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Plasmid#220848PurposeLentiviral vector expressing ZFcharm Kv2 fused to ZF3 and MmZFP_81187 via split Npu inteins; perfect match ZF3 binding site located upstream of pEFSDepositorInsertZFcharm Kv2
UseLentiviralTagsP2A-mCherryExpressionMammalianMutationN/APromoterEFSAvailable sinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
Plasmid#207863PurposeAAV genome encoding C-terminal PE6d and U6 expression cassettes for Dnmt1 PE3 pegRNA and ngRNADepositorInsertNpuC-Cterm-PE6d-dualU6-Dnmt1-PE3-loxP
UseAAVTagsExpressionMutationSee ManuscriptPromoterCbhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP NLS Beta-Actin S14C
Plasmid#60614PurposeEncodes YFP and NLS tagged beta-actin with the S14C polymerization mutationDepositorInsertBeta-actin (ACTB Human)
UseTagsEYFP and NLSExpressionMammalianMutationS14CPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO-SCN8A-Variant-1-IRES-mScarlet
Plasmid#162280PurposeEukaryotic expression of human SCN8A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.DepositorInsertSCN8A Variant 1, stabilized (SCN8A Human)
UseTagsIRES-mScarletExpressionMammalianMutationModified human HSPA5 intron 4 inserted after bp23…PromoterCMVAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pinducer 20 DN-KASHΔPPPL
Plasmid#129280PurposeTet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPLDepositorInsertSYNE1 (SYNE1 Human)
UseLentiviralTagsmcherryExpressionMutationthe last 4 amino acids (PPPL) of the KASH domain …PromoterCMVAvailable sinceAug. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro
Plasmid#183410PurposepiggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cellsDepositorInsertKRAB-dCas9-ecDHFR
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-Coff/Fon-BFP
Plasmid#137131PurposeIntersectional viral expression of BFP in cells expressing Flp AND NOT CreDepositorHas ServiceAAV8InsertCoff/Fon-BFP
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationn/aPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-mCherry-Fc(DAPA)-AviTag-6xHis
Plasmid#156581PurposeMammalian expression of mCherry fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertmCherry
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP7f
Plasmid#104483PurposeMammalian expression of ultrasensitive protein calcium sensorDepositorInsertjGCaMP7f
UseTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-CPAP
Plasmid#136815PurposeMammalian expression of the centrosomal protein CENPJ N-terminally fused to SNAP-tagDepositorInsertSNAP-CENPJ (CENPJ Human, Synthetic)
UseTagsFLAG-tag / His-tagExpressionMammalianMutationPromoterCMV-TetO2Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRCN0000338399
Plasmid#78156PurposeshXPO1 targeting CDSDepositorInsertXPO1 (XPO1 Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterhU6 and hU6Available sinceJune 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV2 for Split-PE, gRNAs + MMLV-RT(dRH)-P2A-eGFP (PKC1002)
Plasmid#190112PurposeAAV2 for expression of pegRNA and ngRNA (HEKs3, CTT insertion) + MMLV-RT(dRH)-P2A-eGFPDepositorInsertsMMLV-RT(dRH)
U6-pegRNA(HEKs3, CTT ins)-H1-ngRNA(HEK s3)
UseAAVTagsbpNLS and bpNLS-P2A-eGFPExpressionMutationmutations from RT in PE2 and truncation of RNAse …PromoterEFS and U6, H1Available sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_SPTLC1_C133W
Plasmid#181921Purpose3rd generation, Inducible lentiviral plasmid for expression of human mutant SPTLC1 C133WDepositorInsertHuman serine palmitoyltransferase, long chain base subunit 1 (SPTLC1) mutant C133W (SPTLC1 Human)
UseLentiviral; Mammalian expression, , doxycycline i…TagsExpressionMammalianMutationcysteine at position 133 of the coding region of …Promotertight TRE promoterAvailable sinceMay 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-Cas13Y.1-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA
Plasmid#171381PurposeExpression vector for encoding a human codon-optimized Cas13Y.1 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized Cas13Y.1
UseTags3xFLAGExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG1_M18
Plasmid#82357PurposeExpression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.DepositorInsertmouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
UseTagsnoneExpressionMammalianMutationnonePromoterhEF1-HTLV promAvailable sinceSept. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV_Syn_Xph20_eGFP_CCR5TC
Plasmid#187444PurposeEncodes a specific PSD-95 binder (Xph20) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domainDepositorInsertXph20
UseAAVTagseGFPExpressionMammalianMutationPromoterSynapsinAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-AR Q48
Plasmid#86428PurposeFluorescent human androgen receptor (fused to EGFP) with expanded polyglutamine regionDepositorInsertAndrogen receptor (AR) (AR Human)
UseTagsEGFPExpressionMammalianMutationPoly-glutamine expansion, G130DPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-N-Flag-Caspase-1
Plasmid#75128PurposeExpresses N-terminal Flag-tagged Caspase-1 in mammalian cellsDepositorInsertcaspase 1 (Casp1 Mouse)
UseTagsFlagExpressionMammalianMutationP341S in Caspase-1 (please see depositor comments…PromoterCMVAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX304 ERG
Plasmid#118625PurposeOverexpresses ERG proteinDepositorInsertERG (33-479) (ERG Human)
UseLentiviralTagsNone and noneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-Con/Fon-GCaMP6M
Plasmid#137119PurposeIntersectional viral expression of GCaMP6M in cells expressing both Cre AND FlpDepositorHas ServiceAAV8InsertCon/Fon-GCaMP6M
UseAAV, Cre/Lox, and Synthetic Biology ; Flp/frtTagsExpressionMutationRemoved RSET tagPromoterEF1aAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only