Addgene

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Showing: 11041 - 11060 of 24780 results

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pUHD.NFATc1nuc

Plasmid

#40616

Depositor

Jerry Crabtree

Article

Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

Insert

NFATc1nuc (NFATC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

S172A S175A S176A S178A S179A S181A S184A S187A S…

Promoter

CMV2

Available since

Oct. 22, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 human cofilin S3E

Plasmid

#50855

Purpose

expresses psuedo-phosporylated, non-activatable cofilin in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Use

Tags

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available since

Feb. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pFAB3737

Plasmid

#47838

Purpose

BIOFAB RFP reporter plasmid for measuring promoter 7 + BCD1 efficiency.

Depositor

Drew Endy

Article

Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

Insert

promoter 7 and BCD1

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

see insert

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV5-ratERK2-Q103A

Plasmid

#40816

Depositor

Natalie Ahn

Article

Emrick et al Proc Natl Acad Sci U S A. 2006 Nov 28;10

Insert

MAPK1 (Mapk1 Rat)

Use

Tags

His6

Expression

Mammalian

Mutation

Q103A

Promoter

CMV

Available since

Oct. 18, 2012

Availability

Academic Institutions and Nonprofits only
HOXA4 (murine) HA-tag pET

Plasmid

#8489

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXA4 (Hoxa4 Mouse)

Use

Tags

HA and T7

Expression

Bacterial

Mutation

Thr-2 changed to Ala-2 to form better ribosomal b…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pDM304_DPAKa(GBD)-YFP

Plasmid

#128699

Purpose

expression of YFP-tagged probe for active Rac1 in Dictyostelium discoideum cells

Depositor

Igor Weber

Article

Marinovic et al Histochem Cell Biol. 2016 Sep;146(3):267

Insert

GBD domain (aa 731-890) of DPAKa

Use

Dictyostelium discoideum expression vector

Tags

YFP

Expression

Mutation

none

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSEMS-SDHB-Halo7Tag

Plasmid

#108973

Purpose

Selflabeling tag at succinate dehydrogenase complex subunit B (SDHB)

Depositor

Karin Busch

Article

Wilkens et al J Cell Sci. 2013 Jan 1;126(Pt 1):103-16.

Insert

succinate dehydrogenase complex iron sulfur subunit B (SDHB Human)

Use

Tags

Halo7-tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 13, 2018

Availability

Academic Institutions and Nonprofits only

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NUDT16L1

Plasmid

#155795

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

NUDT16L1 (NUDT16L1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-RaichuEV-Rac1(T17N)

Plasmid

#164902

Purpose

Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

RaichuEV-Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

T17N mutation in the Rac1 domain which make the s…

Promoter

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pXPR_003 sgMYC guide 2

Plasmid

#193597

Purpose

cMYC knockout

Depositor

William Hahn

Article

Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

Insert

sgMYC guide 2 (MYC Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc-ARF

Plasmid

#19930

Depositor

Yue Xiong

Article

Zhang et al Cell. 1998 Mar 20. 92(6):725-34.

Insert

cyclin-dependent kinase inhibitor 2A p19ARF (CDKN2A Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pU6-sgGAL4-1

Plasmid

#46915

Purpose

Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GAL4 UAS promoter

Depositor

Stanley Qi, Jonathan Weissman

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Inserts

sgGAL4-1
Puromycin resistance and mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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Sema6c-Fc-His

Plasmid

#72167

Purpose

Expresses the extracellular region of the Sema6C protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema6c (Sema6c Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_C2517

Plasmid

#91086

Purpose

Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)

Use

CRISPR

Tags

Expression

Mutation

Promoter

TaU3

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pmTurquoise-parkin

Plasmid

#110945

Purpose

Expresses the ubiquitin ligase parkin fused to mTurquoise

Depositor

Pablo Rivera-Fuentes

Article

Nguyen et al Org Biomol Chem. 2021 Mar 28;19(12):2681

Insert

E3 ubiquitin ligase parkin (PRKN Human)

Use

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA

Plasmid

#97012

Purpose

Rosa26 targeting vector for intersectional Cre and FLP responsive cassette

Depositor

Russell Ray

Article

Lusk et al BMC Biol. 2022 Jan 28;20(1):28. doi: 10.

Type

Empty backbone

Use

CRISPR and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-K179M

Plasmid

#49188

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Expression

Mutation

K179M

Promoter

T7

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pGIPz-mPD-L1 3SA

Plasmid

#121489

Purpose

Expression of mouse PD-L1 3SA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1 3SA

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

S175A, T179A, S183A

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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ETV3L_pet28a

Plasmid

#131666

Purpose

Bacterial expression of ETS gene ETV3L

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV3L (ETV3L Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBLO ddd64.1

Plasmid

#123121

Purpose

E. coli expression vector for catalytically inactivated deltaproteobacteria CasX

Depositor

Jennifer Doudna, Benjamin Oakes

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

CasX

Use

CRISPR

Tags

Expression

Mutation

Mutated amino acids in catalytic triad to alanine…

Promoter

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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