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Plasmid#29383DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationP174Q mutant gene including genomic sequence 1432…PromoterAvailable sinceMay 23, 2011AvailabilityAcademic Institutions and Nonprofits only -
Sema3e(S)-Fc-His
Plasmid#72146PurposeExpresses the Sema3E protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3e (Sema3e Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NG)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47451PurposeAAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NG_C63)-SID4X
UseAAV; TaleTags2A_phiLOV2.1ExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NI)-NLS-VP64_2A_GFP_WPRE_bGHpA
Plasmid#47448PurposeAAV TALE synthesis backbone with NI half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NI_C63)-VP64
UseAAV; TaleTags2A_GFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NN)-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA
Plasmid#47449PurposeAAV TALE synthesis backbone with NN half repeat; fused to nuclear localization sequence linker and SID4X transcriptional repressor effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NN_C63)-SID4X
UseAAV; TaleTags2A_phiLOV2.1ExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_TALEBB(NG)-NLS-VP64_2A_GFP_WPRE_bGHpA
Plasmid#47447PurposeAAV TALE synthesis backbone with NG half repeat; fused to nuclear localization sequence linker and VP64 transcriptional activator effector domain. Synapsin promoter for neuronal expression.DepositorInsertTALE BB(N136_0.5NG_C63)-VP64
UseAAV; TaleTags2A_GFPExpressionMammalianMutationPromoterhSyn human synapsin (mammalian neuronal expressio…Available sinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmTol-InsUAS-6MTnlstdTomato2A-Dusp6DM-CC
Plasmid#67687PurposeUAS-driven 6xMyc nuclear localized tdTomato-2A-activated Dusp6 in minTol backbone; also with cry:cfp cassetteDepositorInsertdusp6 (dusp6 Zebrafish)
UseTags6xmyc-NLS-tdTomato-2AExpressionMutationS159A, S196APromoterAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
AI-CN590
Plasmid#62596PurposeKCTD13 N-terminus disruption by homolgy-directed repairDepositorInsertCerulean-P2A-PuroR (KCTD13 Human)
UseTALEN; Homology-directed repair donorTagsExpressionMammalianMutationPromoterhEF1alphaAvailable sinceMarch 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
Sema3e(S)-AP-His
Plasmid#72020PurposeExpresses the Sema3E protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3e (Sema3e Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3d(L)-Fc-His
Plasmid#72143PurposeExpresses the Sema3D protein (truncated at cleavage site P3; ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3d (Sema3d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pET30-GBF-E6C
Plasmid#21698DepositorInsertProtein G domain B1 fused C-terminal zinc binding domain of human papillomavirus type 16 (residue 79-142) (E6 Human papillomavirus type 16)
UseTagsProtein G B1 domainExpressionBacterialMutationGB1 (1-56aa) fusion tag with mutations at three p…PromoterAvailable sinceAug. 31, 2009AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.a(S)-Fc-His
Plasmid#72152PurposeExpresses the Sema3G, isoform a protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3g.a (Sema3g Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.z(S)-Fc-His
Plasmid#72153PurposeExpresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3g.z (Sema3g Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3g.z(S)-AP-His
Plasmid#72027PurposeExpresses the Sema3G, isoform z protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3g.z (Sema3g Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3d(S)-Fc-His
Plasmid#72144PurposeExpresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertSema3d (Sema3d Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
Sema3d(S)-AP-His
Plasmid#72018PurposeExpresses the Sema3D protein (truncated at cleavage site P1; ie, short), C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertSema3d (Sema3d Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHL1529
Plasmid#53027PurposePLlacO-1:glgC::gfp, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st2):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsRBS(st2), RBS(st3), and glgC leader contains -61 …ExpressionBacterialMutationPromoterpConNoHind and pLlac0-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTH443-SUP45-G180A
Plasmid#29386DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationG180A mutant gene including genomic sequence 1432…PromoterAvailable sinceJune 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH398-SUP45-G54D
Plasmid#29376DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
UseTagsExpressionYeastMutationG54D mutant gene including genomic sequence 1432 …PromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only