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Plasmid#115519PurposeTo construct multiple sgRNA expression vector for CRISPRDepositorInsertccdB-sgRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)
Plasmid#160571PurposeVersion of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.DepositorInsertsgRNAscaffold 2.1 scRNA
UseCRISPRTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Sth3)_ctrA
Plasmid#133339Purposefor constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgRNA(MS2)-puro-barcode
Plasmid#89493PurposeCloning backbone for sgRNA, also has a barcode that allows detection from scRNA-seqDepositorInsertsgRNA (with MS2 loop)
UseLentiviralTagsExpressionMutationPromoterU6Available sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-dSaCas9-pU6-sgRNA
Plasmid#159174PurposeVector B encodes pAAV-pMecp2-dSaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of dSaCas9-sgRNA in neuronsDepositorInsertdSaCas9
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterpMecp2Available sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)
Plasmid#71829PurposeExpression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a controlDepositorInsertBACH2-sgRNA8 (BACH2 Synthetic, Human, S. pyogenes)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9; E756A inactiva…PromoterU6Available sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 3
Plasmid#109003PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #3
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172670PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertSDHB pegRNA and SDHB_nick-sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
CMV:AsCpf1-2A-GFP-U6-sgRNA-cloning vector
Plasmid#194715PurposeBicistronic vector expressing CMV promoter driven AsCpf1, and U6 promoter driven guide RNA that can be cloned with BbsI sitesDepositorArticleInsertAsCpf1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_WT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro_SV40polyA
Plasmid#124546PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertWT-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_WT-hutRNApro
UseTagsExpressionMammalianMutationWT, WTPromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pU6-SasgRNA scaffold-CMV-nSaCas9
Plasmid#171695PurposeExpresses nSaCas9 in mammalian cellsDepositorInsertsgRNA scaffold-nSaCas9-NLS
UseCRISPRTagsExpressionMammalianMutationD10APromoterU6 and CMVAvailable sinceAug. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG1-puro Non-targeting sgRNA 1
Plasmid#109002PurposeCRISPRi knockdown of targeted gene (to be used with Addgene #102244 or other dCas9-KRAB constructs)DepositorInsertNon-targeting sgRNA #1
UseLentiviralTagsExpressionMammalianMutationPromotermU6 promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKSB-631 U6-sgRNA3/4 AACA GCTT
Plasmid#173675PurposeTo clone and express a gRNA in AnophelesDepositorTypeEmpty backboneUseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pShCAST(Cas12k-TniQ)-sgRNA_entry (CJT11)
Plasmid#181787PurposeExpresses 3-component ShCAST containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShTnsB, ShTnsC, ShCas12k-ShTniQ
UseTagsExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
8xCTS1_deltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro_SV40polyA
Plasmid#124542PurposeCloning vector for tRNA-released sgRNA. BsmBI flanked placeholder for spacer cloning.DepositorInsertdeltaC55G-hutRNApro_BsmBIplch-sgRNA_14nt Buffer_deltaC55G-hutRNApro
UseTagsExpressionMammalianMutationd11-41 C55G, d11-41 C55GPromoterMinimal ADEAvailable sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-slr0230-PL22-sgRNANT1-KmR
Plasmid#73224PurposeContains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coliDepositorInsertPL22-sgRNA NT1
UseTagsExpressionBacterialMutationPromoterPL22Available sinceApril 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA
Plasmid#107050PurposeAll-in-one vectors expressing both SaCas9 and YFP sgRNADepositorInsertSaCas9
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA
Plasmid#159283PurposeA multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).DepositorInsertsHuman INS sgRNA
Human INS guide RNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA
Plasmid#133342Purposefor constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentusDepositorInsertsgRNA_ctrA
UseCRISPRTagsExpressionMutationPromoterconstitutiveAvailable sinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172668PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertALDOB pegRNA and ALDOB_nick-sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only