Addgene

154,649 results

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3XFlag-TMCC1

Plasmid

#121045

Purpose

Expresses human TMCC1 in mammalian cells

Depositor

Gia Voeltz

Article

Hoyer et al Cell. 2018 Sep 20;175(1):254-265.e14. do

Insert

Trans membrane and coiled coil domain containing protein 1 (TMCC1 Human)

Use

Tags

3x-Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 WT-pcDNA3.1

Plasmid

#118209

Purpose

Expresses FLAG-OTUB1 WT in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

none

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMOS015: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Plasmid

#163059

Purpose

mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

mito-roGFP2-Orp1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAd5-B1-deltaE1-GFP

Plasmid

#179203

Purpose

Block 1 with deletion in E1 and insertion of GFP expression cassette

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified Block 1 with deletion in E1 and insertion of GFP expression cassette

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pGH335_MS2-AID*Δ-Hygro

Plasmid

#85406

Purpose

lentiviral expression vector for MS2-AID*Δ fusion protein with hygromycin B selectable marker

Depositor

Michael Bassik

Article

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

MS2-AID*Δ and Hygromycin resistance

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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U_(+36GFP)-BirA

Plasmid

#71753

Purpose

U+36GFP-BirA for expression in E.Coli

Depositor

David Liu

Article

Li et al J Am Chem Soc. 2015 Nov 11;137(44):14084

Insert

U_(pos36GFP)_BirA_6XHis_LPETG

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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CDYL

Plasmid

#25509

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

CDYL (CDYL Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pMV762-Peredox-mCherry

Plasmid

#90217

Purpose

Fluorescent reporter for NADH:NAD+ ratios in Mycobacteria.

Depositor

Ashwani Kumar

Article

Bhat et al Front Cell Infect Microbiol. 2016 Nov 8;

Insert

Codon Optimized Peredox-mCherry

Use

Shuttle vector

Tags

Expression

Bacterial

Mutation

Promoter

groEL

Available since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-hMcl-1

Plasmid

#25375

Depositor

Roger Davis

Article

Morel et al Mol Cell Biol. 2009 Jul . 29(14):3845-52

Insert

MCL1 (MCL1 Human)

Use

Tags

V5 and His tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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FRE5

Plasmid

#62377

Purpose

Fluorescent reporter to quantify the ratio of two alternative splicing patterns.

Depositor

Thomas Cooper

Article

Orengo et al Nucleic Acids Res. 2006;34(22):e148. Epu

Insert

dsRed and GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pEF HaloTag-SYB2

Plasmid

#197282

Purpose

Encodes full-length Synaptobrevin 2 (SYB2) with mutations Q76V and F77W to confer resistance to tetanus cleavage.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYB2 and HaloTag (Vamp2 Rat)

Use

Tags

Expression

Mammalian

Mutation

Q76V and F77W

Promoter

EF1alpha

Available since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-eSpCas9(1.1)

Plasmid

#108292

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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mCit-APPAP-gs

Plasmid

#228090

Purpose

Mammalian expression of five coiled coil segments in APPAP arrangment, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, APPAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pAM354 pET28a-GGG-Titin[V15P]-23-K-19-C-15

Plasmid

#226350

Purpose

Expression of proteasome substrate with tail for molecular ruler

Depositor

Andreas Martin

Article

Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

Insert

titin (TTN Human)

Use

Tags

Expression

Bacterial

Mutation

V15P

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Lifeact-EGFP-3xHA (JDW 1322)

Plasmid

#224494

Purpose

Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

Liefact-EGFP-3xHA

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1-Nir3-LNS2

Plasmid

#220087

Purpose

C-terminal PA binding domain of Nir3

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

PITPNM2 (PITPNM2 Human)

Use

Tags

mEGFP-GGGGSHM

Expression

Mammalian

Mutation

amino acids 925-1209

Promoter

CMV

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pIA030

Plasmid

#200424

Purpose

thrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

Depositor

Rut Carballido-López

Article

Altinoglu et al bioRxiv 2023

Insert

LacI

Use

Tags

PAmCherry

Expression

Bacterial

Mutation

Promoter

Ppen

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2164_Nme2-ABE8e-i7

Plasmid

#201517

Purpose

Mammalian expression of domain inlaid Nme2-ABE8e-i7

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2165_Nme2-ABE8e-i8

Plasmid

#201518

Purpose

Mammalian expression of domain inlaid Nme2-ABE8e-i8

Depositor

Erik Sontheimer

Article

Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

Insert

Nme2-ABE8e-i8

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV MP-135 mRFP-3MREs (AAV-alpha)

Plasmid

#206929

Purpose

Alpha cell-specific gene expression from AAV in human pancreatic islets

Depositor

Markus Grompe

Insert

mRFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

MP-135

Available since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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