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Showing: 1141 - 1160 of 16047 results

  1. pGAPTrap-IRESMuro

    Plasmid
    #82336
    Purpose
    Base vector targeting genes to the GAPDH locus of human cells. Inserted genes are expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    IRESMuro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    82336-plasmid-map-sequence-id-155824

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  2. LCV2_BFP2

    Plasmid
    #155097
    Purpose
    Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression
    Depositor
    Jason Moffat
    Article
    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 7, 2020
    Availability
    Academic Institutions and Nonprofits only
    155097-plasmid-map-sequence-id-308002

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  3. pGAPTrap-mtagtdTom_IRESMuro

    Plasmid
    #82355
    Purpose
    Vector targeting the mTagtandem tomato gene to the GAPDH locus of human cells. It is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    mtagTandemTomato-IRESMuro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    82355-plasmid-map-sequence-id-156569

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  4. pGAPTrap-eGFP-IRESMuro

    Plasmid
    #82504
    Purpose
    Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    eGFP-IRESMuro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82504-plasmid-map-sequence-id-156573

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  6. pGAPTrap-LacZ-IRESMuro

    Plasmid
    #82507
    Purpose
    Vector targeting the LacZ gene to the GAPDH locus of human cells. LacZ is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    LacZ-IRESMuro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
    82507-plasmid-map-sequence-id-156577

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  7. Lenti-SAM-Puro

    Plasmid
    #198713
    Purpose
    For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.
    Depositor
    Tyler Jacks
    Article
    Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1
    Inserts
    U6-MS2-gRNAscaffold
    EF1a-MPH-Puro
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a and U6
    Available since
    Jan. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    198713-plasmid-map-sequence-id-402626

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  11. pGAPTrap-Clover-Muro

    Plasmid
    #82334
    Purpose
    Vector targeting the Clover gene to the GAPDH locus of human cells. Clover is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.
    Depositor
    Ed Stanley
    Article
    Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221
    Insert
    Clover-T2AMuro
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    82334-plasmid-map-sequence-id-156570

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  12. pLVX ALIX-GFP WT

    Plasmid
    #138582
    Purpose
    Expresses fluorescent ALIX in mammalian cells
    Depositor
    Wesley Sundquist
    Insert
    ALIX (PDCD6IP Human)
    Use
    Lentiviral
    Tags
    GFP and h30
    Expression
    Mammalian
    Mutation
    Promoter
    TREtight
    Available since
    Dec. 10, 2024
    Availability
    Academic Institutions and Nonprofits only
    138582-plasmid-map-sequence-id-275088

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  13. pLenti6.2_mCherry_ mito_roGFP2-Orp1

    Plasmid
    #155042
    Purpose
    Fluorescent redox reporter
    Depositor
    Vanessa LaPointe
    Insert
    Orp1 (HYR1 Budding Yeast)
    Use
    Lentiviral
    Tags
    mitochondrial targeting sequence and roGFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    155042-plasmid-map-sequence-id-311567

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  14. LT3GEPIR-CDK4shRNA1

    Plasmid
    #220574
    Purpose
    To inducibly knockdown CDK4 expression
    Depositor
    Sean Lee
    Insert
    CDK4 shRNA1 (CDK4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220574-plasmid-map-sequence-id-437920

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  15. LT3GEPIR-CDK4shRNA2

    Plasmid
    #220575
    Purpose
    To inducibly knockdown CDK4 expression
    Depositor
    Sean Lee
    Insert
    CDK4 shRNA2 (CDK4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    220575-plasmid-map-sequence-id-437919

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  16. pPB-US-ECasE

    Plasmid
    #83961
    Purpose
    Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.
    Depositor
    Eleanor Chen
    Insert
    ERT2-spCas9-ERT2
    Use
    Tags
    Expression
    Mammalian
    Mutation
    please see depositor comments below
    Promoter
    U6 (gRNA), EF1a (ERT2-Cas9-ERT2)
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83961-plasmid-map-sequence-id-214594

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  17. pPL7552_ColE1_PiggyBac_2xcHS4::mCherry-IRES-PuroR-P2A-mTagBFP2-NLS::2xcHS4

    Plasmid
    #217971
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.
    Depositor
    Robert Piper
    Insert
    2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    217971-plasmid-map-sequence-id-436106

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  18. lentiCRISPR.v2.TetON_Cas13d_U6_DR

    Plasmid
    #196727
    Purpose
    Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-miRFP670nano
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    TRE
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196727-plasmid-map-sequence-id-389689

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  19. Lazarus Gata 2 flag

    Plasmid
    #80137
    Purpose
    retroviral expression of Gata2-Flag
    Depositor
    Ellen Rothenberg
    Insert
    Gata2 (Gata2 Mouse)
    Use
    Retroviral
    Tags
    FLAG and IRES-EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    80137-plasmid-map-sequence-id-157353

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  20. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

    Plasmid
    #121174
    Purpose
    Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines
    Depositor
    Timo Otonkoski
    Insert
    MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    121174-plasmid-map-sequence-id-234993

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Showing: 1141 - 1160 of 16047 results