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Showing: 10961 - 10980 of 24780 results

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CMV:eGFP-p2A-HA-GPR151

Plasmid

#190133

Purpose

expresses eGFP-p2A-HA-GPR151 (wild-type variant) in mammalian cells

Depositor

Joshua Knowles

Article

Bielczyk-Maczynska et al Nat Commun. 2022 Dec 1;13(1):7408. doi:

Insert

eGFP-p2A-HA-GPR151

Use

Lentiviral

Tags

HA, eGFP, and p2A

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Venus-7aa-ActA-pEGFP-C1

Plasmid

#177405

Purpose

To express Venus fused to mitochondria outer membrane targeting sequence "ActA". Venus faces the cytosol.

Depositor

David Andrews

Article

Osterlund et al Protocol Exchange (2022)

Insert

ActA tail anchor (actA Listeria monocytogenes)

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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ETV2_pet28a

Plasmid

#131665

Purpose

Bacterial expression of ETS gene ETV2

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ETV2 (ETV2 Human)

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Caveolin-3

Plasmid

#68396

Purpose

Fluorescently tagged Caveolin-3 for expression in mammalian cells

Depositor

Rob Parton

Article

Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

Insert

Cav3 (Cav3 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only
pHS0806 His14-bdSUMO-AwaIscB

Plasmid

#176539

Purpose

Bacterial expression of His14-bdSUMO-AwaIscB

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Insert

AwaIscB

Use

CRISPR

Tags

14xHis-bdSUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pRetrosuper Fbw7 shRNA-2

Plasmid

#15661

Depositor

Martin Eilers

Article

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Fbw7 shRNA (FBXW7 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits
pHAGE-UBC-hAAT-IRES-ZsGreen-W

Plasmid

#126702

Purpose

lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

Depositor

Darrell Kotton

Article

Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

Insert

Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UBC

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p37-Strep-HA

Plasmid

#113485

Purpose

Expression of human p37 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

p37 (UBXN2B Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-SomArchon

Plasmid

#126942

Purpose

Expression of SomArchon under CaMKII promoter

Depositor

Edward Boyden

Article

Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

Insert

SomArchon

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-eIF4E K119A

Plasmid

#199440

Purpose

Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

Depositor

Shin-ichi Hoshino

Article

Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

Insert

1977 (EIF4E Human)

Use

Tags

GST-fusion protein

Expression

Bacterial

Mutation

changed lysine 119 to alanine

Promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMOD_B2312

Plasmid

#91070

Purpose

Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers

Use

CRISPR

Tags

Expression

Mutation

Promoter

PvUbi1

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV6-G2-ATP8-V5 (puromycin)

Plasmid

#86852

Purpose

mammalian expression of ATP8 with G2 mitochondrial targeting sequence and V5 tag

Depositor

Matthew O'Connor

Article

Boominathan et al Nucleic Acids Res. 2016 Nov 2;44(19):934

Insert

ATP8 (ATP8 Human)

Use

Tags

ATP5G2 (G2) mitochondrial targeting signal and V5

Expression

Mammalian

Mutation

codon optimized

Promoter

CMV

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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LP455

Plasmid

#90198

Purpose

pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 649-1426

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

NPHP4 (NPHP4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

aa residues 649-1426

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2103b

Plasmid

#91062

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette (doesn't include the promoter) for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette (no promoter) for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Tags

Expression

Mutation

Promoter

CmYLCV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMOD_B2203

Plasmid

#91066

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_C2200), Terminator: none - to be fused to gRNA array in MODULE C

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Tags

Expression

Mutation

Promoter

CmYLCV

Available since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-flag-YTHDF2-C

Plasmid

#52303

Purpose

Flag-tagged YTHDF2 C-terminal domain, from E384 to the end

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Deleted amino acids 1-383

Promoter

T7

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pET 2C-T10-dCasX

Plasmid

#126418

Purpose

E. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification

Depositor

Jennifer Doudna

Article

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Insert

DpbCasX

Use

CRISPR

Tags

His-MBP

Expression

Bacterial

Mutation

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pBabePuro-ApoECDS

Plasmid

#42949

Depositor

Sohail Tavazoie

Article

Pencheva et al Cell. 2012 Nov 21;151(5):1068-82. doi: 1

Insert

Apolipoprotein E (APOE Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 8, 2013

Availability

Academic Institutions and Nonprofits only
LP453

Plasmid

#90184

Purpose

pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 1-839

Depositor

Lotte Pedersen

Article

Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

Insert

NPHP4 (NPHP4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

aa residues 1-839

Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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SYMPK

Plasmid

#156071

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

SYMPK (SYMPK Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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