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Plasmid#130677PurposeExpresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-46.47A-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationM1961_E1992delPromoterCMVAvailable sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG
Plasmid#130691PurposeExpresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
UseTags3xFLAG and 3xHAExpressionMammalianMutationE653_G1960delPromoterCMVAvailable sinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG
Plasmid#130689PurposeExpresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon37-46.47A-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutation1M_T1522delinsM, M1961_E1992delPromoterCMVAvailable sinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.47M-48-3xFLAG
Plasmid#130686PurposeExpresses human SMCHD1 Exon1-9.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_G1960delPromoterCMVAvailable sinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS attB V5 dDispatched WT HA
Plasmid#126411PurposeExpression of wild type dDispatched HA in Drosophila.DepositorInsertd-Dispatched1 (disp Fly)
UseTagsHAExpressionInsectMutationPromoterAvailable sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG
Plasmid#130690PurposeExpresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutation1M_T1522delinsM, G1720_Y1846delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.41-48-3xFLAG
Plasmid#130685PurposeExpresses human SMCHD1 Exon1-9.41-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.41-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_Q1684delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-14.47M-48-3xFLAG
Plasmid#130682PurposeExpresses human SMCHD1 Exon1-14.47M-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-14.47M-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationE653_G1960delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-SMCHD1-Exon1-9.47A-48-3xFLAG
Plasmid#130687PurposeExpresses human SMCHD1 Exon1-9.47A-48 with a C-terminal 3xFLAG tagDepositorInsertSMCHD1 Exon1-9.47A-48 (SMCHD1 Human)
UseTags3xFLAGExpressionMammalianMutationI378_E1992delPromoterCMVAvailable sinceSept. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-RTRG
Plasmid#69845PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert1 RTRG repeat
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)2
Plasmid#69846PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert2 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)3
Plasmid#69847PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert3 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-(RTRG)4
Plasmid#69848PurposeThe RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeatsDepositorInsert4 RTRG repeats
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTXB1-EXO1b
Plasmid#68267PurposeBacterial expression of human EXO1DepositorInsertEXO1 (EXO1 Human)
UseTagsMxe intein/chitin binding domainExpressionBacterialMutationb form, codons 1-846. Please see depositor commen…PromoterT7Available sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9-sgNT
Plasmid#140238PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a control non-targeting sgRNADepositorInsertnon-targeting sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-eCas9-sgMIB1
Plasmid#140239PurposeLentiviral vector expressing high-specificity eSpCas9(1.1) and a gRNA targeting human MIB1DepositorInsertsgRNA targeting human MIB1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
NLS pEGFP-N3 (1299)
Plasmid#62043Purposemammalian expression of NLS-EGFPDepositorInsertSV40 NLS
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pALS099 (pGEX-2TP-cTevHis-DEST)
Plasmid#139787PurposeGateway destination vector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneUseTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialMutationPromoterAvailable sinceApril 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC72
Plasmid#118866Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEVDepositorInsertNcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, mGFP, cleavable…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only