Showing: 10781 - 10800 of 14149 results
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Plasmid#29371DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationWild type gene including genomic sequence 1432 bp…PromoterAvailabilityAcademic Institutions and Nonprofits only -
pEG593
Plasmid#40119DepositorInsertMex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) (mex-5 Nematode)
UseGateway destination vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationR274E, K318E, Exon 2 recoded to be RNAi-resistantPromoter4.4 kb mex-5 promoter fragmentAvailabilityAcademic Institutions and Nonprofits only -
pEG628
Plasmid#40120DepositorInsertMex-5 (aa1-244) (RNAi resistant) (mex-5 Nematode)
UseGateway destination vectorTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa1-244, Exon 2 recoded to be RNAi-resis…Promoter4.4 kb mex-5 promoter fragmentAvailabilityAcademic Institutions and Nonprofits only -
pEG610
Plasmid#40195DepositorInsertMex-5 (aa1-355) (RNAi resistant) (mex-5 Nematode)
UseTagsDendra2 PAFP (sequence optimized for worm express…ExpressionWormMutationcontains aa 1-355; exon 2 recoded to be RNAi-resi…Promoter4.4 kb mex-5 promoter fragmentAvailabilityAcademic Institutions and Nonprofits only -
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719-2µ-minHIS3
Plasmid#40610DepositorInsertHIS3 (HIS3 Budding Yeast, Synthetic)
UseTagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3AvailabilityAcademic Institutions and Nonprofits only -
pTH734-CEN-minHIS3
Plasmid#38227DepositorInsertHIS3 (HIS3 Budding Yeast, Synthetic)
UseTagsHAExpressionYeastMutationEvery codon of the coding sequence has been repla…PromoterTDH3AvailabilityAcademic Institutions and Nonprofits only -
pRCC-K
Plasmid#81191PurposeExpression of Cas9 and gRNA cassette in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below. and WT - cod…PromoterROX3 and SNP52pAvailabilityAcademic Institutions and Nonprofits only -
GST-CHMP4A (pGEX2T(TEV))
Plasmid#80630PurposeBacterial Expression Vector; TEV cleavable GST-tag Internal ID: WISP06-197DepositorInsertCHMP4A (CHMP4A Human)
UseTagsGSTExpressionBacterialMutationResidues 1-222PromoterAvailabilityAcademic Institutions and Nonprofits only -
pet151D-TOPO ALIX Bro1-V (1-702)
Plasmid#80642PurposeResidues 1-702 of ALIX; Bacterial Expression Vector; TEV cleavable N-term His-tag; Sundquist Lab Internal ID: WISP10-648DepositorInsertALIX Bro1-V (PDCD6IP Human)
UseTagsHis TagExpressionBacterialMutationResidues 1-702PromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA TdL
Plasmid#80938PurposeExpresses Cas9N in mammalian cells; expresses gRNA TdL for Ai9 cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAAV-SMVP-Cas9N-U6-gRNA TdR
Plasmid#80940PurposeExpresses Cas9N in mammalian cells; expresses gRNA TdR for Ai9 cleavage.DepositorInsertCas9N
UseAAV, CRISPR, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pV92.2
Plasmid#78409PurposepKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron constructDepositorInsertsRT-Cas1
Cas2
UseTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRTagsExpressionInsectMutationPromoterpActin (Drosophila)AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_1
Plasmid#78198PurposeExpression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A197C)
UseTagsExpressionBacterialMutationA197C (please see depositors comment below)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
UseTagsExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
UseTagsExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_1
Plasmid#78203PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (G26C)
UseTagsExpressionBacterialMutationG26C (please see depositor comment below)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET22b_EcSSB_2
Plasmid#78204PurposeExpression of single-cysteine variant of single-stranded DNA-binding protein protein, EcSSB, as scaffold for ssDNA biosensorDepositorInsertssb (W88C)
UseTagsExpressionBacterialMutationW88C (please see depositor comment below)PromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pBABE-3xFLAG-OB D166R
Plasmid#79061PurposeRetroviral expression of 3xFLAG tagged, nuclear localized TPP1-OB-RR folding domainDepositorInsertTPP1 (TPP1 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationAspartic acid 166 changed to ArgininePromoterCMVAvailabilityAcademic Institutions and Nonprofits only
Showing: 10781 - 10800 of 14149 results