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Plasmid#224398PurposeFor expression of the type I CBASS system encoded by Escherichia coli K71-77DepositorInsertPhospholipase_EcCdnE_DisA-like
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Escherichia_coli_RU1_BHI6
Plasmid#224397PurposeFor expression of the type I CBASS system encoded by Escherichia coli RU1 BHI6DepositorInsertCap15_EcCdnE
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD02_CBASS_Xanthomonas_phaseoli
Plasmid#224392PurposeFor expression of the type I CBASS system encoded by Xanthomonas phaseoliDepositorInsertXpCdnC_Endonuclease
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28Man
Plasmid#203399Purposeexo-beta mannosidase useful in enzymatic biotransformations of glycans and in coupled assays of various carbohydrate processing enzymesDepositorInsertman2A
UseTagsH6ExpressionBacterialMutationNonePromoterT7Available sinceAug. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-IFT57
Plasmid#218728PurposeExpresses N-terminally EGFP-tagged IFT57 in mammalian cellsDepositorInsertIFT57 (IFT57 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
RepairTemplate_NIPBL_mEGFP_mAID
Plasmid#217664PurposeRepair template to endogenously tag NIPBL with mEGFP-mAID TagDepositorInsertNIPBL_mEGFP_mAID (NIPBL Human)
UseTagsmAID and meGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSF1b-His8-TEV-PI31-1-151
Plasmid#202593PurposeBacterial expression of HsPI31-1-151DepositorInsertPI31 (PSMF1 Human)
UseTags8xHis, TEVExpressionBacterialMutationaa 1-151 onlyPromoterAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Cas9-hGem_gRNA2
Plasmid#217659Purposeexpresses Cas9-hGem and guideRNA targeting the human AAVS1 safe harbour locusDepositorInsertsgRNA2 targeting the human AAVS1 safe harbour locus
UseCRISPRTagsExpressionBacterial and MammalianMutationPromoterU6Available sinceJuly 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-Flag-Miwi-EGFP
Plasmid#212575PurposeLentiviral expression of Flag-tagged mouse Miwi in mammalian cellsDepositorInsertMiwi (Piwil1 Mouse)
UseLentiviralTags5x Flag and SNAP tagExpressionMammalianMutationPromoterSFFVAvailable sinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF702
Plasmid#209650PurposeSuicide plasmid carrying a neomycin resistance marker flanked by homologous regions of the Mth60-fimbria encoding operon of Methanothermobacter thermautotrophicusDepositorInsertsDownstream homologous region
thermostable neomycin phosphotransferase gene
Upstream homologous region
UseTagsExpressionMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
hPRL2_1-163
Plasmid#209794PurposeBacterial expression of human PRL-2 without farnesylation motifDepositorInsertPRL-2 (PTP4A2 Human)
UseTags6xHisExpressionBacterialMutationaa 1-163 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
FKBP12-CB2mutx2-mTagBFP2-HAx2
Plasmid#207147PurposeTandem mutant calcium-buffer with rapalog dimerizerDepositorInsertCalbindin-2 (CALB2 Human)
UseTagsFKBP12, HA, and mTagBFP2ExpressionMammalianMutationE40Q, E87Q, E131Q, E175Q, E219QPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF018
Plasmid#155325PurposeConstitutive expression of KRAB-EL222 in plantsDepositorInsertPCaMV35S-NLS-KRAB-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pROF050
Plasmid#155327PurposeConstitutive expression of BRD-EL222 in plantsDepositorInsertPCaMV35S-BRD-NLS-EL222-Tnos
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-RnCENP-O-GFP
Plasmid#205179PurposeExpresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorInsertCENP-O (Cenpo Rat)
UseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationWild type rat CENP-OPromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-hSynapsin-Nluc-EPAC-VV
Plasmid#204846PurposeProduce lentivirus encoding Nluc-EPAC sensor in neuronal cell typeDepositorInsertNluc-EPAC sensor
UseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-G132EL133EP134RT137R-dsRNAres_Y
Plasmid#148109PurposeInsect Expression of DmPAN2-G132EL133E134RT137R-dsRNAresDepositorInsertDmPAN2-G132EL133E134RT137R-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-del975-1031GSSG-dsRNAres_Y
Plasmid#148069PurposeInsect Expression of DmPAN2-del975-1031GSSG-dsRNAresDepositorInsertDmPAN2-del975-1031GSSG-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F99E-dsRNAres_Y
Plasmid#148072PurposeInsect Expression of DmPAN2-F99E-dsRNAresDepositorInsertDmPAN2-F99E-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-F99EG132EL133E-dsRNAres_Y
Plasmid#148073PurposeInsect Expression of DmPAN2-F99EG132EL133E-dsRNAresDepositorInsertDmPAN2-F99EG132EL133E-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only