-
Viral Prep#179460-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-EF1a-DIO-JEDI-2P-WPRE (#179460). In addition to the viral particles, you will also receive purified pAAV-EF1a-DIO-JEDI-2P-WPRE plasmid DNA. EF1a-driven, Cre-dependent expression of voltage indicator JEDI-2P. These AAV preparations are suitable purity for injection into animals.DepositorPromoterEF1aTagscEGFP (Cre-dependent)Available sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only
-
TALEN-mROSA26 ELD
Plasmid#60026PurposeExpression of a TALEN protein specific for mouse ROSA26 locus.DepositorInsertTALEN-ROSA26
UseTALENTagsExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
GZnP3 in pcDNA3.1+
Plasmid#161738PurposeCytosolic fluorescent reporter for Zn²⁺ (Kd = 1.3 nM, ex/em = 488 nm/515 nm)DepositorInsertGZnP3
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMega-MaPylRS
Plasmid#200225PurposeThe plasmid encodes an orthogonal aminoacyl-tRNA synthetase/tRNA-CUA pair for amber codon suppression with Nε-Boc-lysine.DepositorInsertsPyrrolysyl-tRNA synthetase
pyrrolysyl-tRNA
UseTagsnoneExpressionBacterialMutationnonePromoterproK-lacO and tacIAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide-zeo
Plasmid#160091PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and zeocin resistance markerDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pmScarlet-I_peroxisome_C1
Plasmid#85065PurposeIn vivo visualization of the peroxisome (can be used for colocalization studies)DepositorInsertPeroxisomal targeting sequence
UseTagsmScarlet-iExpressionMammalianMutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-GFP-progerin
Plasmid#17663DepositorInsertGFP-D50 lamin A (LMNA Human)
UseRetroviralTagsEGFPExpressionMammalianMutationDelta 50PromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AT110.i1
Plasmid#135481PurposeE. coli periplasmic expression of high-affinity conformational nanobody Nb.AT110.i1 recognizing angiotensin II type 1 receptorDepositorInsertNanobody AT110.i1
UseTags6xHis-tag and pelB leaderExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_mNG3A(1-10)
Plasmid#157992PurposeLentiviral vector of constitutive expression of mNeonGreen3A(1-10)DepositorInsertpSFFV_mNG3K(1-10)
UseLentiviralTagsExpressionMammalianMutationW148L, M150V, K153M, N194K, F204LPromoterSFFVAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
AbVec_IGHC
Plasmid#183701PurposeEncode human IgG1 heavy chain constant region. For cloning variable region and mAb expression.DepositorInsertIGHG1 (IGHG1 Human)
UseTagsExpressionMammalianMutationPromoterSP6Available sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
COVID-SARS2 NSP13
Plasmid#159614PurposeBacterial expression plasmid for COVID-SARS2 NSP13 helicaseDepositorInsertCovid-SARS2 Nsp13
UseTagsHis6-Zb-TEVExpressionBacterialMutationCodon-optimized for E. coli expressionPromoterT7 - LacOAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-22b DT 51E/148K
Plasmid#11081DepositorInsertDT
UseTagsHisExpressionBacterialMutation51E/148K, catalytically inactivePromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOTTC446 - pAAV EF1a hPREP(S554A)
Plasmid#59968PurposeAn AAV packaging vector that expresses catalytically-inactive hPREP under control of the EF1a promoter.DepositorInsertProlyl oligopeptidase (PREP Human)
UseAAVTagsExpressionMammalianMutationS554APromoterEF1aAvailable sinceOct. 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-GFP
Plasmid#58867PurposeAAV expression of GFP under the Syn promoterDepositorInsertGFP
UseAAVTagsExpressionMammalianMutationPromoterSynAvailable sinceAug. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
TFORF2534
Plasmid#142955PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX6 (SOX6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-p3E-HyPer7
Plasmid#194289PurposeMultisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQVDepositorInsertHyper7
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMF1762
Plasmid#125583PurposeExpresses mitochondrial roGFP2 (mt-roGFP2) in mammalian cellsDepositorInsertmt-roGFP2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
m6A-Tracer-NES
Plasmid#159607PurposeConstitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.DepositorInsertm6A-Tracer-NES
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Tight-Puro_Olig2
Plasmid#64842PurposeA lentiviral vector for the expression of mouse Olig2 under doxycyline controlDepositorInsertOlig2 (Olig2 Mouse)
UseLentiviralTagsExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-fba_RiboJ_GFP+_MGApt_Bba_B0015
Plasmid#107582PurposeB. megaterium DSM319 fba promoter, GFP+ with malachite green mRNA aptamer for Bacillus cell-free transcription-translation. Bacillus shuttle vector backbone, colE1/AmpR (E. coli), RebB/TetA (Bacillus)DepositorInsertGFP+
UseSynthetic Biology; E. coli and bacillus shuttle v…TagsB. megaterium DSM319 xylA leader sequence (MTSSKI…ExpressionBacterialMutationF64L/ S65T/ Q80R/ F99S/ M153T/ V163APromoterfba promoter Bacillus megaterium DSM319Available sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only