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pcDNA-GFP-EBA175-Flag

Plasmid

#154970

Purpose

Expresses N-terminally GFP-tagged EBA175 (residues 1284-1462) from pcDNA3.1. Please note: Plasmid contains a Stop codon prior to the C-terminal Flag tag.

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

GFP and signal peptide (residues 1 to 32) from TG…

Expression

Mammalian

Mutation

insert codes for EBA175 residues 1284 to 1462

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pGL3-Promoter-4xBS_BHLHE40

Plasmid

#153064

Purpose

Four BHLHE40 consensus binding sites cloned upstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

Insert

4X consensus BHLHE40 binding sites

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
pDS2142

Plasmid

#184273

Purpose

Tet-off genome insertion system

Depositor

Dominique Sanglard

Article

Lemberg et al PLoS Pathog. 2022 Apr 11;18(4):e1010012.

Insert

CatTA

Use

Tags

Expression

Yeast

Mutation

Promoter

TDH3

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pLPCX2-FLAG-Smad4

Plasmid

#89834

Purpose

Express Smad4 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

Insert

SMAD4 (SMAD4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88-FW/AA

Plasmid

#12289

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

F285A/W286A

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-HA-MyD88-I179N

Plasmid

#12295

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

I179N

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-HA-MyD88-P200H

Plasmid

#12288

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

P200H

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pGAL4-DBD-RUNX2wt-IDR

Plasmid

#145252

Purpose

Expresses fusion of GAL4 DNA-binding domain and RUNX2Awt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2Awt-IDR

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-RUNX2+10A-IDR

Plasmid

#145251

Purpose

Expresses fusion of GAL4 DNA-binding domain and RUNX2A+10A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

RUNX2A+10A-IDR

Use

Tags

Expression

Mammalian

Mutation

A-repeat expanded

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAGM_Puro_shRNA n.2_mTet3

Plasmid

#79625

Purpose

Expression of shRNA for mouse Tet3

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet3 (Tet3 Mouse)

Use

Lentiviral

Tags

none

Expression

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pAGM_Puro_shRNA n.1_mTet3

Plasmid

#79624

Purpose

Expression of shRNA for mouse Tet3

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet3 (Tet3 Mouse)

Use

Lentiviral

Tags

none

Expression

Mutation

Promoter

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pRset-rogRFP2

Plasmid

#207875

Purpose

Bacterial expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GW1-rogRFP2

Plasmid

#207876

Purpose

Mammalian expression of the red fluorescent rogRFP2 redox sensor

Depositor

Mathew Tantama

Article

Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

Insert

rogRFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-DIO-basic

Plasmid

#196882

Purpose

Used for preparation of double-floxed inverse ORF vectors

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Type

Empty backbone

Use

Cre/Lox; Dre/rox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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TERTpWT_Gluc_CCR5

Plasmid

#192270

Purpose

Donor template for insertion of hTERT promoter driven Gaussia luciferase reporter

Depositor

Shantanu Chowdhury

Article

Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

Insert

Promoter Reporter Insert within donor template

Use

CRISPR

Tags

Expression

Mutation

Promoter

TERT promoter driving expression of gaussia

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pFRTpsiCHECK_SEAP

Plasmid

#154461

Purpose

Expression of SEAP and firefly luciferase

Depositor

Markus Landthaler

Article

Zinnall et al Nat Commun. 2022 May 18;13(1):2727. doi:

Insert

SEAP

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-AMA1-Flag(K546M/I547P/I548Y/I549Y)

Plasmid

#155308

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged AMA1 K546M/I547P/I548Y/I549Y variant

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfAMA1

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

K546M/I547P/I548Y/I549Y

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EBA175-Flag(Y1426S)

Plasmid

#155035

Purpose

Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 Y1426S variant (residues 1284-1462) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

Y1426S; insert codes for EBA175 residues 1284 to …

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-EBA175-Flag(P1424G)

Plasmid

#155128

Purpose

Expresses N-terminally GFP-tagged EBA175 P1424G variant (residues 1284-1462) from pcDNA3.1

Depositor

Sinisa Urban

Article

Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

Insert

PfEBA175

Use

Tags

Flag, GFP, and signal peptide (residues 1 to 32) …

Expression

Mammalian

Mutation

P1424G; insert codes for EBA175 residues 1284 to …

Promoter

CMV

Available since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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