Skip to main content
Addgene
Showing: 11481 - 11500 of 14803 results

  3. pSB1A3 EcoRI Methylase-AmilCP

    Plasmid
    #85166
    Purpose
    For methylating EcoRI recognition sites in order to prevent cleavage by the EcoRI restriction enzyme. AmilCP is a blue reporter for Methylase expression.
    Depositor
    Robin Dowell
    Article
    Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71
    Insert
    EcoRI Methylase-AmilCP
    Use
    Tags
    AmilCP
    Expression
    Bacterial
    Mutation
    Promoter
    J23100 promoter
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pMpGWB415

    Plasmid
    #68680
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Takayuki Kohchi
    Article
    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do
    Type
    Empty backbone
    Use
    Tags
    Nuclear localization signal-tagged Citrine (Citri…
    Expression
    Plant
    Mutation
    Promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    68680-plasmid-map-sequence-id-121801

    Enlarge

  6. pFGL1170R

    Plasmid
    #116896
    Purpose
    hH1:mCherry (nuclear marker)
    Depositor
    Naweed Naqvi
    Article
    Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581
    Inserts
    hH1
    mCherry
    hH1 downstream region
    Use
    Fungal expression (in magnaporthe oryzae)
    Tags
    Expression
    Mutation
    no start codon, no stop codon and without start c…
    Promoter
    promoter less
    Available since
    Aug. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    116896-plasmid-map-sequence-id-232119

    Enlarge

  7. GFP-CmTMC1truncated

    Plasmid
    #154908
    Purpose
    For the expression and purification of the truncated TMC1 protein from Chelonia mydas. The protease cleavage site was replaced with the HRV 3C site.
    Depositor
    Motoyuki Hattori
    Article
    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d
    Insert
    GFP-CmTMC1truncated
    Use
    Tags
    8histidine-EGFP
    Expression
    Insect
    Mutation
    Truncations of the residues 1-243 and 843-969
    Promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    154908-plasmid-map-sequence-id-310626

    Enlarge

  8. GFP-MuTMC2truncated

    Plasmid
    #154909
    Purpose
    For the expression and purification of the truncated TMC2 protein from Melopsittacus undulates. The protease cleavage site was replaced with the HRV 3C site.
    Depositor
    Motoyuki Hattori
    Article
    Jia et al Neuron. 2020 Jan 22;105(2):310-321.e3. d
    Insert
    GFP-MuTMC2truncated
    Use
    Tags
    8histidine-EGFP
    Expression
    Insect
    Mutation
    Truncations of the residues 1-179 and 691-716
    Promoter
    Available since
    July 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    154909-plasmid-map-sequence-id-306870

    Enlarge

  9. pUE1-TSP-ORF68 K395A/K396A

    Plasmid
    #162656
    Purpose
    Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site
    Depositor
    Britt Glaunsinger
    Article
    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1
    Insert
    ORF68
    Use
    Tags
    N-terminal 2xStrep-Tag II and HRV 3C site
    Expression
    Mammalian
    Mutation
    Amino acids K395 and K396 mutated to alanine
    Promoter
    CMV
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    162656-plasmid-map-sequence-id-320674

    Enlarge

  12. DPAGT1 (DPAGT1A-c005)

    Plasmid
    #98228
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DPAGT1A (DPAGT1 Human)
    Use
    Baculovirus
    Tags
    His-6-TEV
    Expression
    Mutation
    pfam00953:Glycos_transf_4 98-275
    Promoter
    polyhedrin
    Available since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98228-plasmid-map-sequence-id-193399

    Enlarge

  13. pMV-URR2

    Plasmid
    #65332
    Purpose
    standard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUs
    Depositor
    Junbiao Dai
    Article
    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4
    Insert
    URR2
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    65332-plasmid-map-sequence-id-137535

    Enlarge

  14. pMV-URR1

    Plasmid
    #65331
    Purpose
    standard plasmids for exogenus pathway assembly haboring recombination sequence 1(Universal Recombination Region 1) which could be released and ligated to series of TUs
    Depositor
    Junbiao Dai
    Article
    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4
    Insert
    URR1
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    65331-plasmid-map-sequence-id-110564

    Enlarge

  15. p5B3-T

    Plasmid
    #92021
    Purpose
    Expresses TALE targeted to lysA promoter with one TEV cut site in its N-terminal domain, anhydrotetracycline inducible TEV protease
    Depositor
    Brian Pfleger
    Article
    Copeland et al Nat Chem Biol. 2016 Apr;12(4):254-60. do
    Inserts
    TALE targeting lysA with N-terminal TEV protease cleavage site
    Tobacco Etch Virus Protease
    Use
    TALEN
    Tags
    5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…
    Expression
    Mutation
    S219V
    Promoter
    Available since
    June 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92021-plasmid-map-sequence-id-181444

    Enlarge

  19. p667-UBC-DYRK3-V5-miniTurbo_IDG-K

    Plasmid
    #158190
    Purpose
    Gateway destination clone of DYRK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    DYRK3-V5-miniTurbo (DYRK3 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-miniTurbo
    Expression
    Mammalian
    Mutation
    R293H- please see depositor comments
    Promoter
    Ubiquitin
    Available since
    April 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    158190-plasmid-map-sequence-id-328919

    Enlarge

  20. pGBW-m4046952

    Plasmid
    #145718
    Purpose
    Bacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;FLAG_2
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145718-plasmid-map-sequence-id-307567

    Enlarge

Showing: 11481 - 11500 of 14803 results