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Plasmid#183040PurposeExpresses the FLAG-tagged GeNL (Green Nanolantern) in mammalian cells.DepositorInsertGeNL Nanolantern
UseLentiviralTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Calnexin-HT8-mGold
Plasmid#183989PurposeExpresses fluorescent HaloTag8 fusion protein in mammalian cells with localization in the ER.DepositorInsertCalnexin (CANX Human)
UseTagsHaloTag8 (C61S, C262S), mGoldExpressionMammalianMutationPromoterAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pYPQ133-tRNA2.0
Plasmid#158395PurposeGolden Gate entry vector to express the 3rd gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV4a-SIRT6-H133Y-FLAG
Plasmid#102324PurposeHuman SIRT6 H133Y with FLAG tag for mammalian expressionDepositorInsertSIRT6 (SIRT6 Human)
UseTagsFLAGExpressionMammalianMutationchanged histidine 133 to tyrosinePromoterCMVAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SIM)1
Plasmid#127094PurposeBacterial expression plasmid for mCherry-(SIM)1 client to assess SUMO-SIM scaffolds.DepositorInsertmCherry - SUMO interacting motif (SIM) from PIASx (PIAS2 Human)
UseTagsmCherryExpressionBacterialMutationPromoterunknownAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcI-ts,ind+-(mtSSB delta20)
Plasmid#186280PurposeExpresses Human Mitochondrial SSB in E. coliDepositorInsertMitochondrial Single Stranded DNA Binding Protein (SSBP1 Human)
UseTagsExpressionBacterialMutationFirst 20 amino acids corresponding to probable mi…PromoterlambdaAvailable sinceJuly 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
ACTIN-SOX2-PGK Cre
Plasmid#59019PurposeBicistronic Lentivirus expressing sox2 and CreDepositorInsertSox2 (Sox2 Mouse)
UseLentiviralTagsExpressionMutationPromoterB-actinAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA2.0
Plasmid#158396PurposeGolden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL0374 (His-MBP-Cas8_Cas7_Cas6)
Plasmid#130639PurposeExpresses V. cholerae CAST Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on Cas8 for purification.DepositorInsertVchCas8, VchCas7, VchCas6
UseCRISPR; TransposonTagsHis10-MBP-TEVsite on Cas8ExpressionBacterialMutationPromoterT7Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-FUCCI
Plasmid#133363PurposePiggybac vector for shRNA-mediated knockdown, co-expressed with FUCCI cell cycle reporterDepositorInsertClover-Geminin-IRES-mKO2-Cdt1
UseTagsHA-tagged Clover-GemininExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO)3
Plasmid#127150PurposeMammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsert3 copies of human SUMO1 separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP 3xMyc-STX17ΔNTD
Plasmid#89940PurposeExpresses syntaxin17 lacking N-terminal domain tagged with Myc in mammalian cellsDepositorInserthuman syntaxin17 lacking N-terminal domain (STX17 Human)
UseRetroviralTagsMycExpressionMutationdeleted amino acids 1-161PromoterAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP12-CB2x2-mTagBFP2-HAx2
Plasmid#207146PurposeTandem calcium-buffer with rapalog dimerizerDepositorInsertCalbindin-2 (CALB2 Human)
UseTagsFKBP12, HA, and mTagBFP2ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-MKRN1
Plasmid#78745PurposeTo overexpress MKRN1 in Mammalian CellsDepositorInsertMKRN1 (MKRN1 Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-CAAX
Plasmid#51567PurposeConstitutively express PM (Plasma membrane) localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsCAAX and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
YFP Gamma 2
Plasmid#36102DepositorInsertG protein subunit Gamma 2 (GNG2 Bovine)
UseTagsYFPExpressionMammalianMutationPromoterCMVAvailable sinceMay 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET12a-AL-09 FL
Plasmid#47081DepositorInsertAL-09 (IGKV3-20 Human)
UseTagsompTExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135-tRNA2.0
Plasmid#158397PurposeGolden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterWithout promoterAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only