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Plasmid#217971PurposeProvides expression from a CMV promoter surrounded by insulators. Integrates via piggyBac transposase. Confers puromycin resistance. Expresses nuclear localized BFP. High copy plasmid.DepositorInsert2xcHS4::mCherry-IRES-PuroR-mTagBFP2-NLS::2xcHS4
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR.v2.TetON_Cas13d_U6_DR
Plasmid#196727PurposeSortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.DepositorInsertRfxCas13d-T2A-miRFP670nano
UseCRISPR and LentiviralTagsNucleoplasmin NLS and SV40 NLSExpressionMutationPromoterTREAvailable sinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
CROPseq-EFS-HypaSpCas9-P2A-puro
Plasmid#106965Purposesingle-vector CROPseq system with high fidelity SpCas9 (HypaSpCas9) for use in the CROPseq CRISPR knockout screenDepositorInsertHypaSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceApril 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSI9-U6-(sh)-UbiC-TagRFP-2A-Puro
Plasmid#28289Purpose3rd generation lentiviral plasmid; This plasmid is the backbone for the DECIPHER libraries (currently DISCONTINUED)DepositorTypeEmpty backboneUseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 20, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLVXPuro-mGFP-LIC1
Plasmid#66600PurposeExpresses Dynein-1 LIC1 as a mGFP fusionDepositorInsertLIC1 (DYNC1LI1 Human)
UseLentiviralTagsmGFPExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2MP.EF-QUE37C
Plasmid#129332PurposeATP measurement, Tol2 transposonDepositorInsertQUEEN-37C
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-US-ECasE
Plasmid#83961PurposePiggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.DepositorInsertERT2-spCas9-ERT2
UseTagsExpressionMammalianMutationplease see depositor comments belowPromoterU6 (gRNA), EF1a (ERT2-Cas9-ERT2)Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_ mito_roGFP2-Orp1
Plasmid#155042PurposeFluorescent redox reporterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseLentiviralTagsmitochondrial targeting sequence and roGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
1271 pBabe puroL Myr HA Akt2
Plasmid#9018DepositorInsertAkt2 (AKT2 Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLVXPuro-mGFP-LIC2
Plasmid#66601PurposeExpresses Dynein-1 LIC2 as a mGFP fusionDepositorInsertLIC2 (DYNC1LI2 Human)
UseLentiviralTagsmGFPExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDH-PGK-Nluc-P2A-copGFP-T2A-Puro
Plasmid#73040PurposeExpress Nluc and copGFP dual reporter in mammalian cellsDepositorInsertNluc-P2A-copGFP-T2A-Puro
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterPGK (phosphoglycerate kinase 1)Available sinceJan. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
LZRS-Rfa
Plasmid#31601DepositorTypeEmpty backboneUseRetroviralTagsExpressionMutationPromoterAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLEX307-TMPRSS4-puro
Plasmid#158472PurposeConstitutive lentiviral expression of human TMPRSS4.DepositorInsertTMPRSS4 (TMPRSS4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
EMSV-myogenin (puro)
Plasmid#14712DepositorInsertmyogenin (Myog Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
Lazarus Gata 2 flag
Plasmid#80137Purposeretroviral expression of Gata2-FlagDepositorInsertGata2 (Gata2 Mouse)
UseRetroviralTagsFLAG and IRES-EGFPExpressionMammalianMutationPromoterAvailable sinceAug. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
integrin beta6 pBabe puro
Plasmid#13596DepositorInsertintegrin beta 6 (ITGB6 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 8, 2007AvailabilityAcademic Institutions and Nonprofits only -
EMSV-MRF4 (puro)
Plasmid#14713DepositorInsertMRF4 (Myf6 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBCN22-R4R3
Plasmid#26302DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBCN21-R4R3
Plasmid#26301DepositorTypeEmpty backboneUseTagsExpressionWormMutationPromoterAvailable sinceDec. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIRES Centrin1 GFP Pur
Plasmid#64341PurposeBicistronic expression of Centrin-GFP fusion and Puro resistance geneDepositorInsertCentrin1 (CETN1 Human)
UseTagsGFP and IRES-PuroExpressionMammalianMutationPromoterCMVAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only