#122728

Purpose

Express HPV16 E7 TCR

Depositor

Christian Hinrichs

Article

Jin et al JCI Insight. 2018 Apr 19;3(8). pii: 9948

Insert

HPV16 E7 TCR

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-(SIM)3

Plasmid

#126955

Purpose

Bacterial expression plasmid for eGFP-(SIM)3 client to assess SUMO-SIM scaffolds.

Depositor

Michael Rosen

Article

Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

Insert

EGFP-SUMO interacting motif (SIM) from PIASx (PIAS2 Human)

Use

Tags

eGFP

Expression

Bacterial

Mutation

Promoter

unknown

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

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UbcH5B

Plasmid

#60443

Purpose

Express E2 UbcH5B in E. Coli

Depositor

Cynthia Wolberger

Article

Wiener et al Nat Struct Mol Biol. 2013 Sep;20(9):1033

Insert

UbcH5B (UBE2D2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-14-3-3 epsilon GST

Plasmid

#13279

Depositor

Michael Yaffe

Article

Yaffe et al Cell. 1997 Dec 26. 91(7):961-71.

Insert

14-3-3 epsilon (Ywhae Rat)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

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4QQD

Plasmid

#61947

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

UHRF1:SDC130-H02:C39674 (UHRF1 Human)

Use

Tags

6xHis and MHHHHHHSSGRENLYFQG

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available since

March 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAAV-Ef1a-DO-hChR2(H134R)-mCherry-WPRE-pA

Plasmid

#37082

Purpose

Cre-dependent expression of hChR2(H134R) for optogenetic excitation

Depositor

Bernardo Sabatini

Article

Kozorovitskiy et al Nature. 2012 May 13;485(7400):646-50. do

Insert

hChr2(H134R)-mCherry

Use

AAV and Cre/Lox; Cre-off

Tags

mCherry

Expression

Mutation

Promoter

EF1a

Available since

June 14, 2012

Availability

Academic Institutions and Nonprofits only

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DDX5 WT V5

Plasmid

#199576

Purpose

Expresses WT DDX5 cloned into pLenti puro

Depositor

Michael Kastan

Article

McCann et al Radiat Res. 2023 Apr 1;199(4):406-421. d

Insert

DDX5 (DDX5 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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mOrange pHis-//

Plasmid

#53304

Purpose

Empty vector to express N-terminal His-mOrange-tagged fusion protein.

Depositor

John Lis

Article

Tome et al Nat Methods. 2014 Jun;11(6):683-8. doi:

Type

Empty backbone

Use

Tags

His and mOrange

Expression

Bacterial

Mutation

Promoter

Available since

June 4, 2014

Availability

Academic Institutions and Nonprofits only

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pMRBAD-Z-CGFP

Plasmid

#52735

Purpose

L-(+)-arabinose-inducible expression of Z-CGFP positive control for in vivo split GFP assembly assay

Depositor

Lynne Regan

Article

Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

Insert

Z+CGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD

Available since

May 7, 2014

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ytdTomato

Plasmid

#118456

Purpose

Overexpression of yeast codon-optimized tdTomato

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ytdTomato

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PPP1R12A wt

Plasmid

#31658

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12A (PPP1R12A Human)

Use

Tags

M2

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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Ai148 (TIT2L-GC6f-ICL-tTA2) targeting vector

Plasmid

#114428

Purpose

Target a Cre-dependent GCaMP6f expression and a tTA2 cassette into the mouse TIGRE locus

Depositor

Hongkui Zeng

Article

Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

Insert

GCaMP6f

Use

Cre/Lox and Mouse Targeting

Tags

Expression

Mutation

Promoter

TRE2, CAG

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_WT

Plasmid

#83482

Purpose

Bacterial expression of WT L. buccalis C2c2 CRISPR effector.

Depositor

Jennifer Doudna

Article

East-Seletsky et al Nature. 2016 Sep 26. doi: 10.1038/nature

Insert

Lbu_C2c2_WT

Use

CRISPR

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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BRAF in pENTR

Plasmid

#216605

Purpose

gateway cloning donor vector containing BRAF

Depositor

Christopher Loewen, Douglas Allan

Insert

BRAF (BRAF Human)

Use

Gateway entry plasmid

Tags

Expression

Mutation

Promoter

Available since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-puro-CMV-VC3AI

Plasmid

#78907

Purpose

Indicator of caspase-3-like protease activity

Depositor

Binghui Li

Article

Zhang et al Nat Commun. 2013;4:2157. doi: 10.1038/nc

Insert

VC3AI

Use

Lentiviral

Tags

HA and Myc

Expression

Mutation

S320A, deletion of AA1-200

Promoter

CMV

Available since

June 29, 2016

Availability

Academic Institutions and Nonprofits only

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pC014 - LwCas13a-msfGFP

Plasmid

#91902

Purpose

Expresses active LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR

Tags

NLS and msfGFP-NLS-3xHA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C9orf72

Plasmid

#83437

Purpose

Expresses GFP-C9orf72 in mammalian cells

Depositor

Shawn Ferguson

Article

Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

Insert

C9orf72 (C9orf72 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6MFV SLC10A1

Plasmid

#194130

Purpose

Expresses NTCP (SLC10A1) protein in mammalian cells

Depositor

Jun Liu

Insert

SLC10A1 (SLC10A1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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