pCMV5-ratERK2-Q103A
(Plasmid
#40816)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 40816 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCMV5
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Backbone manufacturerDavid Russell, UTSW
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 6200
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMAPK1
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Alt nameERK2
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Alt namep42
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SpeciesR. norvegicus (rat)
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Insert Size (bp)1500
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MutationQ103A
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GenBank IDNM_053842
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Entrez GeneMapk1 (a.k.a. ERK-2, ERT1, Erk2, p42-MAPK)
- Promoter CMV
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Tag
/ Fusion Protein
- His6 (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMelanie Cobb, UTSW
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV5-ratERK2-Q103A was a gift from Natalie Ahn (Addgene plasmid # 40816 ; http://n2t.net/addgene:40816 ; RRID:Addgene_40816) -
For your References section:
The gatekeeper residue controls autoactivation of ERK2 via a pathway of intramolecular connectivity. Emrick MA, Lee T, Starkey PJ, Mumby MC, Resing KA, Ahn NG. Proc Natl Acad Sci U S A. 2006 Nov 28;103(48):18101-6. Epub 2006 Nov 17. 10.1073/pnas.0608849103 PubMed 17114285