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Plasmid#166033PurposeExpression of 6X MS2 stem loops fused SpCas9 mRNA for packaging of SpCas9 mRNA within lentiviral particles.DepositorInsertSpCas9
UseCRISPR and LentiviralTagsExpressionMutationPromoterCMVAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-hChR2(H134R)-mCherry
Plasmid#26976PurposeAAV expression of humanized ChR2 with H134R mutation fused to mCherry, for optogenetic excitationDepositorHas ServiceAAV5, AAV8, and AAV9InserthChR2(H134R)
UseAAVTagsmCherryExpressionMammalianMutationH134RPromoterhSynAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCAG-EGFP(HiBiT)
Plasmid#162589PurposeN-terminally HiBiT-tagged EGFP under CAG promoterDepositorInsertEGFP
UseTagsHiBiTExpressionMammalianMutationPromoterCAG promoterAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-hGSDME
Plasmid#154876PurposeExpresses hGSDME in mammalian cellsDepositorInsertGSDME (GSDME Human)
UseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-GCaMP6s
Plasmid#135418PurposeCan be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.DepositorInsertGCaMP6s
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOpen-SP6rpol
Plasmid#165516PurposeDNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.DepositorInsertSP6 RNA Polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 29, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
UseTagsAmilCP and RTXExpressionBacterialMutationPromoterJ23100 promoterAvailable sinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
LM627
Plasmid#191551PurposeLM627_MSG-AGGA-promoter-ccdb-TTCC-SNAC-hisDepositorInsertccdb
UseTagsSNAC-tag, HIS-tagExpressionBacterialMutationPromoterAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DsRed
Plasmid#11151PurposeMammalian expression vector for expression of DsRedDepositorHas ServiceCloning Grade DNAInsertDsRed2 from Discosoma sp
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pVDL9.3
Plasmid#168299PurposeSecretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyADepositorInsertSegment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
UseTagsExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
TagBFP2-PIP4K2C
Plasmid#202730Purposeexpresses fluorescent protein-tagged enzymeDepositorInsertmTagBFP2:PIP4K2C (PIP4K2C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1-GfaABC1D-GAT3-HA-mCherry
Plasmid#117722PurposeExpresses a GABA transporter GAT-3 (Slc6a11)DepositorInsertSlc6a11 (Slc6a11 Mouse)
UseAAVTagsHA and mCherryExpressionMammalianMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (AAV1)
Viral Prep#104496-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE (#104496). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP7f-WPRE plasmid DNA. CAG-driven, Cre-dependent jGCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterCAGTagsNoneAvailable sinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPGK-Cre-bpA
Plasmid#11543DepositorInsertPhosphoglycerate kinase 1 promoter
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2006AvailabilityAcademic Institutions and Nonprofits only -
pST39-pNL29
Plasmid#91696PurposeExpresses Mega GVs in E.ColiDepositorInsertMega GV gene cluster (from pNL29) in pST39 backbone
UseTagsNo tagsExpressionBacterialMutationPromoterT7Available sinceOct. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
EcoFlex MoClo Kit
Plasmid Kit#1000000080PurposeGolden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit designDepositorAvailable sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-IL2 C125S
Plasmid#104348PurposeExpresses human IL-2 (C125S)DepositorInsertHuman IL-2 (C125S) (IL2 Human)
UseTagsMRGSHHHHHGS-tagExpressionBacterialMutationC125S mutation (TGT -> TCC)PromoterT5Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-UbC-FKRP-EF1a-BSD
Plasmid#205150PurposeExpress UbC-driven FKRP and EF-1a driven BSDDepositorInsertFukutin-related protein (FKRP Human)
UseLentiviralTagsExpressionMammalianMutationPromoterUbCAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (AAV Retrograde)
Viral Prep#51085-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-hSyn1-GCaMP6f-P2A-nls-dTomato (#51085). In addition to the viral particles, you will also receive purified AAV-hSyn1-GCaMP6f-P2A-nls-dTomato plasmid DNA. GCaMP6f calcium sensor and bicistronic, physically separate nuclear localized dTomato expression under a human synapsin1 promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsnuclear dTomato (physically separate, not a fusion protein)Available sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
LeGO-T2
Plasmid#27342DepositorInsertSFFV promoter, tdTomato
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2012AvailabilityAcademic Institutions and Nonprofits only