Showing: 11321 - 11340 of 20580 results
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Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta
Plasmid#169240PurposeCas9 and beta expression plasmid for performing gene knockouts via oligo recombineeringDepositorInsertscas9
beta
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pKL2013
Plasmid#172182PurposeA CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.DepositorInsertmCherry
UseCRISPRTagsExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailabilityAcademic Institutions and Nonprofits only -
pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro
Plasmid#174118PurposeExpression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)DepositorInsertsgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro
UseLentiviralTagsExpressionMutationPromotermU6 and UbCAvailabilityAcademic Institutions and Nonprofits only -
pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro
Plasmid#174119PurposeExpression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)DepositorInsertsgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro
UseLentiviralTagsExpressionMutationPromotermU6 and UbCAvailabilityAcademic Institutions and Nonprofits only -
pAT15484_HF-ABE8e
Plasmid#174122PurposeExpresses ABE8e fused with SpCas9-HF1DepositorInsertHF-ABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailabilityAcademic Institutions and Nonprofits only -
pAT15485_Hypa-ABE8e
Plasmid#174123PurposeExpresses ABE8e fused with Hypa-SpCas9DepositorInsertHypa-ABE8e
UseCRISPRTagsNucleoplasmin NLS and SV40 NLSExpressionMammalianMutationPromoterEF1-aAvailabilityAcademic Institutions and Nonprofits only -
pJEC626
Plasmid#174367PurposeActivation domain for modular CRISPRa. AD-SYNZIP. Expresses alphaNTD from the E coli RNAP with a SYNZIP17 domain fused to the N terminus.DepositorInsertAlpha N-terminal domain from the E. coli RNA polymerase fused to SYNZIP17
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23106AvailabilityAcademic Institutions and Nonprofits only -
pBM23-pyrF-NT
Plasmid#174384PurposepBM23 contained pyrF and mini-CRISPR.DepositorInsertpyrF-NT
UseTemplate for cloning fragments for chromosomal in…TagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pBM23-pyrF-CS
Plasmid#174396PurposepBM23 contained pyrF and mini-CRISPR.DepositorInsertpyrF-CS
UseTemplate for cloning fragments for chromosomal in…TagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAB1619 hU6-BpiI-Cas13bt1-DR
Plasmid#176314PurposehU6-BpiI-Cas13bt1-DR (crRNA expression)DepositorInsertCas13bt1 crRNA direct repeat
UseCRISPRTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-HNHx-ABEmax7.10HD-BlastR
Plasmid#176477PurposeA-to-G base editor with alternative targeting scopeDepositorInsertHNHx-ABEmax7.10HD
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
p2T-CMV-HNHx-ABE8eHD-BlastR
Plasmid#176478PurposeA-to-G base editor with alternative targeting scopeDepositorInsertHNHx-ABE8eHD
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pX459-SpCas9-NG
Plasmid#171370PurposeSpCas9-NG-mediated genome editing.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6 for sgRNA; CBh for Cas9-2A-puroRAvailabilityAcademic Institutions and Nonprofits only -
psgRNAv1-empty
Plasmid#171611PurposesgRNA_v1 plasmid for AsCas12f1 in bacteriaDepositorInsertAsCas12f1_sgRNA_1
UseTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pJCC_058 FnCas12a D917A
Plasmid#171667PurposeFor bacterial expression of FnCas12a D917A (RuvC-inactivating mutation) with a C-terminal sorting motif and His tagDepositorInsertFnCas12a D917A
UseTags6X His and sortase A sorting motifExpressionBacterialMutationchanged aspartate 917 to alaninePromoterAvailabilityAcademic Institutions and Nonprofits only -
pJCC_084 Cas12i1 D647A
Plasmid#171671PurposeFor bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertCas12i1 D647A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged aspartate 647 to alaninePromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV_ABE‐NG‐loop
Plasmid#187057PurposeABEmax-SpCas9-NG-loopDepositorInsertABE‐NG‐loop
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pnxABE-flgn
Plasmid#187932PurposeA-to-G base editorDepositorInsertecTadA(8e)-nxCas9 (D10A)
UseCRISPRTagsExpressionBacterialMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCMV-SpRY-ABE8e
Plasmid#185671PurposeExpresses SpRY-ABE8e in mammalian cellsDepositorInsertecTadA(8e)-nSpRY
UseCRISPRTagsExpressionMammalianMutationSpRY D10APromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 11321 - 11340 of 20580 results