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Plasmid#193580PurposeExpresses human Clathrin Light Chain B tagged with SNAP-tag and mEGFP in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsSNAP-tag and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-ShadowY-CLC
Plasmid#193552PurposeExpresses mouse Clathrin Light Chain A tagged with mEGFP and ShadowY in mammalian cells.DepositorInsertClathrin light chain A (Clta Mouse)
UseTagsShadowY and mEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
EGFP-CLCdeltaN
Plasmid#193562PurposeExpresses human Clathrin Light Chain B truncation mutant (deleted 1-89 aa) tagged with mEGFP in mammalian cells.DepositorInsertClathrin light chain B (CLTB Human)
UseTagsmEGFPExpressionMammalianMutationDeleted amino acids 1-89PromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV.GFAP.SV40.Thbs1-HA.SV40(polyA)
Plasmid#195552PurposeExpresses Thbs1 under control of short GFAP promoterDepositorInsertThrombospondin-1 (Thbs1 Mouse)
UseAAVTagsHA-tagExpressionMammalianMutationPromotershort GFAPAvailable sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
YW347_Ptbb2UTR-loxP-RFP-miniMOS
Plasmid#196064Purposea plasmid modified from miniMOS vector pCFJ910. the modification including insertions of myo2p::TagRFP downstream to NeoR CDS and 2 loxP sites flanking the NeoR and tagRFP cassettes.DepositorInsertsmyo2p driven tagRFP
NeoR
Minimal Mos1
UseTagsExpressionWormMutationPromotermyo-2Available sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E,K450R)
Plasmid#187881PurposeGal4/UAS expression of dSarm(isoE,K450R)DepositorInsertdSarm isoform E (Sarm )
UseTagsExpressionMutationK450RPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
deltaVP1d63C + VP2C-GFPdeg
Plasmid#182481PurposeYIp for expressing Murine polyomavirus deltaVP1 with the 63 C-ter residues truncated (GAL1 promoter) and destabilised GFP tagged with VP2C (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-GFPdeg
deltaVP1d63C
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES
Plasmid#182432PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-FPPS-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (M)
Plasmid#182434PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(M)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Linked VLP NES (G)
Plasmid#182476PurposeYIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).DepositorInsertsVP2C-FPPS(G)-NES
deltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV:eGFP-p2A-HA-GPR151(p.Arg95Ter)
Plasmid#190134Purposeexpresses eGFP-p2A-HA-GPR151 (pArg95Ter variant) in mammalian cellsDepositorInserteGFP-p2A-HA-GPR151(p.Arg95Ter)
UseLentiviralTagsHA, eGFP, and p2AExpressionMammalianMutationp.Arg95Ter mutationPromoterCMVAvailable sinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
W118-1_eGFP
Plasmid#180379Purposelentiviral transduction of eGFP geneDepositorInserteGFP (eGFP )
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
ABHD2-3'UTR-WT
Plasmid#190094Purposeexamine the activity of ABHD2 3'UTRDepositorInsertABHD2 3' UTR Wild Type (ABHD2 Human)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRps9-WT-IRES-GFP-HygR
Plasmid#190271PurposeExpress RPS9 WT mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.DepositorInsertribosomal protein RPS9
UseTagsIRES-eGFPExpressionMammalianMutationPromoterCAGGS-CMVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRps9-D95N-IRES-GFP-HygR
Plasmid#190272PurposeExpress RPS9 D95N-mutant mouse gene transcriptionally fused with eGFP. eGFP is translated from IRES.DepositorInsertribosomal protein RPS9
UseTagsIRES-eGFPExpressionMammalianMutationD95NPromoterCAGGS-CMVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLL5.0 CortW22A mCherry
Plasmid#187268PurposeLenticiral expression of mCherry-Cortactin W22ADepositorInsertCortactin (CTTN Human)
UseLentiviralTagsmcherryExpressionMutationW22APromoter5' LTRAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
2xNLS-NLP-cMyc AspCas12a
Plasmid#182120PurposepET21a protein expression vector for 2xNLS-NLP-cMyc AspCas12a in bacteriaDepositorInsert2xNLS-NLP-cMyc AspCas12a
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDARMO_CMVT_3xFLAG-HUWE1_YH/ GG_delta3291-3298
Plasmid#187127Purposemammalian cell expression of FLAG-HUWE1 Y355G/H356G, delta3291-3298DepositorInsertHUWE1 (HUWE1 Human)
UseTags3xFLAGExpressionMammalianMutationY355G/H356G, delta3291-3298PromoterAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229L-pET151
Plasmid#177879PurposeExpresses mouse Talin 1 F2F3-P229L domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Leucine (P229L)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229S-pET151
Plasmid#177878PurposeExpresses mouse Talin 1 F2F3-P229S domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Serine (P229S)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only