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Addgene

pCMV5-ratERK2-Q103A
(Plasmid #40816)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40816 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pCMV5
  • Backbone manufacturer
    David Russell, UTSW
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 6200
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MAPK1
  • Alt name
    ERK2
  • Alt name
    p42
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1500
  • Mutation
    Q103A
  • GenBank ID
    NM_053842
  • Entrez Gene
    Mapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
  • Promoter CMV
  • Tag / Fusion Protein
    • His6 (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Melanie Cobb, UTSW

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-ratERK2-Q103A was a gift from Natalie Ahn (Addgene plasmid # 40816 ; http://n2t.net/addgene:40816 ; RRID:Addgene_40816)
  • For your References section:

    The gatekeeper residue controls autoactivation of ERK2 via a pathway of intramolecular connectivity. Emrick MA, Lee T, Starkey PJ, Mumby MC, Resing KA, Ahn NG. Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18101-6. Epub 2006 Nov 17. 10.1073/pnas.0608849103 PubMed 17114285